碳氮添加对草地土壤有机碳氮磷含量及相关酶活性的影响

doi:10.13287/j.1001-9332.201808.031

应用生态学报 ›› 2018, Vol. 29 ›› Issue (8): 2470-2476.doi: 10.13287/j.1001-9332.201808.031

碳氮添加对草地土壤有机碳氮磷含量及相关酶活性的影响

李焕茹^1,2,3*, 朱莹^1,2, 田纪辉⁴, 魏锴¹, 陈振华¹, 陈利军^1*

¹中国科学院沈阳应用生态研究所, 沈阳 110016;
²中国科学院大学, 北京 100049;
³中国科学院沈阳应用生态研究所, 额尔古纳森林草原过渡带生态系统研究站, 沈阳 110016;
⁴华南农业大学, 广州 510642

收稿日期:2017-10-30 出版日期:2018-08-20 发布日期:2018-08-20
通讯作者: E-mail: ljchen@iae.ac.cn; ljchenchina@hotmail.com
作者简介:李焕茹, 女,1991年生, 硕士研究生. 主要从事草地生态研究. E-mail: lihuanruaaa@163.com
基金资助:
本文由国家自然科学基金项目(41771333, 41701344)资助

Effects of carbon and nitrogen additions on soil organic C, N, P contents and their catalyzed enzyme activities in a grassland.

LI Huan-ru^1,2,3, ZHU Ying^1,2, TIAN Ji-hui⁴, WEI Kai¹, CHEN Zhen-hua¹, CHEN Li-jun^1*

¹Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
²University of Chinese Academy of Sciences, Beijing 100049, China;
³Erguna Forest-Steppe Ecotone Research Station, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
⁴South China Agricultural University, Guangzhou 510642, China.

Received:2017-10-30 Online:2018-08-20 Published:2018-08-20
Supported by:
This work was supported by the National Natural Science Foundation of China (41771333, 41701344)

摘要/Abstract

摘要： 草地土壤有机碳(C)、氮(N)、磷(P)等养分含量和酶活性对草地生态系统能量和养分的保持和供应具有重要作用.氮沉降对草地生态系统土壤有机养分及酶活性产生影响的结果不一致性,碳的同步添加是否会缓解氮沉降造成的负面影响仍不清楚,需要深入探讨.本研究以在内蒙古呼伦贝尔草原开展的碳(葡萄糖)、氮(尿素)添加试验样地为依托(始于2014年5月),探讨碳、氮添加对草地土壤C、N、P含量及相关酶活性的影响及其机制.试验分别设N₀(对照)、N₂₅(施氮25 kg·hm^-2·a^-1)、N₅₀(50 kg·hm^-2·a^-1)、N₁₀₀(100 kg·hm^-2·a^-1)、N₂₀₀(200 kg·hm^-2·a^-1)共5个N添加主处理,C₀(对照)、C₂₅₀(施碳250 kg·hm^-2·a^-1)、C₅₀₀(500 kg·hm^-2·a^-1)3个碳添加副处理,试验样品采于2016年8月.结果表明:高氮添加显著抑制脱氢酶(DHA)和β-1,4-N-乙酰氨基葡糖苷酶(NAG)活性,与对照相比,其活性分别降低22.3%和12.5%;而氮添加对土壤有机N含量无显著影响,使有机C和有机P含量分别减少6.6%和14.5%.高碳添加缓解了土壤微生物的碳限制,使得脱氢酶(DHA)、β-葡糖苷酶(BG)活性及土壤有机N、有机P含量分别增加15.1%、12.2%、1.9%、2.6%.研究表明,长期过量氮输入抑制土壤微生物活性,造成土壤有机C、有机P的减少,而碳添加提高了微生物及酶活性,使土壤有机N、P含量增加.碳氮耦合添加对草地土壤有机C、N、P的持续供应具有重要意义.

Abstract: Soil organic C, N, P contents and their catalyzed enzyme activities play an important role in maintaining and supplying energy and nutrient in grasslands. There is no consensus on the effects of N deposition on soil organic nutrients and enzyme activities in grassland ecosystems. It remains unclear whether C addition will retard the negative effects of N deposition. We carried out an experiment in Hulun Buir grassland of Inner Mongolia to examine the effects of C and N additions on soil organic C, N, P and relative enzyme activities after three years treatments. The experiment was conducted with N treatments at five levels (0, 25, 50, 100 and 200 kg·hm^-2·a^-1) and with C treatments at three levels (0, 250 and 500 kg·hm^-2·a^-1). The results showed that higher levels of N addition significantly decreased dehydrogenase (DHA) and β-1,4-N-acetylglucosaminidase (NAG) activities by 22.3% and 12.5%, respectively. Nitrogen addition had no significant effect on soil organic N and decreased the organic C and P contents by 6.6% and 14.5%, respectively. High C addition significantly increased DHA, β-glucosidase (BG) activities and increased soil organic N and organic P by 15.1%, 12.2%, 1.9%, 2.6%, respectively. The results suggested that continuous N inputs inhibited microbial activities and caused losses of soil organic C and organic P. Carbon addition could enhance microbial activities and promote the secretion of enzymes and increase soil organic N and P. The combined C and N addition could play an important role in maintaining the balance and supply of soil C, N, and P in grassland ecosystem.

李焕茹, 朱莹, 田纪辉, 魏锴, 陈振华, 陈利军. 碳氮添加对草地土壤有机碳氮磷含量及相关酶活性的影响[J]. 应用生态学报, 2018, 29(8): 2470-2476.

LI Huan-ru, ZHU Ying, TIAN Ji-hui, WEI Kai, CHEN Zhen-hua, CHEN Li-jun. Effects of carbon and nitrogen additions on soil organic C, N, P contents and their catalyzed enzyme activities in a grassland.[J]. Chinese Journal of Applied Ecology, 2018, 29(8): 2470-2476.

参考文献

[1] Department of Animal Husbandry and Veterinary, General Station of Animal Husbandry and Veterinary of Ministry of Agriculture of China (中华人民共和国农业部畜牧兽医司,全国畜牧兽医总站). Rangeland Resources of China. Beijing: China Science and Technology Press, 1996 (in Chinese)
[2] Shen H-H (沈海花), Zhu Y-K (朱言坤), Zhao X (赵霞), et al. Analysis of current grassland resources in China. Chinese Science Bulletin (科学通报), 2016, 61(2): 139-154 (in chinese)
[3] Delgado-Baquerizo M, Maestre FT, Gallardo A, et al. Decoupling of soil nutrient cycles as a function of aridity in global drylands. Nature, 2013, 502: 672-676
[4] Long M, Wu HH, Smith MD, et al. Nitrogen deposition promotes phosphorus uptake of plants in a semi-arid temperate grassland. Plant and Soil, 2016, 408: 475-484
[5] Lebauer DS, Treseder KK. Nitrogen limitation of net primary productivity in terrestrial ecosystems is globally distributed. Ecology, 2008, 89: 371-379
[6] Woodmansee RG, Dodd JL, Bowman RA, et al. Nitrogen budget of a shortgrass prairie ecosystem. Oecologia, 1978, 34: 363-376
[7] Zhang B-G (张宝贵), Li G-T (李贵桐). Roles of soil organisms on the enhancement of plant availability of phosphorus. Acta Pedologica Sinica (土壤学报), 1998, 35(1): 104-111 (in Chinese)
[8] Zhou L-K (周礼恺). Soil Enzymology. Beijing: Science Press, 1987: 116-206 (in Chinese)
[9] Liu X, Ying Z, Han W, et al. Enhanced nitrogen deposition over China. Nature, 2013, 494: 459-462
[10] Shi Y, Sheng L, Wang Z, et al. Responses of soil enzyme activity and microbial community compositions to nitrogen addition in bulk and microaggregate soil in the temperate steppe of Inner Mongolia. Eurasian Soil Science, 2016, 49: 1149-1160
[11] Malhi SS, Harapiak JT, Nyborg M, et al. Total and light fraction organic C in a thin Black Chernozemic grassland soil as affected by 27 annual applications of six rates of fertilizer N. Nutrient Cycling in Agroecosystems, 2003, 66: 33-41
[12] Turner BL, Chudek JA, Whitton BR. Phosphorus composition of upland soils polluted by long-term atmosphe-ric nitrogen deposition. Biogeochemistry, 2003, 65: 259-274
[13] Mack MC, Schuur EA, Bretharte MS, et al. Ecosystem carbon storage in arctic tundra reduced by long-term nutrient fertilization. Nature, 2004, 431: 440-443
[14] Neff JC, Townsend AR, Gleixner G, et al. Variable effects of nitrogen additions on the stability and turnover of soil carbon. Nature, 2002, 419: 915-917
[15] Tian J, Wei K, Condron LM, et al. Impact of land use and nutrient addition on phosphatase activities and their relationships with organic phosphorus turnover in semi-arid grassland soils. Biology and Fertility of Soils, 2016, 52: 675-683
[16] Wang R, Filley TR, Xu Z, et al. Coupled response of soil carbon and nitrogen pools and enzyme activities to nitrogen and water addition in a semi-arid grassland of Inner Mongolia. Plant and Soil, 2014, 381: 323-336
[17] Hassink J, Neeteson JJ. Effect of grassland management on the amounts of soil organic N and C. The Netherlands Journal of Agricultural Science, 1991, 39: 225-236
[18] Yang S (杨山). Effects of Water and Fertilizer Addition on Soil Microbial Characteristics in a Typical Grassland of Inner Mongolia. PhD Thesis. Shenyang: Shen-yang University, 2014 (in Chinese)
[19] Yu Y-Y (于跃跃), Zhao B-Z (赵炳梓). Effect of amendment inorganic nitrogen and glucose on soil microbial biomass and activity. Acta Pedologica Sinica (土壤学报), 2012, 49(1): 139-146 (in Chinese)
[20] Xu L-L (徐丽丽), Wang Q-B (王秋兵), Zhang X-Y (张心昱), et al. Effects of applying different kind fertilizers on enzyme activities related to carbon, nitrogen, and phosphorus cycles in reddish paddy soil. Chinese Journal of Applied Ecology (应用生态学报), 2013, 24(4): 909-914 (in Chinese)
[21] Jia J-X (贾俊仙), Li Z-P (李忠佩), Che Y-P (车玉萍). Effects of glucose addition on N transformations in paddy soils with a gradient of organic C content in subtropical China. Scientia Agricultura Sinica (中国农业科学), 2010, 43(8): 1617-1624 (in Chinese)
[22] Zhang L (张乐), He H-B (何红波), Zhang J-X (章建新), et al. Effect of glucose addtion with different amount on extraneous nitrogen transformation in soil. Chinese Journal of Soil Science (土壤通报), 2008, 39(4): 775-778 (in Chinese)
[23] Lu R-K (鲁如坤). Soil and Agro-chemistry Analysis. Beijing: China Agricultural Science and Technology Press, 2000 (in Chinese)
[24] Kuo S. Phosphorus// Sparks DL, Page AL, Helmke PA, eds. Methods of Soil Analysis. Part 3: Chemical Methods. Madison, WI,USA: Soil Science Society of America, 1996: 869-919
[25] Tabatabai MA. Soil enzymes// Sparks DL, Page AL, Helmke PA, eds. Methods of Soil Analysis. Part 2: Microbiological and Biochemical Properties. Madison, WI,USA: Soil Science Society of America,1994: 775-834
[26] Tian D, Niu S. A global analysis of soil acidification caused by nitrogen addition. Environmental Research Letters, 2015, 10: 2, doi: 10.1088/1748-9326/10/2/024019
[27] Matschonat G, Matzner E. Soil chemical properties affecting NH₄⁺ sorption in forest soils. Journal of Plant Nutrition and Soil Science, 2015, 159: 505-511
[28] Keeler BL, Hobbie SE, Kellogg LE. Effects of long-term nitrogen addition on microbial enzyme activity in eight forested and grassland sites: Implications for litter and soil organic matter decomposition. Ecosystems, 2009, 12: 1-15
[29] Ning Q-S (宁秋实). Effects of Nitrogen Addition on Soil Microbial Processes in Temperate Grassland in Inner Mongolia. PhD Thesis. Beijing: Chinese Academy of Sciences, 2016 (in Chinese)
[30] Dick WA, Cheng L, Wang P. Soil acid and alkaline phosphatase activity as pH adjustment indicators. Soil Biology and Biochemistry, 2000, 32: 1915-1919
[31] Wang H (王涵), Wang G (王果), Huang Y-Y (黄颖颖), et al. The effects of pH change on the acti-vities of enzymes in acid soil. Ecology and Environment (生态环境学报), 2008, 17(6): 2401-2406 (in Chinese)
[32] Simpson AJ, Simpson MJ, Smith E, et al. Microbially derived inputs to soil organic matter: Are current estimates too low? Environmental Science and Technology, 2007, 41: 8070-8076
[33] Geisseler D, Lazicki PA, Scow KM. Mineral nitrogen input decreases microbial biomass in soils under grasslands but not annual crops. Applied Soil Ecology, 2016, 106: 1-10

碳氮添加对草地土壤有机碳氮磷含量及相关酶活性的影响

Effects of carbon and nitrogen additions on soil organic C, N, P contents and their catalyzed enzyme activities in a grassland.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价