太湖流域上游竹林河岸带土壤反硝化酶活性及其影响因素

doi:10.13287/j.1001-9332.202109.008

应用生态学报 ›› 2021, Vol. 32 ›› Issue (9): 3070-3078.doi: 10.13287/j.1001-9332.202109.008

太湖流域上游竹林河岸带土壤反硝化酶活性及其影响因素

周钟昱^1,2, 张海阔^1,3, 梁佳辉^1,3, 张宝刚^1,3, 蒋文婷^1,3, 田琳琳^1,2*, 李彦^1,2, 蔡延江^1,3

¹浙江农林大学省部共建亚热带森林培育国家重点实验室, 杭州 311300;
²浙江农林大学林业与生物技术学院, 杭州 311300;
³浙江农林大学环境与资源学院, 杭州 311300

收稿日期:2021-01-29 接受日期:2021-06-29 出版日期:2021-09-15 发布日期:2022-03-15
通讯作者: * E-mail: tianll@zafu.edu.cn
作者简介:周钟昱, 男, 1996年生, 硕士研究生。主要从事河岸带土壤反硝化脱氮研究。E-mail: zzy26chen@163.com
基金资助:
国家自然科学基金项目(41907268)、长江科学院开放基金项目(CKWV2019760/KY)和浙江农林大学校科研发展基金项目(2018FR005,2018FR006,2018FR061)资助

Soil denitrifying enzyme activity and its influencing factors in a bamboo forest riparian zone in the upper reaches of the Taihu Lake Basin, China

ZHOU Zhong-yu^1,2, ZHANG Hai-kuo^1,3, LIANG Jia-hui^1,3, ZHANG Bao-gang^1,3, JIANG Wen-ting^1,3, TIAN Lin-lin^1,2*, LI Yan^1,2, CAI Yan-jiang^1,3

¹State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China;
²College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China;
³College of Environment and Resources, Zhejiang A&F University, Hangzhou 311300, China

Received:2021-01-29 Accepted:2021-06-29 Online:2021-09-15 Published:2022-03-15
Contact: * E-mail: tianll@zafu.edu.cn
Supported by:
National Natural Science Foundation of China (41907268), the Open Fund of Changjiang River Scientific Research Institute (CKWV2019760/KY), and the Research and Development Fund of Zhejiang A&F University (2018FR005, 2018FR006, 2018FR061).

摘要/Abstract

摘要： 本研究以太湖流域上游竹林河岸带为对象,采用乙炔抑制法分析了夏季河岸带土壤反硝化酶活性(DEA)及其影响因素,以期为竹林河岸带在减少河流氮污染方面的生态功能评估提供数据支持。结果表明: 河岸带土壤DEA为6.32～23.22 μg N·kg^-1·h^-1,平均值为14.65 μg N·kg^-1·h^-1。河岸带土壤有机碳(SOC)、总氮、硝态氮含量、含水量和碳氮水解酶活性共同影响着DEA的垂直分布,使DEA随土壤深度(0～40 cm)的增加而递减;而DEA在水平方向上(同一土壤深度离水距离不同)的变化主要受SOC含量的影响。太湖流域上游竹林河岸带土壤在夏季可能会由于缺乏相对充足的可溶性有机碳而对DEA产生一定的限制。

关键词: 河岸带土壤, 反硝化酶活性, 有机碳, 硝态氮, 碳氮水解酶活性

Abstract: Soil denitrifying enzyme activity (DEA) was measured by acetylene inhibition technique, along with exploration of factors influencing DEA in a bamboo forest riparian zone in the upper reaches of the Taihu Lake Basin during summer. Our aim was to provide important insights into the assessment of ecological functions of bamboo forest riparian zone on reducing nitrogen pollution in rivers. The results showed that the riparian soil DEA ranged from 6.32 to 23.22 μg N·kg^-1·h^-1, with a mean value of 14.65 μg N·kg^-1·h^-1. The vertical distribution (0-40 cm soil profile) of DEA was affected by several factors, such as soil organic carbon (SOC), total nitrogen (TN), nitrate nitrogen (NO₃^--N), soil water content, and activity of carbon and nitrogen hydrolase, which resulted in decreased DEA with increasing soil depth. The horizontal changes in DEA (at the same soil depth but at different distances from river) was mainly governed by the variation in SOC concentration. In this area, the concentration of soil dissolved organic carbon was relatively low, which might inhibit the soil DEA during summer.

Key words: riparian zone soil, denitrifying enzyme activity, organic carbon, nitrate nitrogen, activity of carbon and nitrogen hydrolase

周钟昱, 张海阔, 梁佳辉, 张宝刚, 蒋文婷, 田琳琳, 李彦, 蔡延江. 太湖流域上游竹林河岸带土壤反硝化酶活性及其影响因素[J]. 应用生态学报, 2021, 32(9): 3070-3078.

ZHOU Zhong-yu, ZHANG Hai-kuo, LIANG Jia-hui, ZHANG Bao-gang, JIANG Wen-ting, TIAN Lin-lin, LI Yan, CAI Yan-jiang. Soil denitrifying enzyme activity and its influencing factors in a bamboo forest riparian zone in the upper reaches of the Taihu Lake Basin, China[J]. Chinese Journal of Applied Ecology, 2021, 32(9): 3070-3078.

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太湖流域上游竹林河岸带土壤反硝化酶活性及其影响因素

Soil denitrifying enzyme activity and its influencing factors in a bamboo forest riparian zone in the upper reaches of the Taihu Lake Basin, China

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