Abstract: Urban green land is an important component of urban natural ecosystems, with critical ecosystem service functions. Based on the land use data of Shenyang in 2005, 2010 and 2015, this study estimated ecosystem service value (ESV) according to “equivalent value per unit area of ecosystem services for various ecosystems in China” and then analyzed its spatio-temporal characteristics and change rate. The results showed that arable land area significantly reduced by 3.5% from 2005 to 2010 and 10.9% from 2010 to 2015, respectively. Wood land area increased slightly, and the area of meadow increased by 17.2%. The ESV of green land in Shenyang increased from 11.76×10⁸ yuan in 2005 to 23.48×10⁸ yuan in 2010, and then decreased to 11.29×10⁸ yuan in 2015. The annual change of ESV showed significant difference among different districts, with the highest occurring in Yuhong, Shenbei and Heping districts. The coefficient of variation (CV) was 64.9%, 60.4% and 40.8%, respectively. The ESV of green land varied significantly among diffe-rent districts, with higher values occurring in Huanggu, Shenbei and Dongling districts. Soil conservation function had the highest ESV, accounting for 15% of the total ESV. The food production function had the lowest ESV. The changes of ESV in Shenyang might be attributed to the urban expansion, agricultural land conversion, and green space construction.