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应用生态学报 ›› 2021, Vol. 32 ›› Issue (12): 4447-4456.doi: 10.13287/j.1001-9332.202112.035

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基于转录组分析重金属镉对长茎葡萄蕨藻的影响

庞美霞1,2, 黄志立2, 刘小龙2, 唐勇军2, 李晓东2, 金刚2*   

  1. 1深圳职业技术学院博士后创新基地, 广东深圳 518055;
    2深圳职业技术学院应用化学与生物技术学院, 广东深圳 518055
  • 收稿日期:2021-02-05 修回日期:2021-09-18 出版日期:2021-12-15 发布日期:2022-06-15
  • 通讯作者: *E-mail: jingang@szpt.edu.cn
  • 作者简介:庞美霞, 女, 1989年生, 博士。主要从事分子生物学研究。E-mail: pang1mei2xia3@163.com
  • 基金资助:
    深圳职业技术学院博士后启动基金项目(6019330003K)和深圳市科技项目(JCYJ20170818140317993)资助

Effects of heavy metal cadmium on Caulerpa lentillifera based on transcriptome analysis

PANG Mei-xia1,2, HUANG Zhi-li2, LIU Xiao-long2, TANG Yong-jun2, LI Xiao-dong2, JIN Gang2*   

  1. 1Postdoctoral Innovation Practice Base, Shenzhen Polytechnic, Shenzhen 518055, Guangdong, China;
    2School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen 518055, Guangdong, China
  • Received:2021-02-05 Revised:2021-09-18 Online:2021-12-15 Published:2022-06-15
  • Contact: *E-mail: jingang@szpt.edu.cn
  • Supported by:
    Post-doctoral Foundation Project of Shenzhen Polytechnic (6019330003K) and the Science and Technology Project of Shenzhen City (JCYJ20170818140317993)

摘要: 随着人类社会工业化进程的加快,重金属镉(Cd)污染对海洋生物的毒害作用突显。为了探索Cd胁迫下长茎葡萄蕨藻生理调控的分子响应机制,本文采用RNA-Seq技术对镉胁迫(Hcd2+)藻体组织转录组进行研究。结果表明: 与对照相比,Hcd2+组共筛选出差异表达基因(DEGs)702个,其中,257个基因上调表达,445个基因下调表达。对获得的DEGs进行功能注释及富集分析,发现长茎葡萄蕨藻的多种生物学功能受到Cd2+胁迫的影响,最终表现为抑制生长。GO富集分析发现,长茎葡萄蕨藻体内氧自由基的产生与清除出现失衡,并产生了DNA损伤等氧化伤害。KEGG富集分析发现,长茎葡萄蕨藻中多条与光合作用相关途径受到抑制,表明Cd2+胁迫导致长茎葡萄蕨藻的光合反应紊乱。

关键词: 长茎葡萄蕨藻, 镉胁迫, 分子机制, 转录组

Abstract: With the acceleration of industrialization, the toxic effect of heavy metal cadmium (Cd) pollution has become prominent. In order to explore the molecular mechanism of the physiological regulation of Caulerpa lentillifera under Cd stress, we analyzed the transcriptome of Cd-stressed (Hcd2+) algae tissues using RNA-Seq. A total of 702 differentially expressed genes (DEGs) were screened between the control and Hcd2+ groups, out of which 257 genes were up-regulated and 445 genes were down-regulated in the Hcd2+ group. We conducted functional annotation and enrichment analysis of the obtained DEGs. The results showed that various biological functions of C. lentillifera were affected under Cd2+stress, which eventually showed growth inhibition. Results of GO enrichment analysis showed that the production and removal of reactive oxygen species (ROS) in C. lentillifera were out of balance and caused oxidative damage such as DNA damage. Results of KEGG enrichment analysis showed that many photosynthesis-related pathways were inhibited, indicating that Cd2+ stress led to disorder of photosynthetic reaction of C. lentillifera.

Key words: Caulerpa lentillifera, cadmium stress, molecular mechanism, transcriptome