脱甲河水系CH4关键产生途径及其稳定碳同位素特征

doi:10.13287/j.1001-9332.201805.030

应用生态学报 ›› 2018, Vol. 29 ›› Issue (5): 1450-1460.doi: 10.13287/j.1001-9332.201805.030

• 稳定同位素生态学专栏 • 上一篇下一篇

脱甲河水系CH₄关键产生途径及其稳定碳同位素特征

赵强^1,2, 吕成文², 秦晓波^1*, 吴红宝¹, 万运帆¹, 廖育林³, 鲁艳红³, 王斌¹, 李勇⁴

¹中国农业科学院农业环境与可持续发展研究所/农业部农业环境重点实验室, 北京 100081;
²安徽师范大学国土资源与旅游学院, 安徽芜湖 241002;
³湖南省土壤肥料研究所, 长沙 410125;
⁴中国科学院亚热带农业生态研究所, 长沙 410125

收稿日期:2017-12-27 出版日期:2018-05-18 发布日期:2018-05-18
通讯作者: *E-mail: qinxiaobo@caas.cn
作者简介:赵强,男,1990年生,硕士研究生. 主要从事淡水生态系统温室气体排放和碳氮循环研究. E-mail: yqq19910309@163.com
基金资助:
本文由国家自然科学基金项目(41475129,41775157)和国家发改委CDM基金项目(2014081)资助

Key pathway of methane production and characteristics of stable carbon isotope of the Tuojia River waterbody.

ZHAO Qiang^1,2, LYU Cheng-wen², QIN Xiao-bo^1*, WU Hong-bao¹, WAN Yun-fan¹, LIAO Yu-lin³, LU Yan-hong³, WANG Bin¹, LI Yong⁴

¹Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agricultural Environment, Ministry of Agriculture, Beijing 100081, China;
²College of Territorial Resources and Tourism, Anhui Normal University, Wuhu 241002, Anhui, China;
³Hunan Institute of Soil and Fertilizer, Changsha 410125, China;
⁴Institute of Subtropical Agriculture, Chinese Aca-demy of Sciences, Changsha 410125, China

Received:2017-12-27 Online:2018-05-18 Published:2018-05-18
Contact: *E-mail: qinxiaobo@caas.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (41475129,41775157) and the National Development and Reform Commission Clean Development Mechanism Foundation (2014081)

摘要/Abstract

摘要： 为明确脱甲河溶存CH₄关键产生途径,明晰水系碳同位素组成及其分布特征,为小流域CH₄排放估算和减排提供数据支撑.利用双层扩散模型法估算了CH₄浓度和传输通量,研究了周年内脱甲河4级河段(S₁～S₄)水体CH₄通量的时空分布及其主控环境因子;运用稳定同位素方法探究了溶存CH₄关键产生途径,分析了溶解CH₄、悬浮颗粒物和沉积物有机质δ¹³C分布特征.结果表明: 水体pH均值为(7.27±0.03),各河段四季差异均显著;溶解氧(DO)在0.43～13.99 mg·L^-1内变化,S₁河段DO浓度最高且夏、秋季差异显著,其他河段均为冬与春、夏、秋季差异显著;可溶性有机碳(DOC)变化范围是0.34～8.32 mg·L^-1,由S₁至S₄河段总体呈递增趋势;水体电导率(EC)和氧化还原电位(ORP)变化范围分别是17～436 μS·cm^-1和-52.30～674.10 mV,各河段差异明显;铵态氮(NH₄⁺-N)、硝态氮(NO₃^--N)浓度分别在0.30～1.35(平均0.90±0.10) mg·L^-1和0.82～2.45 (平均1.62±0.16) mg·L^-1内变化.溶存CH₄浓度和传输通量变化范围分别是0～5.28 (平均0.46±0.06) μmol·L^-1和-0.34～619.72 (平均53.88±7.15) μg C·m^-2·h^-1;均存在时空变化且变异规律相似,为春季>冬季>夏季>秋季,S₂>S₃>S₄>S₁.通量与水体铵态氮和DOC浓度均呈显著正相关.各级河段均以乙酸发酵产甲烷途径为主导,但不同河段差异明显,乙酸发酵途径产CH₄贡献率以S₁河段最高(87%),其次为S₄(81%),S₂、S₃分别达到78%和76%.溶存CH₄、悬浮颗粒物和沉积物有机质的δ¹³C均值分别为-41.64‰±1.91‰、-14.07‰±1.06‰和-26.20‰±1.02‰,溶存甲烷δ¹³C与沉积物有机质的δ¹³C呈显著正相关,与其传输通量呈极显著负相关.

Abstract: This study aimed at exploring the key pathway of methane production and clarifying the composition and distribution of carbon (C) isotopes in the Tuojia River waterbody in Hunan Pro-vince. We estimated CH₄ concentrations and fluxes of four reaches (S₁, S₂, S₃ and S₄) by a two-layer diffusion model and gas chromatography. The spatial and temporal distribution of CH₄ flux and its relationship with environmental factors were examined. The key pathway of CH₄ production was investigated by stable C isotope method to analyze the distribution characteristics of ¹³C isotope (δ¹³C) of water dissolved CH₄ and seston/benthic organic matter. There was significant seasonal variability in water pH, with mean value of (7.27±0.03). The concentration of dissolved oxygen (DO) showed strong seasonal and spatial variations, with the range of 0.43-13.99 mg·L^-1. The maximum value of DO occurred in S₁ and differed significantly in summer and autumin. In addition, DO differed significantly in winter and other seasons in S₂, S₃ and S₄. The concentration of dissolved organic carbon (DOC) showed a gradual increasing trend from source to estuary. The highest concentration of DOC (8.32 mg·L^-1) was found in S₂, while the lowest was observed in S₁ (0.34 mg·L^-1). The electrical conductivity (EC) and oxidation-reduction potential (ORP) of water ranged from 17 to 436 μS·cm^-1 and from -52.30 to 674.10 mV, respectively, which were significantly different among the four reaches (P<0.05). Water ammonium nitrogen (NH₄⁺-N) and nitrate nitrogen (NO₃^--N) concentrations were in the ranges of 0.30-1.35 (averaged 0.90±0.10) mg·L^-1 and 0.82-2.45 (averaged 1.62±0.16) mg·L^-1, respectively. The dissolved concentration and diffusion flux of CH₄ ranged from 0 to 5.28 μmol·L^-1 and from -0.34 to 619.72 μg C·m^-2·h^-1, respectively, with significant temporal and spatial variations. They showed a similar trend among reaches. Their values were highest in spring, followed by in winter and lowest in summer and autumn. Spatially, the CH₄ concentration and flux followed the order of S₂>S₃>S₄>S₁. The correlation analysis showed that CH₄ flux was positively correlated with NH₄⁺-N and DOC. The pathway of CH₄ production of all reaches was dominated by acetic acid fermentation, while there were obvious differences among the four reaches. The contribution of CH₄ from acetic acid fermentation was greatest (87%) in S₁, followed by S₄(81%), S₂(78%) and S₃(76%). The mean value of the δ¹³C for dissolved CH₄, seston organic matter and benthic organic matter was -41.64‰±1.91‰, -14.07‰±1.06‰ and -26.20‰±1.02‰, respectively. There was a positive correlation between the δ¹³C of dissolved CH₄ and benthic organic matter, whereas the δ¹³C value of dissolved CH₄was negatively correlated with CH₄flux.

赵强, 吕成文, 秦晓波, 吴红宝, 万运帆, 廖育林, 鲁艳红, 王斌, 李勇. 脱甲河水系CH₄关键产生途径及其稳定碳同位素特征[J]. 应用生态学报, 2018, 29(5): 1450-1460.

ZHAO Qiang, LYU Cheng-wen, QIN Xiao-bo, WU Hong-bao, WAN Yun-fan, LIAO Yu-lin, LU Yan-hong, WANG Bin, LI Yong. Key pathway of methane production and characteristics of stable carbon isotope of the Tuojia River waterbody.[J]. Chinese Journal of Applied Ecology, 2018, 29(5): 1450-1460.

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脱甲河水系CH₄关键产生途径及其稳定碳同位素特征

Key pathway of methane production and characteristics of stable carbon isotope of the Tuojia River waterbody.

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