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应用生态学报 ›› 2018, Vol. 29 ›› Issue (6): 1969-1974.doi: 10.13287/j.1001-9332.201806.039

• 研究报告 • 上一篇    下一篇

Cd胁迫对舞毒蛾幼虫食物利用的影响及其Cd的外泌机制

姜礅,狄贵秋,严善春*   

  1. 东北林业大学林学院, 哈尔滨 150040
  • 收稿日期:2017-10-03 修回日期:2018-03-27 出版日期:2018-06-18 发布日期:2018-06-18
  • 通讯作者: E-mail: yanshanchun@126.com
  • 作者简介:姜礅, 男, 1993年生, 博士研究生. 主要从事昆虫化学生态研究. E-mail: 821880041@qq.com
  • 基金资助:

    本文由东北林业大学学术名师支持计划项目(010602071)资助

Food utilization and excretion mechanism of Cd in gypsy moth larvae fed on Cd-enriched Populus alba×P. berolinensis leaves

JIANG Dun, DI Gui-qiu, YAN Shan-chun*   

  1. College of Forestry, Northeast Forestry University, Harbin 150040, China
  • Received:2017-10-03 Revised:2018-03-27 Online:2018-06-18 Published:2018-06-18
  • Supported by:

    This work was supported by the Program for Famous Teachers of Northeast Forestry University (010602071)

摘要: 为探明植食性昆虫对受重金属胁迫的寄主植物的生理生态响应机制,本研究用Cd胁迫下银中杨的叶片饲养舞毒蛾幼虫,分析舞毒蛾幼虫对食物的利用情况以及其对Cd的排毒代谢机制.结果表明: 取食Cd胁迫下银中杨的叶片后,舞毒蛾3~6龄幼虫体内的Cd浓度和Cd含量均显著高于对照,但随着幼虫龄期增长,其体内Cd浓度显著降低,而Cd含量有不同程度的提高;舞毒蛾幼虫粪便和虫蜕中的Cd浓度均显著高于对照;舞毒蛾3~5龄幼虫的食物消耗率显著高于对照,而转化率显著低于对照;3~4龄幼虫的食物利用率均与对照差异不显著,但在5龄时显著低于对照.说明在Cd胁迫下,舞毒蛾幼虫能通过有效的排毒代谢途径将体内富集的部分Cd排出体外,且高龄幼虫的排毒代谢能力强于低龄幼虫;舞毒蛾幼虫体质量的增加会对体内的Cd浓度形成一种稀释效应;舞毒蛾幼虫能通过调整食物消耗率和转化率之间的比例,来维持其正常生长发育所需的食物利用率,但超过一定限度后仍会造成食物利用率降低.

Abstract: To investigate the eco-physiological responses of phytophagous insects to host plants exposed to heavy metal stress, the gypsy moth (Lymantria dispar) larvae were reared with leaves of Populus alba×P. berolinensis grown in Cd-contaminated soil to analyze their food utilization and mechanisms underlying Cd excretion. Our results showed that after feeding on Cd-stressed poplar leaves, Cd concentrations and contents in the 3rd-6th instar larvae were significantly higher than those in control. The Cd concentrations significantly decreased with the larval age, whereas it was reverse for Cd contents. Cd concentrations in the larval faces and exuvia were significantly higher than those in control. Food digestion/consumption rate and conversion rate of the 3rd-5th instar larvae in the Cd-treatment group were significantly higher and lower than those in the control, respectively. Food utilization rate of the Cd-stressed 3rd-4th instar larvae were not significantly different from that of the control larvae with the same age. In contrast, the Cd-stressed 5th instar larvae showed a significantly lower food utilization rate than the control. These results indicated that gypsy moth larvae could remove some Cd from their bodies through effective excretion (e.g. feces and exuvia), with the mature larvae being more effective than the juveniles. The increases of larval body mass seemed to have a “dilution effect” on its Cd concentration. Under Cd stress, the gypsy moth larvae might maintain a certain level of food utilization rate for growth and development via a modulation of food consumption/conversion rates, but Cd-stress would still reduce the larval food utilization rate when it was beyond a certain threshold.