电子受体和光照对土壤CH4排放相关微生物功能基因丰度的影响

doi:10.13287/j.1001-9332.202202.033

应用生态学报 ›› 2022, Vol. 33 ›› Issue (2): 517-526.doi: 10.13287/j.1001-9332.202202.033

电子受体和光照对土壤CH₄排放相关微生物功能基因丰度的影响

陈娟¹, 胡琳玉¹, 卢鹏伟¹, 江玉梅^1*, 张志斌¹, 曾庆桂¹, 简敏菲¹, 朱笃^1,2

¹江西师范大学生命科学学院, 江西省亚热带植物资源保护与利用重点实验室, 南昌 330022;
²江西科技师范大学生命科学学院, 江西省生物加工过程重点实验室, 南昌 330013

收稿日期:2021-04-08 修回日期:2021-11-17 出版日期:2022-02-15 发布日期:2022-08-15
通讯作者: *E-mail: leaf91626@163.com
作者简介:陈娟, 女, 1996年生, 硕士研究生。主要从事湿地生态及土壤微生物生态学研究。E-mail: 1835741012@qq.com
基金资助:
国家自然科学基金项目(31760161)、江西省自然科学基金项目(20202BABL203048)和江西省教育厅项目(GJJ160314)资助。

Effects of electron acceptor and light on the abundance of microbial function gene related to soil CH₄ emission

CHEN Juan¹, HU Lin-yu¹, LU Peng-wei¹, JIANG Yu-mei^1*, ZHANG Zhi-bin¹, ZENG Qing-gui¹, JIAN Min-fei¹, ZHU Du^1,2

¹Jiangxi Province Key Laboratory of Protection and Utilization of Subtropical Plant Resources, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China;
²Jiangxi Province Key Laboratory of Bioprocess Engineering, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, China

Received:2021-04-08 Revised:2021-11-17 Online:2022-02-15 Published:2022-08-15

摘要/Abstract

摘要： 为探究光照条件下添加不同电子受体对土壤甲烷排放的影响及微生物的响应,本研究在土壤中添加3种电子受体(Fe³⁺、NO₃^-、SO₄^2-)共设计8个处理,即黑暗+ Fe³⁺(DF)、黑暗+ NO₃^-(DN)、黑暗+ SO₄^2-(DS)、黑暗+蒸馏水(DCK)、光照+ Fe³⁺(LF)、光照+ NO₃^-(LN)、光照+SO₄^2-(LS)、光照+蒸馏水(LCK),通过20 d的严格厌氧培养,分析甲烷浓度的变化以及细菌、古菌、真菌及6种土壤功能微生物基因丰度的变化。结果表明: 除Fe³⁺处理组外,NO₃^-、SO₄^2-和对照(CK)组在光照条件下的甲烷排放显著低于黑暗条件下。土壤细菌、古菌、真菌丰度分别在DN、DCK、LF处理组中显著上调。产甲烷菌mcrA、硫酸盐还原菌Dsr、固碳菌CbbL基因丰度均在LF组中显著上调,而甲烷氧化菌pmoA、铁还原菌Geo、反硝化细菌nosZ基因丰度分别在LN、DCK、LCK组中显著上调。Pearson相关性及冗余分析表明,CH₄排放与CO₂浓度、pH、铵态氮、总氮含量呈显著正相关,与N₂O浓度、氧化还原电位、硝态氮、总碳含量呈显著负相关。黑暗条件下甲烷排放浓度与古菌、pmoA基因丰度呈正相关,与其他功能基因均呈负相关。光照条件下甲烷排放浓度与微生物及功能基因丰度均呈负相关。总体上,光照条件下的甲烷排放显著低于黑暗条件下(除Fe³⁺处理外),说明光照条件有助于甲烷减排,且甲烷排放的增减和环境中的电子受体种类及微生物的功能响应密切相关。

关键词: 电子受体, 湿地土壤, 甲烷排放, 光照, 功能基因丰度

Abstract: To explore the effects of different electron acceptors on soil methane emission and responses of soil microorganisms to different light conditions, a strict anaerobic 20-day incubation experiment was conducted with eight treatments: darkness + Fe³⁺(DF); darkness + NO₃^- (DN); darkness +SO₄^2-(DS); darkness + distilled water (DCK); light + Fe³⁺(LF); light + NO₃^- (LN); light +SO₄^2-(LS); light + distilled water (LCK). The changes of methane concentration in the anaerobic incubation flask and the variation of the abundance of bacteria, archaea, fungi and six soil functional genes were analyzed. Results showed that soil methane emission under NO₃^-, SO₄^2- addition and control (CK) was significantly lower under light conditions than dark, except the Fe³⁺ treatment. DN, DCK and LF treatments had the highest abundance of bacteria, fungi and archaea genes, respectively. The gene abundance of methanogenic mcrA, sulfate-reducing bacteria Dsr, and carbon-fixing CbbL were significantly up-regulated in the LF, while that of methanotrophs pmoA, iron-reducing bacteria Geo, and denitrifying bacteria nosZ were significantly up-regulated in the LN, DCK and LCK, respectively. Results of Pearson correlation and RDA analysis showed that CH₄ emission was significantly positively correlated with CO₂ concentration, pH, ammonium-nitrogen, and total N contents, and negatively correlated with N₂O concentration, Eh, nitrate, and total C contents. Under dark condition, methane emission was positively correlated with archaea and pmoA genes abundance, and negatively correlated with other genes abundance. Under light condition, methane emission was negatively correlated with the abundance of soil microbe and functional genes. In general, methane emission under light condition was significantly lower than that under dark condition (except for the Fe³⁺ treatment). These results showed that it was helpful to reduce methane emission under light condition, but the increase or decrease of methane emission was closely related to the type of electron acceptors and the functional responses of soil micro-organisms

Key words: electron acceptor, wetland soil, methane emission, illumination, abundance of functional genes

陈娟, 胡琳玉, 卢鹏伟, 江玉梅, 张志斌, 曾庆桂, 简敏菲, 朱笃. 电子受体和光照对土壤CH₄排放相关微生物功能基因丰度的影响[J]. 应用生态学报, 2022, 33(2): 517-526.

CHEN Juan, HU Lin-yu, LU Peng-wei, JIANG Yu-mei, ZHANG Zhi-bin, ZENG Qing-gui, JIAN Min-fei, ZHU Du. Effects of electron acceptor and light on the abundance of microbial function gene related to soil CH₄ emission[J]. Chinese Journal of Applied Ecology, 2022, 33(2): 517-526.

参考文献

[1] Kirschke S, Bousquet P, Ciais P, et al. Three decades of global methane sources and sinks. Nature Geoscience, 2013, 6: 813-823
[2] Wik M, Varner RK, Anthony KW, et al. Climate-sensitive northern lakes and ponds are critical components of methane release. Nature Geoscience, 2016, 9: 99-105
[3] 潘小翠, 管铭, 张崇邦. 互花米草入侵对滩涂湿地甲烷排放的影响. 应用生态学报, 2016, 27(4): 1145-1152
[4] 陈健, 申屠佳丽, 殷峻, 等. 甲烷厌氧氧化及其微生物特性研究进展. 环境污染与防治, 2018, 40(2): 222-229
[5] 隋心, 张荣涛, 钟海秀, 等. 森林生态系统中主要功能微生物的研究进展. 中国农学通报, 2014, 30(28): 1-5
[6] Sang YK, Veraart AJ, Meima Franke M, et al. Combined effects of carbon, nitrogen and phosphorus on CH₄ production and denitrification in wetland sediments. Geoderma, 2015, 259-260: 354-361
[7] Raghoebarsing AA, Pol A, van de Pas-Schoonen KT, et al. A microbial consortium couples anaerobic methane oxidation to denitrification. Nature, 2006, 440: 918-921
[8] Hinrichs KU, Hayes JM, Sylva SP, et al. Methane-consuming archaebacteria in marine sediments. Nature, 1999, 398: 802-805
[9] Orphan VJ, House CH, Hinrichs KU, et al. Methane-consuming archaea revealed by directly coupled isotopic and phylogenetic analysis. Science, 2001, 293: 484-487
[10] Beal EJ, House CH, Orphan VJ. Manganese and iron-dependent marine methane oxidation. Science, 2009, 325: 184-187
[11] 崔兴国, 范玉贞. 不同光照条件对白车轴草根际微生物的影响. 北方园艺, 2011(12): 150-151
[12] Jari TH, Hannu N, Turunen J, et al. Methane emissions from natural peatlands in the northern boreal zone in Finland. Fennoscandia Atmospheric Environment, 2003, 37: 147-151
[13] Long K, Flanagan LB, Cai TB. Diurnal and seasonal variation in methane emissions in a northern Canadian peatland measured by eddy covariance. Global Change Biology, 2010, 16: 2420-2435
[14] Chen WN, Zhang FY, Wang BX, et al. Diel and seasonal dynamics of ecosystem-sale methane flux and their determinants in an alpine meadow. Biogeosciences, 2019, 124: 1731-1745
[15] Kato Y, Yoshida H, Hattori T. Photoinduced non-oxidative coupling of methane over silica-alumina and alumina around room temperature. Chemical Communications, 1998, 7: 2389-2390
[16] 许振民, 卞振锋. 光催化甲烷转化研究进展. 物理化学学报, 2020, 36(3): 82-92
[17] 顾航, 肖凡书, 贺志理, 等. 湿地微生物介导的甲烷排放机制. 微生物学报, 2018, 58(4): 618-632
[18] Janet KJ, Kirsten SH. Soil microbiomes and climate change. Nature Reviews Microbiology, 2020, 18: 35-46
[19] 张前前, 胡启武, 冯哲, 等. 鄱阳湖沉水植物区不同深度土壤甲烷排放对温度水分变化的响应. 生态学报, 2020, 40(21): 7659-7667
[20] 田云飞, 安俊芳, 陶士敏. 重铬酸钾氧化-分光光度法测定土壤有机碳含量的研究. 现代化工, 2020, 40(4): 231-235
[21] Le Mer J, Roger P. Production, oxidation, emission and consumption of methane by soils: A review. European Journal of Soil Biology, 2001, 37: 25-50
[22] 巴哈尔古丽·别克吐尔逊, 叶尔江·拜克吐尔汉, 马哈亚·艾斯江. 邻菲罗啉比色法分析原始森林土壤中铁的含量. 光谱实验室, 2013, 30(5): 2314-2318
[23] 刘洋, 陈永娟, 王晓燕, 等. 水库与河流沉积物中好氧甲烷氧化菌群落差异性研究. 中国环境科学, 2018, 38(5): 1844-1854
[24] 王世全, 朴永哲, 崔玉波, 等. 基于pmoA基因的污泥干化芦苇床中甲烷氧化菌多样性分析. 安全与环境学报, 2015, 15(5): 235-238
[25] Somenahally AC, Hollister EB, Yan W, et al. Water management impacts on arsenic speciation and iron-reducing bacteria in contrasting rice-rhizosphere compartments. Environmental Science & Technology, 2011, 45: 8328-8335
[26] Dai X, Hu C, Zhang D, et al. Impact of a high ammonia-ammonium-pH system on methane-producing archaea and sulfate-reducing bacteria in mesophilic anaerobic digestion. Bioresource Technology, 2017, 245: 598-605
[27] Li S, Song L, Jin Y, et al. Linking N₂O emission from biochar-amended composting process to the abundance of denitrify (nirK and nosZ) bacteria community. AMB Express, 2016, 6: 37, doi: 10.1186/s13568-016-0208-x
[28] Chaudhary DR, Gautam RK, Yousuf B, et al. Nutrients, microbial community structure and functional gene abundance of rhizosphere and bulk soils of halophytes. Applied Soil Ecology, 2015, 91: 16-26
[29] 郭鹏豪, 刘秀丽, 崔颖鹏, 等. 真菌通用引物Its1和Its4在丝状真菌鉴定中的价值评价. 中国微生态学杂志, 2013, 25(8): 922-924
[30] 戴晓虎, 何进, 严寒, 等. 游离氨调控对污泥高含固厌氧消化反应器性能的影响. 环境科学, 2017, 38(2): 679-687
[31] Nardy K, Julia F, Yao P, et al. Global prevalence of methane oxidation by symbiotic bacteria in peat-moss ecosystems. Nature Geoscience, 2010, 3: 617-621
[32] Gabriele VM, Luciana C, Dayane J, et al. Methane emission suppression in flooded soil from Amazonia. Chemosphere, 2020, 250, doi: 10.1016/j.chemosphere.2020.126263
[33] Peters V, Conrad R. Sequential reduction processes and initiation of CH₄ production upon flooding of oxic upland soils. Soil Biology and Biochemistry, 1996, 28: 371-382
[34] 曾从盛, 王维奇, 仝川. 不同电子受体及盐分输入对河口湿地土壤甲烷产生潜力的影响. 地理研究, 2008, 27(6): 1321-1330
[35] Kluber DH, Conrad R. Inhibition effect of nitrate, nitrite, NO and N₂O on methanogenesis by Methanosarcina mazei. Microbiology Ecology, 1998, 25: 331-339
[36] 黄国宏, 李玉祥, 陈冠雄, 等. 环境因素对芦苇湿地CH₄排放的影响. 环境科学, 2001, 22(1): 1-5
[37] 蔡祖聪, 沈光裕, 颜晓元, 等. 土壤质地、温度和Eh对稻田甲烷排放的影响. 土壤学报, 1998, 35(2): 145-154
[38] 贾磊, 蒲旖旎, 杨诗俊, 等. 太湖藻型湖区CH₄、CO₂排放特征及其影响因素分析. 环境科学, 2018, 39(5): 2316-2329
[39] 王培, 叶文娟, 朱仁斌, 等. 南极苔原沼泽温室气体通量变化特征及其对气候变化的响应. 极地研究, 2020, 32(1): 13-24
[40] Li FF, Zhu RB, Bao T, et al. Sunlight stimulates methane uptake and nitrous oxide emission from the high Arctic tundra. Science of the Total Environment, 2016, 572: 1150-1160
[41] 邢亚薇, 李春越, 刘津, 等. 长期施肥对黄土旱塬农田土壤微生物丰度的影响. 应用生态学报, 2019, 30(4): 1351-1358
[42] Cao P, Zhang LM, Shen JP, et al. Distribution and diversity of archaeal communities in selected Chinese soils. FEMS Microbiology Ecology, 2012, 80: 146-158
[43] Xiao LL, Xie BH, Liu JC, et al. Stimulation of long-term ammonium nitrogen deposition on methanogenesis by Methanocellaceae in a coastal wetland. Science of the Total Environment, 2017, 595: 337-343
[44] 魏勃. 氨态氮对产甲烷菌的抑制及有机负荷提高引发的厌氧反应器微生物群落演. 博士论文. 西安: 西安建筑科技大学, 2016
[45] 闵航, 陈中云, 陈美慈. 水稻田土壤甲烷氧化活性及其环境影响因子的研究. 土壤学报, 2002, 39(5): 686-692
[46] 王静, 曲东. 硫酸盐作为共存电子受体对微生物Fe(Ⅲ)还原过程的影响. 西北农业学报, 2008, 17(6): 315-321
[47] Xiao LL, Liu FH, Liu JC, et al. Nano-Fe₃O₄ particles accelerating electro methanogenesis on an hour-long timescale in wetland soil. Environmental Science: Nano, 2018, 5: 436-445
[48] Xiao LL, Wei WC, Luo M, et al. A potential contribution of a Fe(Ⅲ)-rich red clay horizon to methane release: Biogenetic magnetite-mediated methanogenesis. Catena, 2019, 181: 104081, doi: 10.1016/j.catena.2019.104081
[49] 张晓波, 苏德荣, 赵艳. 不同光照条件下草地早熟禾根际微生物区系动态研究. 中国草地学报, 2007, 29(3): 23-27
[50] Yang J, Xiao W, Chi X, et al. Solar-driven efficient methane catalytic oxidation over epitaxial ZnO/La0.8Sr0.2CoO3 heterojunctions. Applied Catalysis B: Environmental, 2019, 265: 118469, doi: 10.1016/j.apcatb.2019.118469

电子受体和光照对土壤CH₄排放相关微生物功能基因丰度的影响

Effects of electron acceptor and light on the abundance of microbial function gene related to soil CH₄ emission

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	申诗怡, 王剑武, 周天焕, 马元丹, 王彬. 亚热带典型绿化灌木对夜间人工光照的生理响应 [J]. 应用生态学报, 2023, 34(9): 2321-2329.
[2]	张佳彭, 杨继松, 刘玥, 宁凯, 于君宝, 王志康, 王雪宏. 电子受体添加对黄河口湿地土壤厌氧碳矿化温度敏感性的影响 [J]. 应用生态学报, 2023, 34(11): 2985-2992.
[3]	施光耀, 桑玉强, 张劲松, 蔡露露, 张家兴, 孟平, 薛攀, 乔永胜. 不同光照强度下植物电信号变化特征及其与空气负离子的关系 [J]. 应用生态学报, 2022, 33(2): 439-447.
[4]	毛立, 孙志高, 陈冰冰, 胡星云, 武慧慧. 湿地土壤硫氧化-还原过程及其与其他元素的耦合作用研究进展 [J]. 应用生态学报, 2022, 33(2): 560-568.
[5]	马成祥, 周广胜, 宋兴阳, 吕晓敏. 增温、光照时间和氮添加对蒙古栎主要物候期的影响 [J]. 应用生态学报, 2022, 33(12): 3220-3228.
[6]	周光, 徐玮泽, 万静, 汪雁楠, 刘丽婷, 刘琪璟. 长白山阔叶红松林不同演替阶段林下红松幼苗能量与养分季节动态 [J]. 应用生态学报, 2021, 32(5): 1663-1672.
[7]	邓思宇, 陈袁波, 余珂, 于志国. 不同类型泥炭沼泽湿地无机离子、溶解有机质的变化特征及生态学意义 [J]. 应用生态学报, 2021, 32(2): 571-580.
[8]	兰建英, 蒋海明, 李侠. 微生物种间直接电子传递研究进展 [J]. 应用生态学报, 2021, 32(1): 358-368.
[9]	周爽男, 陈奇成, 江茂旺, 蒋霞敏, 彭瑞冰, 韩庆喜, 黄晨, 赵晨曦, 李建平. 光照强度对虎斑乌贼生长、存活、代谢及相关酶活性的影响 [J]. 应用生态学报, 2019, 30(6): 2072-2078.
[10]	王筱彤，孔范龙，郗敏*，李悦，隋晓敏. 胶州湾典型河口湿地土壤溶解性无机碳分布特征 [J]. 应用生态学报, 2018, 29(6): 2043-2050.
[11]	周爽男,吕腾腾,陈奇成,彭瑞冰,韩庆喜,蒋霞敏. 光照强度与光周期对虎斑乌贼胚胎发育的影响 [J]. 应用生态学报, 2018, 29(6): 2059-2067.
[12]	姜恒, 邹定辉, 娄文勇. 无机碳供应与光照条件对坛紫菜光合功能的影响 [J]. 应用生态学报, 2018, 29(2): 515-521.
[13]	徐祥增, 张金燕, 张广辉, 龙光强, 杨生超, 陈中坚, 魏富刚, 陈军文. 光强对三七光合能力及能量分配的影响 [J]. 应用生态学报, 2018, 29(1): 193-204.
[14]	付忠,谢世清,徐文果,岩所,陈军文. 不同光照强度下谢君魔芋的光合作用及能量分配特征 [J]. 应用生态学报, 2016, 27(4): 1177-1188.
[15]	余意,杨其长,刘文科**. LED短期连续光照与氮营养对水培生菜品质的影响 [J]. 应用生态学报, 2015, 26(11): 3361-3366.