Abstract: The effects of Bacillus Bs10, Ba12 and Bl10 on tomato growth and soil rhizosphere microorganisms were determined by Petri dish germination test and pot experiments. The results showed that Bs10, Ba12 and Bl10 exhibited remarkable promoting effect on the length of hypocotyl and radical, as well as the growth of plants. The length, surface area and volume of tomato roots increased significantly after treatment with Bacillus stains, the numbers and proportions of soil bacteria also increased markedly, while those of soil fungi decreased. The numbers of the dominant bacteria and fungi were changed, withBacillus methylotrophicus being much higher in root zone soil, surface soil and neouchi, while the plant pathogens Fusarium solani and Fusarium oxysporum decreased significantly in root zone soil and root surface soil. These results suggested that the addition of Bs10, Ba12 and Bl10 could improve the micro-ecosystem of the root domain of tomato, which might play an important role in growth promoting and disease prevention on tomato plants.