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应用生态学报 ›› 2017, Vol. 28 ›› Issue (6): 2033-2039.doi: 10.13287/j.1001-9332.201706.014

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溴氰菊酯对真鲷肝胰脏组织及细胞DNA的损伤

郑惠东*   

  1. 福建省水产研究所/福建省海洋生物增养殖与高值化利用重点实验室, 福建厦门 361013
  • 收稿日期:2016-11-03 发布日期:2017-06-18
  • 通讯作者: *E-mail:364145414@qq.com
  • 作者简介:郑惠东,男,1976年生,高级工程师.主要从事海水养殖与渔业生态环境研究.E-mail:364145414@qq.com
  • 基金资助:
    本文由国家海洋局海洋公益性行业科研专项(201305043-4, 201505034-4)

Damage of deltamethrin on tissue and cell DNA in hepatopancreas of Pagrosomus major

ZHENG Hui-dong   

  1. Fisheries Research Institute of Fujian/Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, Fujian, China
  • Received:2016-11-03 Published:2017-06-18
  • Contact: *E-mail:364145414@qq.com
  • Supported by:
    This work was supported by the Marine Scientific Research Project of the State Oceanic Administration (201305043-4,201505034-4)

摘要: 为研究溴氰菊酯亚急性染毒对真鲷肝胰脏的毒性作用,将真鲷分为5组进行不同剂量半静置染毒.染毒25 d后取真鲷肝胰脏进行组织切片显微观察,并采用彗星试验技术对其肝胰脏细胞进行DNA损伤分析.结果表明: 在0.025、0.125、0.250和0.375 μg·L-1暴露浓度下,真鲷肝胰脏组织出现了不同程度的淤血、细胞核浓缩、细胞坏死等病理性损伤,且染毒浓度越高组织细胞损伤越显著.与空白对照组相比,各染毒浓度组肝胰脏细胞DNA均有不同程度损伤,彗星拖尾率、彗尾DNA相对含量、Olive距等指标均与对照有显著性差异.一元回归分析表明,染毒浓度与拖尾率、彗星尾长等参数呈极显著正相关; 染毒浓度与各指标均呈线性关系,回归方程拟合度(R)极高,范围为0.909~0.996.表明溴氰菊酯对真鲷肝胰脏组织和细胞DNA均可产生不同程度损伤,且损伤程度与染毒浓度之间具有高度线性相关关系.

Abstract: In order to study the toxic effect of deltamethrin on the hepatopancreas of Pagrosomus major with sub-acute exposure, Pagrosomus major were divided into 5 groups with different doses of semi-static exposure. The damage of the hepatopancreas tissue of Pagrosomus major was analyzed by microscopy observation and the DNA damage of the cells was analyzed by comet assay technique after 25 days exposure. The results showed that different degrees of congestion, nuclear enrichment and cell necrosis appeared in hepatopancreas of 0.025, 0.125, 0.250, 0.375 μg·L-1 exposure concentrations. The higher the exposure concentration, the variations of tissue and cell were more significant. DNA damages in the hepatopancreas cells of all exposed group were observed. The co-met rate, percentage of tail DNA and the olive moment were significantly different from the control group (P<0.05). Linear regression analysis showed that the exposure concentration was significantly positively correlated with the tailing rate and the tail length (P<0.01). The indexes were significantly related to the concentration of deltamethrin exposure with the coefficient of determination between 0.909-0.996. It was suggested that deltamethrin could cause various degree of damage to the tissue and cellular DNAs in the hepatopancreas of Pagrosomus major in a linear responsive manner.