欢迎访问《应用生态学报》官方网站,今天是 分享到:

应用生态学报 ›› 2017, Vol. 28 ›› Issue (9): 3003-3010.doi: 10.13287/j.1001-9332.201709.033

• 目次 • 上一篇    下一篇

纳米Fe3O4对生菜生长及土壤细菌群落结构的影响

徐江兵1,2*, 王艳玲1, 罗小三1, 冯有智2   

  1. 1南京信息工程大学应用气象学院生态气象环境中心, 南京 210044
    2中国科学院南京土壤研究所, 土壤与农业可持续发展重点实验室, 南京 210008
  • 收稿日期:2017-02-06 出版日期:2017-09-18 发布日期:2017-09-18
  • 通讯作者: * E-mail: jbxu@nuist.edu.cn
  • 作者简介:徐江兵,男,1981年生,博士,讲师.主要从事微生物生态及废弃物资源化研究. E-mail: jbxu@nuist.edu.cn
  • 基金资助:

    本文由江苏省自然科学基金项目(BK20140991)、国家自然科学基金项目(41501264,41571286)、土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所)(Y412201441)和南京信息工程大学人才启动基金项目资助

Influence of Fe3O4 nanoparticles on lettuce (Lactuca sativa L.)growth and soil bacterial community structure.

XU Jiang-bing1,2, WANG Yan-ling1, LUO Xiao-san1, FENG You-zhi2   

  1. 1International Center for Ecology, Meteorology and Environment, School of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China;
    2State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
  • Received:2017-02-06 Online:2017-09-18 Published:2017-09-18
  • Contact: * E-mail: jbxu@nuist.edu.cn
  • Supported by:

    This work is supported by the Natural Science Foundation of Jiangsu Province (BK20140991), the National Natural Science Foundation of China (41501264, 41571286), the Research Fund of State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science (Y412201441) and the Startup Foundation for Advanced Talents of Nanjing University of Information Science

摘要: 纳米Fe3O4的广泛应用增加了其暴露到农田环境的可能性,因此亟待研究纳米Fe3O4对农业生态环境的影响.本研究采用盆栽试验方式,研究不同浓度纳米Fe3O4颗粒(1、10、100 mg·kg-1)对生菜生长及土壤细菌群落的影响,并与相应浓度的普通Fe3O4处理进行对比.通过测定植物光合速率常数、植株Fe含量来评价植物生长;采用高通量测序技术研究土壤细菌群落结构及组成.结果表明: 不同浓度纳米Fe3O4的影响不同.低浓度纳米Fe3O4能提高植物生物量,增强植物叶片光合速率,增加土壤中黄单胞菌目的相对丰度,降低蓝细菌、鞘脂杆菌纲的相对丰度,但对群落多样性指数影响不显著.高浓度纳米Fe3O4抑制作物生长,提高植株中Fe积累及土壤电导率,降低细菌群落系统发育多样性,降低黄单胞菌目、鞘脂杆菌纲相对丰度,增加蓝细菌相对丰度.此外,一些土壤功能微生物对纳米Fe3O4及普通Fe3O4处理的响应也存在差异,说明不同粒径及浓度的Fe3O4均会对土壤微生物群落产生影响,并可能影响地上部分植物性状.因此,在评估纳米颗粒的生物学效应时需较多关注土壤微生物.

Abstract: With extensive application of Fe3O4 nanoparticles in a variety of fields, the possibility of their exposure to agricultural environment is growing. This raises the concerns on their effects on environment. In this investigation, we carried out a pot experiment to investigate the impacts of diffe-rent concentrations of Fe3O4 nanoparticles (1, 10, 100 mg·kg-1), as well as their counterparts, bulk Fe3O4 particles, on the lettuce growth and the soil bacterial community. We not only measured the net photosynthetic rate of plant leaves, the Fe content in plant, but also determined the soil bacterial community structure and components using the high throughput technique. Results showed that responses of the plant and bacterial community varied with the increase of Fe3O4 nanoparticles concentration. At the low concentration, Fe3O4 nanoparticles stimulated plant growth, accompanied by the increased abundance of Xanthomonadales and the decreased abundance of Cyanobacteria and Sphingobacteria, but no significant changes were observed for the bacterial alpha diversity indices. At the high concentration, Fe3O4 nanoparticles could not only suppress plant growth, increase Fe content in plant tissue and soil electronic conductivity, but also decrease the phylogenetic diversity of bacterial community, reduce the abundances of Xanthomonadales and Sphingobacteria, and increase the proportion of Cyanobacteria. Moreover, the responses of some functional guilds in soil bacterial community varied between the Fe3O4 nanoparticle treatment and the bulk counterpart. This indicated that the size and the concentration of Fe3O4 were the factors influencing soil bacterial community, which would potentially impact the plant development. Therefore, more attention should be focused on soil microbes when evaluating the biological effects of nanoparticles.