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应用生态学报 ›› 2017, Vol. 28 ›› Issue (7): 2353-2360.doi: 10.13287/j.1001-9332.201707.036

• 综合评述 • 上一篇    下一篇

铵盐和硝酸盐稳定同位素丰度测定方法及其应用案例

刘冬伟1, 图影1,2, 方运霆1,3*   

  1. 1中国科学院沈阳应用生态研究所森林生态与管理重点实验室, 沈阳 110016
    2中国科学院大学, 北京 100049
    3中国科学院清原森林生态系统观测研究站, 沈阳 110016
  • 收稿日期:2017-06-19 修回日期:2017-06-23 发布日期:2017-07-18
  • 通讯作者: *mail:fangyt@iae.ac.cn
  • 作者简介:刘冬伟,女,1987年生,博士,助理研究员.主要从事氮素生物地球化学循环研究.E-mail:dwliu@iae.ac.cn
  • 基金资助:
    本文由国家重点研发计划项目(2016YFA0600802)、中国科学院战略性先导科技专项(B类;XDB15020200)、国家自然科学基金项目(31422009,31600358)和中国科学院前沿科学重点研究项目(QYZDB-SSW-DQC002)资助

Isotope analysis of ammonium and nitrate: A review on measured methods and their application

LIU Dong-wei1, TU Ying1,2, FANG Yun-ting1,3*   

  1. 1Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
    2University of Chinese Academy of Sciences, Beijing 100049, China
    3Qingyuan Forest CERN, Chinese Academy of Sciences, Shenyang 110016, China
  • Received:2017-06-19 Revised:2017-06-23 Published:2017-07-18
  • Contact: *mail:fangyt@iae.ac.cn
  • Supported by:
    The work was supported by the National Key Research and Development Program of China (2016YFA0600802), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB15020200), the National Natural Science Foundation of China (31422009, 31600358), and Key Research Program of Frontier Sciences, Chinese Academy of Sciences (QYZDB-SSW-DQC002).

摘要: 综述了过去几十年来铵盐和硝酸盐稳定同位素丰度测定方法的历史发展变化,分析了各种方法的优缺点,并对新方法作了介绍和推荐.目前铵盐稳定同位素丰度的最新测定方法为次溴酸盐氧化结合羟胺还原法,硝酸盐氮氧同位素丰度主流的测定方法为反硝化细菌法和镉粉叠氮酸还原化学法.这些方法的主要共同特点是以N2O为分析物,分析精度高,对样品的含氮量需求小,一般只需要10~60 nmol N,适用于低浓度样品.新方法的建立对于国内外开展氮素循环研究将起到极大的推动作用.

Abstract: In the past several decades, a variety of methods have been developed for measuring the isotopic composition of ammonium (δ15N) and nitrate (δ15N and δ18O). This review summarized the advantages and disadvantages of these methods. Nowadays, the most popular method for measu-ring δ15N of ammonium is the combined hypobromite (BrO-) and hydroxylamine (NH2OH) me-thod, while for δ15N and δ18O of nitrate is the denitrifier method and the sodium azide (NaN3) me-thod. These methods convert NH4+ or NO3- into nitrous oxide (N2O) and measure its isotopic compositions, with higher analytical precision because of the lower background concentration of atmospheric N2O. Accordingly, these methods are suitable for the samples with lower N concentration, and normally require 10-60 nmol N. The development of new methods for measuring N isotopic composition has greatly stimulated the studies in nitrogen cycling worldwide.