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应用生态学报 ›› 2018, Vol. 29 ›› Issue (2): 433-440.doi: 10.13287/j.1001-9332.201802.001

• 研究报告 • 上一篇    下一篇

温度对温带和亚热带森林土壤有机碳矿化速率及酶动力学参数的影响

刘霜1,2, 张心昱1,2*, 杨洋1,2, 唐玉倩1,2, 王忠强3   

  1. 1中国科学院地理科学与资源研究所生态系统网络观测与模拟重点实验室, 北京 100101;
    2中国科学院大学资源与环境学院, 北京 100190;
    3东北师范大学地理科学学院, 长春 130024
  • 收稿日期:2017-07-17 出版日期:2018-02-18 发布日期:2018-02-18
  • 通讯作者: E-mail: zhangxy@igsnrr.ac.cn
  • 作者简介:刘 霜, 女, 1995年生, 硕士研究生. 主要从事土壤碳循环的微生物机制研究. E-mail: lius.17s@igsnrr.ac.cn
  • 基金资助:

    本文由国家自然科学基金项目(41571251,41571130043)和中国科学院技术创新项目(201604)资助

Effects of temperature on soil organic carbon mineralization rate and enzyme kinetic para-meters in temperate and subtropical forests.

LIU Shuang1,2, ZHANG Xin-yu1,2*, YANG Yang1,2, TANG Yu-qian1,2, WANG Zhong-qiang3   

  1. 1Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;
    2College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100190, China;
    3School of Geographical Sciences, Northeast Normal University, Changchun 130024, China
  • Received:2017-07-17 Online:2018-02-18 Published:2018-02-18
  • Contact: E-mail: zhangxy@igsnrr.ac.cn
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (41571251, 41571130043) and the Technology Innovation Program of Chinese Academy of Sciences (201604).

摘要: 研究了温度对长白山阔叶红松林、鼎湖山常绿阔叶林2个不同纬度的森林土壤有机碳矿化速率和酶动力学参数的影响.结果表明: 土壤有机碳矿化速率(Cmin)随着温度的增加而增加,长白山土壤Cmin及其温度敏感性(Q10(Cmin))显著高于鼎湖山土壤.长白山土壤β-1,4-葡萄糖苷酶(βG)和β-1,4-N-乙酰葡糖氨糖苷酶(NAG)的酶动力学参数潜在最大反应速率(Vmax)和半饱和常数(Km)高于鼎湖山土壤,但鼎湖山土壤的催化效率(Vmax/Km)高于长白山土壤,表明随着温度的升高,土壤βG和NAG的VmaxVmax/Km增加,Km降低,即酶与底物的结合程度增加.鼎湖山土壤βG的Q10(Vmax)、Q10(Km)高于长白山土壤,这与土壤Q10(Cmin)结果不一致.增温对长白山和鼎湖山森林土壤有机碳矿化及酶动力学参数的影响机制不同,在土壤生物化学过程对增温响应的模型中应区别考虑.

Abstract: We examined the effects of temperature on SOC mineralization rate (Cmin) and enzyme kinetic parameters in soils of two different latitudinal forests, i.e., broad-leaved Korean pine forest in Changbai Mountain at higher latitude and evergreen broad-leaved forest in Dinghu Mountain at lower latitude. The results showed that the soil Cmin increased with temperature, and the Cmin and Q10(Cmin) of the Changbai Mountain forest soil were higher than those of the Dinghu Mountain forest soil. The maximal rates of velocity (Vmax) and the half-saturation constant (Km) of the Changbai Mountain forest soil were higher relative to the Dinghu Mountain soil for both soil β-glucosidase (βG) and N-acetyl-β-D-glucosaminidase (NAG), but being opposite for catalytic efficiency (Vmax/Km). Both Vmax and Vmax/Km of βG and NAG increased with the increase of incubation temperature, while Km decreased, i.e., the affinity of enzymes to the substrate were increased. Inconsistent with the soil Q10 (Cmin), Q10(Vmax) and Q10(Km) of the soil βG were higher in Dinghu Mountain forest soil than those in the Changbai Mountain forest soil. The mechanisms underlying the effects of temperate variation on Cmin and the enzyme kinetic parameters were different, and thus the temperature sensitivity of Cmin and enzyme kinetic parameters should be considered differentially in the models of soil biochemical process.