欢迎访问《应用生态学报》官方网站,今天是 分享到:

应用生态学报 ›› 2016, Vol. 27 ›› Issue (11): 3427-3434.doi: 10.13287/j.1001-9332.201611.016

• • 上一篇    下一篇

氮添加对湿地松林土壤水解酶和氧化酶活性的影响

张闯1,2, 邹洪涛1, 张心昱2*, 寇亮2, 杨洋2,3, 孙晓敏2, 李胜功2, 王辉民2   

  1. 1沈阳农业大学土地与环境学院, 沈阳 110866;
    2中国科学院地理科学与资源研究所生态系统网络观测与模拟重点实验室, 北京 100101;
    3哈尔滨师范大学地理科学学院, 哈尔滨 150025
  • 收稿日期:2016-05-09 出版日期:2016-11-18 发布日期:2016-11-18
  • 通讯作者: E-mail: zhangxy@igsnrr.ac.cn
  • 作者简介:张 闯,男,1992年生,硕士研究生. 主要从事土壤碳、氮循环及微生物机制研究. E-mail: zhcnd10@126.com
  • 基金资助:
    本文由国家自然科学基金项目(31130009, 31290222, 41571251)资助

Effects of nitrogen additions on soil hydrolase and oxidase activities in Pinus elliottii plantations.

ZHANG Chuang1,2, ZOU Hong-tao1, ZHANG Xin-yu2*, KOU Liang2, YANG Yang2,3, SUN Xiao-min2, LI Sheng-gong2, WANG Hui-min2   

  1. 1College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China;
    2Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;
    3College of Geographic Science, Harbin Normal University, Harbin 150025, China
  • Received:2016-05-09 Online:2016-11-18 Published:2016-11-18
  • Contact: E-mail: zhangxy@igsnrr.ac.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (31130009, 31290222, 41571251).

摘要: 通过3个水平野外氮添加控制试验(0、40、120 kg N·hm-2·a-1),研究氮添加对亚热带湿地松林土壤水解酶和氧化酶活性的影响.结果表明: 氮添加显著抑制了土壤有机质中碳、氮、磷水解酶和氧化酶的活性,导致β-1,4-葡糖苷酶(BG)、纤维素二糖水解酶(CBH)、β-1,4-乙酰基-葡糖胺糖苷酶(NAG)、过氧化物酶(PER)活性下降16.5%~51.1%,并且高水平氮添加对酶活性抑制效果更明显;氮添加导致α-1,4-葡糖苷酶(aG)、β-1,4-木糖苷酶(BX)、酸性磷酸酶(AP)、多酚氧化酶(PPO)活性降低14.5%~38.6%,不同水平氮添加处理间差异不显著.土壤酶活性存在明显的季节性差异,BG、NAG、BX、CBH、AP、PPO活性表现为3月>6月>10月,aG、PER活性表现为10月>3月>6月.多数土壤水解酶和氧化酶与pH呈显著正相关,与NO3--N含量呈显著负相关,表明氮添加导致pH降低和土壤中硝化作用增强,抑制了土壤水解酶和氧化酶活性.氮添加不利于亚热带土壤有机质的矿化和周转,并且随着氮添加量的增加,效果更明显.

关键词: 水解酶活性, 红壤, 氧化酶活性, 氮添加, 湿地松

Abstract: We evaluated responses of hydrolase and oxidase activities in a subtropical Pinus elliottii plantation through a nitrogen (N) addition field experiment (dosage level: 0, 40, 120 kg N·hm-2·a-1). The results showed that N additions significantly decreased the carbon, nitrogen and phosphorus related hydrolase and oxidase activities. The activities of β-1,4-glucosidase (BG), cellobiohydrolase (CBH), β-1,4-N-acetylglucosaminidase (NAG) and peroxidase (PER) activities were decreased by 16.5%-51.1% due to N additions, and the decrease was more remarkable in the higher N addition treatment. The activities of α-1,4-glucosidase (aG), β-1,4-xylosidase (BX), acid phosphatase (AP) and phenol oxidase (PPO) were decreased by 14.5%-38.6% by N additions, however, there was no significant difference among the different N addition treatments. Soil enzyme activities varied obviously in different seasons. The activities of BG, NAG, BX, CBH, AP and PPO were in the order of March > June > October, and aG and PER activities were in the order of October > March > June. Most of the soil hydrolase and oxidase activities were positively correlated with soil pH, but negatively with NO3--N content. It indicated that N additions inhibited soil hydrolase and oxidase activities by reducing soil pH and increasing soil nitrification. N additions inhibited the soil organic matter mineralization and turnover in the subtropical area, and the effects were obvious with the increasing dosage of N additions.

Key words: Pinus elliottii, red soil, nitrogen addition, hydrolase activity, oxidase activity