桉树油化学成分分析及α-松油醇的化感作用

doi:10.13287/j.1001-9332.202007.002

应用生态学报 ›› 2020, Vol. 31 ›› Issue (7): 2195-2201.doi: 10.13287/j.1001-9332.202007.002

桉树油化学成分分析及α-松油醇的化感作用

李奥欣¹, 侯新村², 曾加佳¹, 魏芸^1*

¹北京化工大学化工资源有效利用国家重点实验室, 北京 100029;
²北京市农林科学院草业与环境研究发展中心, 北京 100097

收稿日期:2020-01-01 接受日期:2020-03-10 出版日期:2020-07-15 发布日期:2021-01-15
通讯作者: E-mail: weiyun@mail.buct.edu.cn
作者简介:李奥欣, 女, 1992年生, 博士研究生。主要从事天然产物分析及活性研究。E-mail: for_chem@163.com
基金资助:
国家科技重大专项(2019ZX09301-114)资助

Chemical composition analysis of Eucalyptus essential oil and allelopathic effects of α-terpineol

LI Ao-xin¹, HOU Xin-cun², ZENG Jia-jia¹, WEI Yun^1*

¹State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China;
²Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received:2020-01-01 Accepted:2020-03-10 Online:2020-07-15 Published:2021-01-15
Contact: E-mail: weiyun@mail.buct.edu.cn
Supported by:
This work was supported by the National Science and Technology Major Project (2019ZX09301-114).

摘要/Abstract

摘要： 采用水蒸气蒸馏法提取桉树油,通过GC-MS对其成分进行分析,以其中一种化学成分α-松油醇为代表,分别采用培养皿和盆栽试验研究其对反枝苋的化感作用。结果表明: 水蒸气蒸馏法提取桉树油的产率为0.04%,经GC-MS分析,在桉树油中共检测到92种化学成分。在培养皿条件下,α-松油醇对反枝苋的发芽和生长均具有明显的抑制作用,用量达到每皿5 和7.5 μL时,抑制率均达到100%。盆栽条件下,α-松油醇处理过的土壤会显著影响反枝苋的出苗率、地上鲜重和地上干重,浓度达到1.6 μL·cm^-3时,抑制作用最大,其化感响应指数分别为-0.51、-0.62和-0.44,抑制率为51%、62%和44%。

关键词: 桉树, 反枝苋, 挥发油, α-松油醇

Abstract: We extracted Eucalyptus essential oil by steam distillation and analyzed its chemical components by GC-MS. The inhibitory effects of α-terpineol, a component in Eucalyptus essential oil, were tested in Petri dishes on the germination and growth of Amaranthus retroflexus. The allelopathic effects of α-terpineol on A. retroflexus were examined with a pot experiment by measuring germination rate and biomass. The yield of essential oil was 0.04%. Ninety-two chemical components were detected from the essential oil. In Petri dish experiment, the germination inhibitory effect under 5 μL and 7.5 μL of α-terpineol per dish reached 100%. In pot experiment, germination, aboveground fresh and dry weights of A. retroflexus were significantly affected by α-terpineol. At the concentration of 1.6 μL·cm^-3, the inhibition effect was strongest, with the allelopathic response index being -0.51 for germination, -0.62 for aboveground fresh weight, and -0.44 for aboveground dry weight, and the inhibition ratio being 51%, 62% and 44%, respectively.

Key words: Eucalyptus, Amaranthus retroflexus, essential oil, α-terpineol

李奥欣, 侯新村, 曾加佳, 魏芸. 桉树油化学成分分析及α-松油醇的化感作用[J]. 应用生态学报, 2020, 31(7): 2195-2201.

LI Ao-xin, HOU Xin-cun, ZENG Jia-jia, WEI Yun. Chemical composition analysis of Eucalyptus essential oil and allelopathic effects of α-terpineol[J]. Chinese Journal of Applied Ecology, 2020, 31(7): 2195-2201.

参考文献

[1] 林政. 我国桉树专题文献研究. 农业图书情报学刊, 2006, 18(1): 152-154 [Lin Z. Bibliographc study on the subject of Eucalyptus in China. Journal of Library and Information Sciences of Agriculture, 2006, 18(1): 152-154]
[2] 黄国勤, 赵其国. 广西桉树种植的历史、现状、生态问题及应对策略. 生态学报, 2014, 34(18): 5142-5152 [Huang G-Q, Zhao Q-G. The history, status quo, ecological problems and countermeasures of Eucalyptus plantations in Guangxi. Acta Ecologica Sinica, 2014, 34(18): 5142-5152]
[3] 项东云, 郑白, 周维, 等. 广西桉树育种研究概述. 广西林业科学, 1999, 28(2): 71-91 [Xiang D-Y, Zheng B, Zhou W, et al. Overview of breeding research of Eucalyptus plantation in Guangxi. Guangxi Forestry Science, 1999, 28(2): 71-91]
[4] 李萃邦, 徐胜臻, 徐文兴, 等. 桉树叶活性成分提取方法及生物活性研究进展. 湖北农业科学, 2018, 57(6): 12-25 [Li C-B, Xu S-Z, Xu W-X, et al. Research progress on extraction methods and biological activities of active ingredients from Eucalyptus leaves. Hubei Agricultural Science, 2018, 57(6): 12-25]
[5] 李丽丽, 孙泽宇, 刘雄民, 等. 柠檬桉树脂总黄酮的提取纯化及抗氧化活性. 精细化工, 2019, 36(8): 1571-1577 [Li L-L, Sun Z-Y, Liu X-M, et al. Extraction, purification and antioxidant activities of total flavonoids in Eucalyptus citriodira Resin. Modern Food Science and Technology, 2019, 36(8): 1571-1577]
[6] 单体江, 冯皓, 谢银燕, 等. 柠檬桉内生真菌的分离及其提取物的抗细菌活性. 植物保护, 2019, 45(6): 149-155 [Shan T-J, Feng H, Xie Y-Y, et al. Endophytic fungi isolated from Eucalyptus citriodora Hook. f. and the antibacterial activity of their crude extracts. Plant Protection, 2019, 45(6): 149-155]
[7] 李佳雨, 林家怡, 裴晨羽, 等. 桉树种植对林地土壤丛枝菌根真菌群落结构及多样性的影响. 生态学报, 2019, 39(8): 2723-2731 [Li J-Y, Lin J-Y, Pei C-Y, et al. Diversity and structure of the soil arbuscular mycorrhizal fungal community are altered by Eucalyptus plantations. Acta Ecologica Sinica, 2019, 39(8): 2723-2731]
[8] 余俊里, 张健, 汤智群, 等. 一个年龄序列巨桉人工林土壤对赤子爱胜蚓生长和生理指标的影响. 生态学杂志, 2017, 36(1): 124-131 [Yu J-L, Zhang J, Tang Z-Q, et al. Variations of growth and physiological indicators of Eisenia foetida in soils across a chronosequence of Eucalyptus grandis plantations. Chinese Journal of Ecology, 2017, 36(1): 124-131]
[9] Zhang J, An M, Wu H, et al. Chemical composition of essential oils of four Eucalyptus species and their phytotoxicity on silverleaf nightshade (Solanum elaeagnifolium Cav.) in Australia. Plant Growth Regulation, 2012, 68: 231-237
[10] 郭峰, 黄玲, 曹子林, 等. 蓝桉枝叶四级温度梯度水提液对其种子的化感作用. 种子, 2013, 11(32): 42-45 [Guo F, Huang L, Cao Z-L, et al. Allelopathy of Eucalyptus globulus foliage of four level temperature water extract of Eucalyptus globulus seeds. Seed, 2013, 11(32): 42-45]
[11] 王静, 王磊, 李永青, 等. 3种桉树叶提取物除草活性研究. 中国农学通报, 2013, 29(6): 191-194 [Wang J, Wang L, Li Y-Q, et al. Study on herbicidal activity of three Eucalyptus leaf extracts. Chinese Agricultural Science Bulletin, 2013, 29(6): 191-194]
[12] Li A, Wu H, Feng Y, et al. A strategy of rapidly screening out herbicidal chemicals from Eucalyptus essential oils. Pest Management Science, 2020, 76: 917-927
[13] Martino LD, Mancini E, Almeida LF, et al. The antigerminative activity of twenty-seven monoterpenes. Molecules, 2010, 15: 6630-6637
[14] 夏虹, 黄新会, 何勇, 等. 桉树中5种化学成分对紫茎泽兰种子萌发和幼苗生长的影响. 云南农业大学学报: 自然科学, 2013, 28(3): 386-391 [Xia H, Huang X-H, He Y, et al. Effects of five important chemical compositions from Eucalyptus spp. on seed germination and seeding growth of Ageratina adenophora. Journal of Yunnan Agricultural University: Natural Science, 2013, 28(3): 386-391]
[15] 平晓燕, 王铁梅. 植物化感作用的生态学意义及在草地生态系统中的研究进展. 草业学报, 2018, 27(8): 175-184 [Ping X-Y, Wang T-M. Ecological significance of plant allelopathy and progress in allelopathy research in grassland ecosystems. Acta Prataculturae Sinica, 2018, 27(8): 175-184]
[16] 周泽建, 刘妮妮, 伍冰倩, 等. 3种速生树种落叶水浸提液对走马胎幼苗生长的化感效应. 植物研究, 2018, 38(4): 568-574 [Zhou Z-J, Liu N-N, Wu B-Q, et al. Allelopathic effects of leaves extracts on growth of three fast-growing tree species on Ardisia gigantifolia Staf seedlings. Bulletin of Botanical Research, 2018, 38(4): 568-574]
[17] 王小雪, 刘芸, 邵呈龙, 等. 5种经济植物对幼龄尾巨桉叶片提取液的化感敏感性. 林业科学, 2011, 47(11): 188-193 [Wang X-X, Liu Y, Shao C-L, et al. Allelopathic sensitivity of five economic species to aqueous leaf extract of Eucalyptus urophylla × E. grandis with different ages. Scientia Silvae Sinicae, 2011, 47(11): 188-193]
[18] 陈博雯, 刘海龙, 蔡玲, 等. 4种经济树种叶片浸提液对作物种子萌发化感作用的研究. 西部林业科学, 2012, 41(3): 31-36 [Chen B-W, Liu H-L, Cai L, et al. Bioassay of allelopathy of aqueous extracts of four main economic tree species on crop seeds. Journal of West China Forestry Science, 2012, 41(3): 31-36]
[19] 郑立浪. 尾巨桉叶化感效应与土壤微生物的相互影响. 硕士论文. 桂林: 广西师范大学, 2016 [Zheng L-L. Study on Mutual Effect between the Allelopathy of Eucalyptus urophylla × Eucalyptus grandis Leaves and Soil Microbe. Master Thesis. Guilin: Guangxi Normal University, 2016]
[20] Adams RP. Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectrometry. 4th Ed. Amsterdam, the Netherlands: Elesvier, 2007
[21] Bruce WG, Richardson D. Bioassays for allelopathy: Measuring treatment responses with independent controls. Journal of Chemical Ecology, 1988, 14: 181-187
[22] 沈慧敏, 郭鸿儒, 黄高宝. 不同植物对小麦、黄瓜和萝卜幼苗化感作用潜力的初步评价. 应用生态学报, 2005, 16(4): 740-743 [Shen H-M, Guo H-R, Huang G-B. Allelopathy of different plants on wheat, cucumber and radish seedlings. Chinese Journal of Applied Ecology, 2005, 16(4): 740-743]
[23] 陈虹霞, 王成章. 4种非洲桉树叶挥发油的化学成分研究. 生物质化学工程, 2010, 44(6): 23-27 [Chen H-X, Wang C-Z. Chemical compositions of essential oil of four Eucalyptus leaves from Africa. Biomass Chemical Engineering, 2010, 44(6): 23-27]
[24] 马丽, 蓝亮美, 郭占京, 等. 两种桉叶挥发油含量和化学成分周年变化. 精细化工, 2015, 32(3): 300-303 [Ma L, Lan L-M, Guo Z-J, et al. Annual change of contents and chemical compositions of essential oils from leaves of E. exserta and E. urophylla. Fine Chemicals, 2015, 32(3): 300-303]
[25] 孔垂华, 胡飞. 植物化感(相生相克)作用及其应用. 北京: 中国农业出版社, 2001 [Kong C-H, Hu F. Plant Allelopathy and Its Application. Beijing: China Agriculture Press, 2001]
[26] 孔垂华. 作物化感品种对农田杂草的调控. 植物保护学报, 2018, 45(5): 961-970 [Kong C-H. Allelopathic crop cultivars for weed management in cropping systems. Journal of Plant Protection, 2018, 45(5): 961-970]
[27] 王朋, 梁文举, 孔垂华, 等. 外来杂草入侵的化学机制. 应用生态学报, 2004, 15(4): 707-711 [Wang P, Liang W-J, Kong C-H, et al. Chemical mechanism of exotic weed invasion. Chinese Journal of Applied Eco-logy, 2004, 15(4): 707-711]
[28] Rice EL. Allelopathy. 2nd Ed. Orlando, FL, USA: Academic Press, 1984
[29] Batish DR, Singh HP, Kohli RK, et al. Eucalyptus essential oil as a natural pesticide. Forest Ecology and Management, 2008, 256: 2166-2174

桉树油化学成分分析及α-松油醇的化感作用

Chemical composition analysis of Eucalyptus essential oil and allelopathic effects of α-terpineol

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	舒成博, 沈影利, 刘刚, 张琼, 徐敬华, 郭珍. 南方山区典型小流域桉树人工林种植对水土流失的影响 [J]. 应用生态学报, 2023, 34(4): 1015-1023.
[2]	李春英, 田瑶, 于美婷, 赵春建. 苘麻挥发油对小麦、玉米和大豆萌发及幼苗生长的化感作用 [J]. 应用生态学报, 2020, 31(7): 2251-2256.
[3]	赵芸, 杨云海, 王凯博, 范黎明, 苏发武, 叶敏. 牛至挥发油的化学成分及其化感作用 [J]. 应用生态学报, 2020, 31(7): 2257-2263.
[4]	华雷,何茜**,李吉跃,刘珊,余斐. 桉树无性系和华南乡土树种秋枫苗木耗水特性的比较 [J]. 应用生态学报, 2014, 25(6): 1639-1644.
[5]	杨钙仁,张秀清,蔡德所,石贤辉,张华,黄承标. 广西主要人工林凋落物分解过程及其对淋溶水质的影响 [J]. 应用生态学报, 2012, 23(01): 9-16.
[6]	黄京华,谭钜发,揭红科,曾任森. 丛枝菌根真菌对黄花蒿生长及药效成分的影响 [J]. 应用生态学报, 2011, 22(06): 1443-1449.
[7]	马强,宇万太,周桦,徐永刚,陈进宁,陈桂金,刘淑英,邓力. 追施氮肥对桉树各器官养分浓度及贮量的影响 [J]. 应用生态学报, 2010, 21(08): 1933-1939.
[8]	刘姝媛,刘月秀,叶金盛,宫彦章,曾曙才. 广东省桉树人工林土壤有机碳密度及其影响因子 [J]. 应用生态学报, 2010, 21(08): 1981-1985.
[9]	王宇;戴传超;陈晏. 茅苍术挥发油对三种内生真菌及七种外源真菌的抑菌活性 [J]. 应用生态学报, 2009, 20(11): 2778-2784.
[10]	杨曾奖;徐大平;陈文平;黄烈健;李尚均;陈源. 华南地区桉树/相思混交种植的林木生长效应 [J]. 应用生态学报, 2009, 20(10): 2339-2344.
[11]	平亮^1,2;谢宗强¹. 引种桉树对本地生物多样性的影响 [J]. 应用生态学报, 2009, 20(07): 1765-1774 .
[12]	周天^1,2;郭继勋¹;韩德复^1,2;邢福¹;田尚衣¹. 黄蒿挥发油对蚊虫的毒杀活性及其化学成分 [J]. 应用生态学报, 2006, 17(05): 907-910 .
[13]	温远光, 刘世荣, 陈放. 连栽对桉树人工林下物种多样性的影响 [J]. 应用生态学报, 2005, 16(9): 1667-1671.
[14]	温远光^1，2 刘世荣³ 陈放¹. 连栽对桉树人工林下物种多样性的影响 [J]. 应用生态学报, 2005, 16(09): 1667-1671 .
[15]	周琼, 梁广文, 孔垂华, 黄寿山. 白蝴蝶挥发油对蚜虫的驱避作用及其化学成分 [J]. 应用生态学报, 2004, (5): 856-858.