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应用生态学报 ›› 2017, Vol. 28 ›› Issue (6): 1779-1785.doi: 10.13287/j.1001-9332.201706.031

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萘对川西亚高山森林土壤呼吸、养分和酶活性的影响

杨帆1,2, 杨万勤1,2, 吴福忠1,2, 王惠1,2, 蓝丽英1,2, 刘育伟1,2, 郭彩虹1,2, 谭波1,2,3*   

  1. 1四川农业大学生态林业研究所/长江上游林业生态工程四川省重点实验室/高山森林生态系统定位研究站/水土保持与荒漠化防治省级重点实验室, 成都 611130
    2长江上游生态安全协同创新中心, 成都 611130
    3绵阳师范学院, 生态安全与保护四川省重点实验室, 四川绵阳 621000
  • 收稿日期:2016-11-09 发布日期:2017-06-18
  • 通讯作者: *E-mail:bobotan1984@163.com
  • 作者简介:杨帆,男,1992年生,硕士研究生.主要从事森林生态和土壤生态研究.E-mail:yangfansicau@163.com
  • 基金资助:
    本文由国家自然科学基金项目(31500509,31570445,31500358)、高等学校博士学科点专项科研基金项目(20135103110002)、四川省教育厅青年项目(14ZB0001)、生态安全与保护四川省重点实验室开放基金项目(ESP201309, ESP1404)和四川农业大学科研兴趣培养项目(20150214)资助

Effects of naphthalene on soil respiration, nutrients and enzyme activities in the subalpine forest of western Sichuan, China

YANG Fan1,2, YANG Wan-qin1,2, WU Fu-zhong1,2, WANG Hui1,2, LAN Li-ying1,2, LIU Yu-wei1,2, GUO Cai-hong1,2, TAN Bo1,2,3*   

  1. 1Institute of Ecology &Forestry, Sichuan Agricultural University/Sichuan Province Key Laboratory of Forestry Ecological Engineering in Upper Reaches of Yangtze River/Alpine Forest Ecosystem Research Station/Sichuan Pro-vince Key Laboratory of Soil and Water Conservation and Desertification Control, Chengdu 611130, China
    2Collaborative Innovation Center of Ecological Security in the Upper Reaches of Yang-tze River, Chengdu 611130, China
    3Sichuan Province Key Laboratory of Ecological Security and Protection, Mianyang 621000, Sichuan, China
  • Received:2016-11-09 Published:2017-06-18
  • Contact: *E-mail:bobotan1984@163.com
  • Supported by:
    This word was supported by the National Natural Science Foundation of China (31500509, 31570445, 31500358), the Specialized Research Fund for the Doctoral Program of Universities (20135103110002), the Youth Project of Sichuan Provincial Department of Education (14ZB0001), the Open Foundation Project of Key Laboratory of Ecological Security and Protection in Sichuan Province (ESP201309, ESP1404), and the Project of Research Interest Training in Sichuan Agricultural University (20150214)

摘要: 萘作为土壤动物化学抑制剂已在土壤动物生态功能的研究中广泛使用,但其非目标效应使其应用仍存在很大的不确定性.为了了解在亚高山森林土壤应用萘抑制土壤动物群落是否存在非目标效应,以青藏高原东缘的川西亚高山森林土壤为研究对象,采用微缩试验研究了萘对土壤呼吸速率、养分含量和酶活性的短期影响.结果表明: 萘处理显著抑制了培养0~10 d的土壤呼吸速率,随后(24~52 d)表现出明显的促进作用.萘处理显著影响了土壤铵态氮和硝态氮含量的动态变化,萘处理铵态氮和硝态氮含量分别以培养的3和17 d最高,对照则以培养的45 d和结束时的52 d最高.萘处理土壤可溶性碳含量在培养3 d急剧增加后迅速降低,对照则略有升高后降低,而萘处理和对照的可溶性氮含量均表现为先升高后降低.萘处理和对照的土壤酶活性均具相似的动态规律,两者的脲酶、硝酸还原酶和亚硝酸还原酶活性分别在培养45、38和10 d至最高.萘处理和采样时间的交互作用显著影响了土壤呼吸速率,以及土壤铵态氮、硝态氮和可溶性氮含量,但对可溶性碳含量、蔗糖酶、硝酸还原酶和亚硝酸还原酶活性的影响不显著.萘作为驱虫剂的非目标效应可能在短期内对川西亚高山森林土壤的氮循环过程产生强烈的影响.

Abstract: As a biocide to reduce soil and litter faunal populations in field experiments, naphthalene has been widely used in the study of ecological functions of soil fauna, but the non-target effects of naphthalene bring about enormous uncertainty to its application. In order to understand whether there were non-target effects of naphthalene in subalpine forest soil, soil in the subalpine forests of west Qinghai-Tibet Plateau was taken as study object. The short-term responses of soil respiration rate, nutrient content and enzyme activity to naphthalene were studied in microcosms. The results showed that soil respiration rate was significantly suppressed by application of naphthalene within 0-10 days, and then showed a significant promotion effect. Naphthalene significantly affected the dynamics of soil NH4+-N and NO3--N contents. With application of naphthalene, the highest contents of NH4+-N and NO3--N occurred at the 3rd and 7th day, respectively. But they were observed at the 45th and 52nd day with no-naphthalene, respectively. Moreover, soil dissolved carbon content in the naphthalene microcosms showed a sharp increase and then decrease dynamic at the 3rd day, while small change was detected in the no-naphthalene microcosms. Dissolved nitrogen content in both the naphthalene and no-naphthalene microcosms showed an increase at first and then decreased subsequently during the study period. Similar dynamics were found for the soil enzyme activities in both the naphthalene and no-naphthalene microcosms. The highest activities of urease, nitrate reductase and nitrite reductase in both the naphthalene and no-naphthalene microcosms were at the 45th, 38th and 10th day, respectively. In addition, the interaction of naphthalene treatment and sampling time had significant effects on soil respiration rate, the contents of NH4+-N, NO3--N and dissolved nitrogen, but had no significant effects on soil dissolved carbon content, and the activities of invertase, nitrate reductase and nitrite reductase. In a short time, the non-target effect of naphthalene as a biocide to reduce soil fauna abundance might have an important influence on the soil nitrogen cycling in subalpine forest of western Sichuan.