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应用生态学报 ›› 2017, Vol. 28 ›› Issue (3): 957-965.doi: 10.13287/j.1001-9332.201703.007

• 目次 • 上一篇    下一篇

豆科作物间作燕麦对土壤固氮微生物丰度和群落结构的影响

杨亚东1, 冯晓敏1,2, 胡跃高1, 任长忠3, 曾昭海1*   

  1. 1中国农业大学农学院, 北京 100193
    2中国农业科学院作物科学研究所/农业部作物生理生态重点实验室, 北京 100081
    3吉林省白城市农业科学院, 吉林白城 137000
  • 收稿日期:2016-06-15 发布日期:2017-03-18
  • 通讯作者: *E-mail: zengzhaohai@cau.edu.cn
  • 作者简介:杨亚东,男,1987年生,博士研究生.主要从事土壤微生物生态学研究.E-mail:yadong_tracy@sina.com
  • 基金资助:
    本研究由国家现代农业产业技术体系建设专项(CARS-08-B-1)和公益性行业(农业)科研专项(201503121-11,201503120)资助

Effects of legume-oat intercropping on abundance and community structure of soil N2-fixing bacteria

YANG Ya-dong1, FENG Xiao-min1,2, HU Yue-gao1, REN Chang-zhong3, ZENG Zhao-hai1*   

  1. 1College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
    2Institute of Crop Science, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, Beijing 100081, China
    3Baicheng Academy of Agricultural Sciences, Baicheng 137000, China
  • Received:2016-06-15 Published:2017-03-18
  • Contact: *E-mail: zengzhaohai@cau.edu.cn
  • Supported by:
    This study was supported by the China Agriculture Research System Project (CARS-08-B-1) and the Special Fund for Agro-scientific Research in the Public Interest (201503121-11, 201503120)

摘要: 利用荧光定量PCR和高通量测序的研究方法,比较了3种种植模式(燕麦单作,O;大豆/燕麦,OSO;绿豆/燕麦,OMO)对燕麦土壤固氮微生物数量和群落组成的影响.结果表明: 与大豆和绿豆间作显著改变了燕麦土壤的理化性质.燕麦土壤固氮微生物nifH基因拷贝数为每克干土1.75×1010~7.37×1010,拔节期和成熟期OSO和OMO中nifH基因拷贝数分别是O中的2.18、2.64和1.92、2.57倍,且各处理成熟期nifH基因拷贝数显著低于拔节期.样品稀释性曲线和覆盖度结果表明,各样品nifH基因测序结果可靠.与绿豆间作显著提高了燕麦土壤nifH基因的多样性.各样品固氮微生物属水平上优势类群均为Azohydromonas、固氮菌属、慢生根瘤菌属、Skermanella和在属水平上无法归类的固氮微生物,但各优势类群相对丰度存在差异.样品OTU的venn分布和主成分分析显示,拔节期和成熟期nifH基因群落结构存在差异,两个生育时期OSO和OMO具有更相似的nifH基因群落结构.表明与大豆和绿豆间作可显著提高燕麦土壤固氮微生物的数量,并影响固氮微生物的群落组成.

Abstract: In this study, real-time PCR and high-throughput sequencing approaches were employed to investigate the abundance and community structure of N2-fixing bacteria in a field experiment with three planting patterns (Oat monoculture, O; Soybean-oat intercropping, OSO; Mung bean-oat intercropping, OMO). The results showed that soil chemical properties varied significantly in different soil samples (P<0.05). The abundance of nifH gene varied from 1.75×1010 to 7.37×1010 copies·g-1 dry soil in all soil samples. The copy numbers of nifH gene in OSO and OMO were 2.18, 2.64, and 1.92, 2.57 times as much as that in O at jointing and mature stages, with a significant decline from jointing to mature stage for all treatments (P<0.05). Rarefaction curve and cove-rage results proved the nifH gene sequencing results were reliable, and the diversity index showed that the N2-fixing bacteria diversity of OSO was much higher than that of O. Azohydromonas, Azotobacter, Bradyrhizobium, Skermanella and other groups that could not be classified are the dominant genera, with significant differences in proportion of these dominant groups observed among all soil samples (P<0.05). Venn and PCA analysis indicated that there were greater differences of nifH gene communities between jointing and mature stages; however, the OSO and OMO had similar communities in both stages. All these results confirmed that legume-oat intercropping significantly increased the abundance and changed the community composition of N2-fixing bacteria in oat soils.