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应用生态学报 ›› 2016, Vol. 27 ›› Issue (10): 3123-3128.doi: 10.13287/j.1001-9332.201610.027

• 目次 • 上一篇    下一篇

氮素指数施肥对杉木无性系苗生长及养分含量的影响

刘欢1, 王超琦1, 吴家森1,2*, 郭联华2   

  1. 1浙江农林大学浙江省森林生态系统碳循环与固碳减排重点实验室, 浙江临安311300;
    2浙江农林大学亚热带森林培育国家重点实验室培育基地, 浙江临安311300;
  • 收稿日期:2016-05-13 发布日期:2016-10-18
  • 通讯作者: * E-mail: jswu@zafu.edu.cn
  • 作者简介:刘 欢, 女, 1991年生, 硕士研究生. 主要从事农业与资源利用. E-mail: huan04025047@126.com
  • 基金资助:
    本文由国家重点研发计划项目(2016YFD0600304)和浙江省林学一级重中之重学科开放基金项目(KF201317)资助

Effects of exponential N fertilization on the growth and nutrient content in clonal Cunninghamia lanceolata seedlings

LIU Huan1, WANG Chao-qi1, WU Jia-sen1,2*, GUO Lian-hua2   

  1. 1Zhejiang Province Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration, Zhejiang A&F University, Lin’an 311300, China;
    2Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an 311300, China;
  • Received:2016-05-13 Published:2016-10-18
  • Contact: * E-mail: jswu@zafu.edu.cn
  • Supported by:
    This work was supported by the National Major Research Development Plan (2016YFD0600304) and the Zhejiang Provincial Priority First-level Discipline of Forestry Open Funded Project (KF201317).

摘要: 以1年生无性系杉木幼苗为材料,采用温室盆栽方法,设定对照、常规施肥和3个指数施肥共5个处理,施氮总量分别为每株0、0.5、0.5、1.0、2.0 g,施氮20次,间隔为10 d,施氮210 d后,测定杉木的苗高、地径、生物量、叶绿素荧光参数及养分含量.结果表明: 施肥显著促进了杉木无性系的苗高、地径和生物量的增长,以施氮量1.0 g·株-1的指数施肥处理最佳,分别为59.0 cm、6.0 mm、52.99 g·株-1;杉木无性系苗木叶绿素荧光参数特征均表现为指数施肥显著大于常规施肥,其中指数施肥的光化学淬灭系数、表观电子传递速率和PSⅡ的有效量子产量均随施氮总量的增加呈先增加后减小的趋势,在施氮量为1.0 g·株-1时达到最佳,分别为0.89、35.79和0.71.杉木无性系苗木氮含量表现为叶>根>茎.与对照相比,常规施肥根、茎、叶的氮含量分别增加39.6%、16.6%和41.1%,指数施肥根、茎、叶的氮含量分别增加22.6%~81.4%、27.3%~152.6%和73.6%~135.5%.施氮量为1.0和2.0 g·株-1的指数施肥处理杉木幼苗根、茎和叶氮含量均显著大于常规施肥,而不同器官磷含量和钾含量无显著差异.从苗木生长性状、叶绿素荧光参数特征、植株氮磷钾含量等综合考虑,在本试验条件下,施氮量为1.0 g·株-1的指数施肥是杉木无系性苗木温室培育的适宜方法.

Abstract: A pot experiment was conducted in a greenhouse on one-year-old clonal seedlings of Cunninghamia lanceolata. Five treatments were designed including control, conventional fertilization and three exponential fertilization treatments, with N application rates of 0, 0.5, 0.5, 1.0 and 2.0 g·seedling-1, with N applied at 20 times at 10-day interval. The height, ground diameter, biomass, chlorophyll fluorescence and N absorption were measured after 210 days of N application. The results showed that fertilization significantly promoted the seedling height, ground diameter and biomass with the optimal values of 59.0 cm, 6.0 mm, 52.99 g·seedling-1, in exponential fertilization treatment of 1.0 g N·seedling-1. Chlorophyll fluorescence in exponential fertilization treatments was significantly higher than that in conventional fertilization. The photochemical quenching, PSⅡ electron transport rate and photochemical efficiency of PSⅡ in exponential fertilization treatments increased first and then decreased, with the optimal values of 0.89, 35.79 and 0.71 in 1.0 g N·seedling-1 treatment. N contents in different organs of C. lanceolata clonal seedlings were in the order of leaf > root > stem. Compared with the CK, the N contents in root, stem and leaf increased by 39.6%, 16.6% and 41.1% in the conventional fertilization treatment, and by 22.6%-81.4%, 27.3%-152.6% and 73.6%-135.5% in exponential fertilization treatments. N contents in root, stem and leaf in the exponential fertilization treatments of 1.0 and 2.0 g N·seedling-1 were significantly higher than those in the conventional fertilization treatment. P and K contents were not significantly different among the different organs. Considering the characteristics of seedling growth, chlorophyll fluorescence, the contents of N, P and K, the exponential fertilization treatment of 1.0g N· seedling-1 was the best in cultivating C. lanceolata clonal seedlings in this experiment.