人参连作根际土壤中酚酸物质对人参锈腐病菌的化感效应

doi:10.13287/j.1001-9332.201611.002

应用生态学报 ›› 2016, Vol. 27 ›› Issue (11): 3616-3622.doi: 10.13287/j.1001-9332.201611.002

人参连作根际土壤中酚酸物质对人参锈腐病菌的化感效应

李自博, 周如军, 解宇娇, 傅俊范^*

沈阳农业大学植物保护学院, 沈阳 110866

收稿日期:2016-03-04 出版日期:2016-11-18 发布日期:2016-11-18
通讯作者: E-mail: fujunfan@163.com
作者简介:李自博,男,1989年生,博士研究生.主要从事药用植物病理学研究. E-mail: dabodabo@126.com
基金资助:
本文由农业部行业科技专项(201303111))资助

Allelopathic effects of phenolic compounds of ginseng root rhizosphere on Cylindrocarpon destructans.

LI Zi-bo, ZHOU Ru-jun, XIE Yu-jiao, FU Jun-fan

College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China

Received:2016-03-04 Online:2016-11-18 Published:2016-11-18
Contact: E-mail: fujunfan@163.com
Supported by:
This study was supported by the Scientific Special Project of Department of Agriculture of China (201303111).

摘要/Abstract

摘要： 采用高效液相色谱法对人参连作根际土壤中的酚酸物质进行了分离鉴定,检测发现人参根际土壤中含有没食子酸、水杨酸、3-苯基丙酸、苯甲酸和肉桂酸5种酚酸物质.采用外源添加法研究该5种酚酸物质对人参锈腐病菌的化感效应.结果表明: 5种酚酸对人参锈腐病菌的菌丝生长和孢子萌发都表现出高浓度抑制、低浓度促进的作用.没食子酸、水杨酸和苯甲酸在0.5 mmol·L^-1处理浓度下,3-苯基丙酸和肉桂酸在0.05 mmol·L^-1处理浓度下,均能够显著促进人参锈腐病菌菌丝生长和孢子萌发,并显著加重人参锈腐病病害严重度.

关键词: 人参根际土壤, 酚酸物质, 人参锈腐病, 化感效应, 连作障碍

Abstract: In this study, five phenolic compounds of ginseng rhizosphere soil were identified by HPLC, including gallic acid, salicylic acid, 3-phenylpropionic acid, benzoic acid and cinnamic acid. The results show that five phenolic compounds inhibited mycelium growth and spore germination at high concentration, but promoted mycelium growth and spore germination at low concentration. Gallic acid, salicylic acid, benzoic acid of 0.5 mmol·L^-1 and 3-phenylpropionic acid, cinnamic acid of 0.05 mmol·L^-1 could significantly promote the spore germination, mycelium growth and disease severity of Cylindrocarpon destructans.

Key words: root rot of ginseng, allelopathic effect, phenolic compounds, ginseng rhizosphere, continuous cropping obstacle

李自博, 周如军, 解宇娇, 傅俊范. 人参连作根际土壤中酚酸物质对人参锈腐病菌的化感效应[J]. 应用生态学报, 2016, 27(11): 3616-3622.

LI Zi-bo, ZHOU Ru-jun, XIE Yu-jiao, FU Jun-fan. Allelopathic effects of phenolic compounds of ginseng root rhizosphere on Cylindrocarpon destructans.[J]. Chinese Journal of Applied Ecology, 2016, 27(11): 3616-3622.

参考文献

[1] Cao Z-Q (曹志强), Jin H (金慧), Xu Y-H (许永华), et al. Research of successive transplants on conti-nuous cropping and farmland of ginseng. Ginseng Research (人参研究), 2005, 17(1): 1-9 (in Chinese)
[2] Wu L-J (吴连举), Zhao Y-H (赵亚会), Guan Y-M (关一鸣), et al. A review on studies of the reason and control methods of succession cropping obstacle of Panax ginseng C.A.Mey. Special Wild Economic Animal and Plant Research (特产研究), 2008, 30(2): 68-72 (in Chinese)
[3] Jian Z-Y (简在友), Wang W-Q (王文全), Meng L (孟丽), et al. Research of successive cropping obstacle of Panax medicinal plant. Modern Chinese Medicine (中国现代中药), 2008, 10(6): 3-5 (in Chinese)
[4] Chen C-B (陈长宝), Wang Y-Y (王艳艳), Liu J-Y (刘继永), et al. Identification of allelochemical from the rhizosphere soil of ginseng. Special Wild Economic Animal and Plant Research (特产研究), 2006, 28(2): 12-14 (in Chinese)
[5] Li X (李夏), Zhou B-L (周宝利), Chen S-L (陈绍莉), et al. Effects of the autotoxic substance in eggplants on pepper seed germination and Fusarium oxysporum f. sp. Acta Ecologica Sinica (生态学报), 2009, 29(2): 960-965 (in Chinese)
[6] Liu P (刘苹), Zhao H-J (赵海军), Tang C-H (唐朝辉), et al. Effects of continuous cropping on root exudates of different resistance peanut (Arachis hypogaea L.) varieties and allelochemicals content in soil. Chinese Journal of Oil Crop Sciences (中国油料作物学报), 2015, 37(4):467-474 (in Chinese)
[7] Wu F-Z (吴凤芝), Zhao F-Y (赵凤艳), Ma F-M (马凤鸣). Phenolic compound and its allelopathy. Journal of Northeast Agricultural University (东北农业大学学报), 2001, 32(4): 313-319 (in Chinese)
[8] Jasicka-Misiak Z, Wieczorek PP, Kafarski P, et al. Crotonic acid as a bioactive factor in carrotseeds. Phytochemistry, 2005, 66: 1485-1491
[9] Yang R-X (杨瑞秀), Gao Z-G (高增贵), Yao Y (姚远), et al. Allelopathic effects of phenolic compounds of melon root exudates on Fusarium oxysporum f. sp. me-lonis. Chinese Journal of Applied Ecology (应用生态学报), 2014, 25(8): 1-6 (in Chinese)
[10] Jiao X-L (焦晓林), Yang J-X (杨家学), Gao W-W (高微微), et al. Allelopathic effects of phenolic compounds from American ginseng cultivated soil on four crops. Modern Chinese Medicine (中国现代中药), 2015, 17(5): 479-484 (in Chinese)
[11] Lara-Nunez A, Romero-Romero T, Ventura JL, et al. Allelochemical stress causes inhibition of growth and oxidative damage in Lycopersicon esculentum Mill. Plant, Cell and Environment, 2009, 29: 2009-2016
[12] Xue C-Y (薛成玉), Wu F-Z (吴凤芝), Wang H-C (王洪成), et al. The summary of reciprocity between phenolic acids and soil microorganism. Heilongjiang Agricultural Science (黑龙江农业科学), 2005(3): 45-47 (in Chinese)
[13] Yan X-R (严雪瑞), Fu J-F (傅俊范). A summary of researches on cylindrocarpon and panax root rust-rot. Journal of Shenyang Agricultural University (沈阳农业大学学报), 2002, 33(1): 71-75 (in Chinese)
[14] Rahman M, Punja ZK. Factors influencing development of root rot on ginseng caused by Cylindrocarpon destructans. Phytopathology, 2005, 95: 1381-1390
[15] Fu J-F (傅俊范). Medicinal Plant Pathology. Beijing: China Agriculture Press, 2007 (in Chinese)
[16] Martens D. Identification of phenolic acid composition of alkali-extracted plants and soils. Soil Science Society of America Journal, 2002, 66: 1240-1248
[17] Hu Y-S (胡元森), Li C-X (李翠香), Du G-Y ( 杜国营), et al. Allelochemical identification and effects of cucumber root exudates. Ecology and Environment (生态环境), 2007, 16(3): 954-957 (in Chinese)
[18] Zhuang J-H (庄敬华), Yang C-C (杨长成), Gao Z-G (高增贵), et al. Simulation of allelopathy of melon under continuous cropping. Plant Protection (植物保护), 2008, 34(5): 137-139 (in Chinese)
[19] Blum U, Shafer SS. Microbial populations and phenolic acid in soil. Soil Biology and Biochemistry, 1988, 20: 793-800
[20] Rahman M, Punja ZK. Influence of iron on cylindrocarpon root rot development on ginseng. Ecology and Epidemiology, 2006, 96: 1179-1187
[21] Yang J-X (杨家学), Gao W-W (高微微). Effects of phenolic allelochemicals on the pathogen of Panax qui-quefolium L. Chinese Agricultural Science Bulletin (中国农学通报), 2009, 25(9): 207-211 (in Chinese)
[22] Blum U, Thomas M. Relationships between phenolic acid concentrations, transpiration, water utilization, leaf area expansion, and uptake of phenolic acid: Nutrient culture studies. Journal of Chemical Ecology, 2005, 31: 1907-1932
[23] Li P-D (李培栋), Wang X-X (王兴祥), Li Y-L (李奕林), et al. The contents of phenolic acids in continuous cropping peanut and their allelopathy. Acta Ecologica Sinica (生态学报), 2010, 30(8): 2128-2134 (in Chinese)
[24] Liang C-Q (梁春启), Zhen W-C (甄文超), Zhang C-Y (张承胤), et al. Determination of phenolic acids in decomposing products of maize straw and their allelopathy on pathogens of wheat soil-borne disease. Chinese Agricultural Science Bulletin (中国农学通报), 2009, 25(2): 210-213 (in Chinese)
[25] Yu JQ, Ye SF, Zhang MF, et al. Effects of root exudates and aqueous root extracts of cucumber (Cucumis sativus) and allelochemicals on photosynthesis and antioxidant enzymes in cucumber. Biochemical Systematics and Ecology, 2003, 31: 129-139
[26] Zhen W-C (甄文超), Wang X-Y (王晓燕), Kong J-Y (孔俊英), et al. Determination of phenolic acids in root exudates and decomposing products of strawberry and their allelopathy. Journal of Agricultural University of Hebei (河北农业大学学报), 2004, 27(4): 74-78 (in Chinese)

人参连作根际土壤中酚酸物质对人参锈腐病菌的化感效应

Allelopathic effects of phenolic compounds of ginseng root rhizosphere on Cylindrocarpon destructans.

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