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应用生态学报 ›› 2021, Vol. 32 ›› Issue (4): 1479-1488.doi: 10.13287/j.1001-9332.202104.037

• 研究论文 • 上一篇    下一篇

花群珊瑚对两种微藻的摄食

王振, 杨阳楚翘, 陈芸, 王淑红*   

  1. 观赏水族福建省高校工程研究中心/集美大学水产学院, 福建厦门 361021
  • 收稿日期:2020-10-16 接受日期:2021-01-09 发布日期:2021-10-25
  • 通讯作者: *E-mail: shwang@jmu.edu.cn
  • 作者简介:王 振, 男, 1996年生, 硕士研究生。主要从事珊瑚异养营养研究。E-mail: 1262550229@qq.com
  • 基金资助:
    福建省自然科学基金项目(2018J01453)、厦门市海洋经济创新发展示范项目(16PZY002SF18)、福建省科技厅高校科研专项(JK2016022)和福建省教育厅产学研项目(JAT160250)资助

The feeding of Zoanthus sp. on two microalgae species.

WANG Zhen, YANG Yang-chu-qiao, CHEN Yun, WANG Shu-hong*   

  1. Ornamental Aquarium Engineering Research Centre in Universities of Fujian Province/Fisheries College of Jimei University, Xiamen 361021, Fujian, China
  • Received:2020-10-16 Accepted:2021-01-09 Published:2021-10-25
  • Contact: *E-mail: shwang@jmu.edu.cn
  • Supported by:
    Natural Science Foundation of Fujian Pro-vince, China (2018J01453), Demonstration of Marine Economy Innovative Development Project (16PZY002SF18), Research Fund for the Universities of Fujian Provincial Science and Technology Department (JK2016022), and Project on Industry-Education-Research of Fujian Provincial Education Department (JAT160250).

摘要: 以花群珊瑚为材料,采用碳清除率测定、PCR分子营养标记和组织学观察的方法,研究花群珊瑚是否摄食亚心形扁藻和球等鞭金藻。结果表明: 花群珊瑚对球等鞭金藻的碳清除率显著高于亚心形扁藻,分别为0.44和0.11 pg·mL-1·polyp-1·h-1。球等鞭金藻烯酰基载体蛋白还原酶基因片段作为分子标记在投喂组珊瑚组织中可扩增出目的片段。亚心形扁藻18S rRNA基因的引物在相应投喂组珊瑚组织中也可扩增出目的片段。组织学观察发现: 投喂组珊瑚水螅体内部隔膜较宽,隔膜与体壁的胃层都存在大量食物泡。亚心形扁藻投喂组在体壁胃层的食物泡中和口道沟处均发现未消化完全的亚心形扁藻。球等鞭金藻投喂组则在隔膜胃层和体壁胃层内的食物泡中发现所摄食金藻。因此,碳清除率、分子营养标记以及组织学观察均表明花群珊瑚对亚心形扁藻与球等鞭金藻存在摄食现象。

关键词: 花群珊瑚, 异养营养, 亚心形扁藻, 球等鞭金藻, 分子营养标记

Abstract: In this study, we investigated whether Zoanthus sp. feed on two common microalgae, Platymonas subcordiformis and Isochrysis galbana, using the methods of carbon clearance rate, DNA marker, and histological analyses. The results showed that carbon clearance rate of I. galbana by Zoanthus sp. was significantly higher than that of P. subcordiformis, which were 0.44 and 0.11 pg·mL-1·polyp-1·h-1, respectively. 162 bp of 18S rRNA gene from P. subcordiformis and 442 bp of enoyl-ACP reductase gene from I. galbana were used as molecular nutrition markers, both of them were successfully amplified from the Zoanthus sp. fed by both algae species. Results of the histological analyses demonstrated that pholyp from feeding group showed a widen mesentery. Lots of food vacuoles presented in tissues of mesentery and gastrodermis. Undigested cell body of P. subcordiformis and I. galbana could also be found in some food vacuoles around siphonoglyphe as well as the gastrodermis in body wall. Therefore, results from carbon clearance rate, histological and DNA marker results all indicated that Zoanthus sp. could feed on P. subcordiformis and I. galbana.

Key words: Zoanthus sp., heterotrophic nutrition, Platymonas subcordiformis, Isochrysis galbana, molecular nutrition indicator