生石灰对林下酸化土壤的调控作用及三七生长的影响

doi:10.13287/j.1001-9332.202204.018

摘要/Abstract

摘要： 土壤酸化是导致林下三七生长不良和根腐病发生的重要因素。本试验利用不同量(0、500、1000、1500和2000 kg·hm^-2)的生石灰改良林下酸化土壤,评价生石灰对土壤理化性质、酚类物质、根际微生物和三七生长的影响。结果表明: 适量的生石灰(500～1000 kg·hm^-2)可显著提高土壤pH值,降低酚类物质(羟基苯甲酸、香草醛、丁香酸、阿魏酸和香草酸)含量,促进三七的生长,并降低根腐病的发生。适量的生石灰(500～1000 kg·hm^-2)能显著降低土壤的真菌/细菌,提高细菌多样性,提高门水平上子囊菌门、变形菌门和属水平上马赛菌属和鞘脂单胞菌属的相对丰度。但是过量的生石灰(1500～2000 kg·hm^-2)会降低土壤碱解氮和有机质含量,抑制三七的生长。因此,500～1000 kg·hm^-2生石灰可显著改善林下土壤的化学性质和微生物群落,从而促进三七生长,降低根腐病的发生。

关键词: 生石灰, 土壤酸化, 酚类物质, 微生物群落

Abstract: Soil acidification is an important factor leading to poor growth and root rot disease of Panax notoginseng in the understorey of forests. In this study, different amounts of quicklime (0, 500, 1000, 1500 and 2000 kg·hm^-2) were amended into acid soil under forest. We evaluated the effect of quikelime addition on soil chemical properties, phenols, rhizosphere microorganisms and growth of P. notoginseng. The results showed that an appro-priate amount of quicklime addition (500-1000 kg·hm^-2) could significantly increase soil pH, decrease the content of phenols (p-hydroxybenzoic acid, vanillin, syringic acid, ferulic acid and vanillic acid), promote P. notoginseng growth, and reduce the incidence of root rot disease. An appropriate amount of quicklime (500-1000 kg·hm^-2) could significantly reduce the fungi:bacteria ratio, increase bacteria diversity, and increase the relative abundance of Ascomycota and Proteobacteria as well as Massilia and Sphingomonas. However, excessive quicklime addition (1500-2000 kg·hm^-2) could reduce the content of available nitrogen and organic matter, and inhibit P. notoginseng growth. Therefore, 500-1000 kg·hm^-2 of quicklime amendment could improve the chemical properties and microbial community of acid soil under forest, thereby promoting P. notoginseng growth, and reducing the incidence of root rot disease.

Key words: quicklime, soil acidification, phenols, microbial community

张义杰, 徐杰, 陆仁窗, 叶辰, 黄惠川, 杨敏, 何霞红, 朱书生. 生石灰对林下酸化土壤的调控作用及三七生长的影响[J]. 应用生态学报, 2022, 33(4): 972-980.

ZHANG Yi-jie, XU Jie, LU Ren-chuang, YE Chen, HUANG Hui-chuan, YANG Min, HE Xia-hong, ZHU Shu-sheng. Modification of quicklime on acid soil under forest and their effect on the growth of Panax notoginseng[J]. Chinese Journal of Applied Ecology, 2022, 33(4): 972-980.

参考文献

[1] Donald PF. Society for conservation biology biodiversity impacts of some agricultural commodity production systems. Conservation Biology, 2004, 18: 17-37
[2] Wu HM, Xia JS, Qin XJ, et al. Underlying mechanism of wild Radix pseudostellariae in tolerance to disease under the natural forest cover. Frontiers in Microbiology, 2020, 11: 1142-1151
[3] 荆淑芹, 姜海平, 刘凤云, 等. 生晒参红参林下参中7种人参皂苷含量的比较. 中华中医药学刊, 2009, 27(1): 207-209
[4] Ye C, Fang HY, Liu HJ, et al. Current status of soil sickness research on Panax notoginseng in Yunnan, China. Allelopathy Journal, 2019, 47: 1-14
[5] 邓琳梅, 杨蕾, 张俊星, 等. 云南省不同森林土壤中三七促生拮抗细菌的分离筛选. 南方农业学报, 2020, 51(1): 115-122
[6] Tang C. Factors affecting soil acidification under legumes. I. Effect of potassium supply. Plant and Soil, 1998, 199: 275-282
[7] Xiang J, Ryan HV, Peng S, et al. Improvement in nitrogen availability, nitrogen uptake and growth of aerobic rice following soil acidification. Soil Science and Plant Nutrition, 2010, 55: 705-714
[8] Wei W, Yang M, Liu Y, et al. Fertilizer N application rate impacts plant-soil feedback in a sanqi production system. Science of the Total Environment, 2018, 633: 796-807
[9] Yang M, Chuan Y, Guo C, et al. Panax notoginseng root cell death caused by the autotoxic ginsenoside Rg1 is due to over-accumulation of ROS, as revealed by trans-criptomic and cellular approaches. Frontiers in Plant Science, 2018, 28: 264
[10] Liu HJ, Yang M, Zhu SS. Strategies to solve the problem of soil sickness of Panax notoginseng (family: Araliaceae). Allelopathy Journal, 2019, 47: 37-56
[11] Dai Z, Zhang X, Tang C, et al. Potential role of biochars in decreasing soil acidification: A critical review. Science of the Total Environment, 2017, 581: 601-611
[12] Saing Z, Ibrahim MH, Irianto. Experimental investigation on strength improvement of lateritic Halmahera soil using quicklime stabilization. The 3rd International Conference on Civil and Environmental Engineering (ICCEE 2019), Bali, Indonesia, 2019: 419: 012013
[13] Xun W, Zhao J, Xue C, et al. Significant alteration of soil bacterial communities and organic carbon decomposition by different long-term fertilization management conditions of extremely low-productivity arable soil in South China. Environmental Microbiology, 2016, 18: 1907-1917
[14] 乔鈜元, 盛月凡, 王海燕, 等. 生石灰与过磷酸钙混施对连作土壤的改良效果及平邑甜茶幼苗生长的影响. 中国果树, 2020(3): 16-22
[15] Bai Y, Wang G, Cheng Y, et al. Soil acidification in continuously cropped tobacco alters bacterial community structure and diversity via the accumulation of phenolic acids. Scientific Reports, 2019, 9: 12499
[16] Abdalmoula MM, Makineci E, Özturna AG, et al. Soil organic carbon accumulation and several physicochemical soil properties under stone pine and maritime pine plantations in coastal dune, Durusu-Istanbul. Geoderma, 2019, 191: 312
[17] De Falco G, Magni P, TerSvuori LMH, et al. Sediment grain size and organic carbon distribution in the Cabras lagoon (Sardinia, Western Mediterranean). Chemistry and Ecology, 2004, 20: 367-377
[18] Tkaczyk P, Bednarek W, Dresler S, et al. The effect of some soil physicochemical properties and nitrogen ferti-lisation on winter wheat yield. Acta Agrophysica, 2018, 25: 107-116
[19] Chakraborty S, Weindorf DC, Zhu Y, et al. Spectral reflectance variability from soil physicochemical properties in oil contaminated soils. Geoderma, 2012, 177: 80-89
[20] Supapong P, Rungruang L, Phairat R, et al. Soil physicochemical properties related to the presence of Burkholderia pseudomallei. Royal Society of Tropical Medicine and Hygiene, 2008, 102: 5-9
[21] Souto C, Pellissier F, Chiapusio G. Allelopathic effects of humus phenolics on growth and respiration of mycorrhizal fungi. Journal of Chemical Ecology, 2000, 26: 2015-2023
[22] Xiao H, Wang B, Lu S, et al. Soil acidification reduces the effects of short-term nutrient enrichment on plant and soil biota and their interactions in grasslands. Global Change Biology, 2020, 26: 4626-4637
[23] 淡俊豪, 齐绍武, 黎娟, 等. 生石灰对酸性土壤pH值及微生物群落功能多样性的影响. 西南农业学报, 2017, 30(12): 2739-2745
[24] 周武先, 何银生, 朱盈徽, 等. 生石灰和钙镁磷肥对酸化川党参土壤的改良效果. 应用生态学报, 2019, 30(9): 3224-3232
[25] Schrijver AD, Frenne PD, Staelens J, et al. Tree species traits cause divergence in soil acidification during four decades of postagricultural forest development. Global Change Biology, 2012, 18: 1127-1140
[26] 王志康, 徐子恒, 陈紫云, 等. 有机肥和解磷固氮菌配施对缺碳黄棕壤养分特性的协同效应. 应用生态学报, 2020, 31(10): 3413-3423
[27] 杨敏, 和明东, 段杰, 等. 生物炭对连作烤烟根际土壤酚酸类物质及微生物群落结构的影响. 福建农业学报, 2020, 35(1): 107-114
[28] 涂玉婷, 黄继川, 彭智平, 等. 生物炭对酚酸胁迫下番茄生长和土壤微生态的影响. 广东农业科学, 2021, 48(1): 94-103
[29] Zhou X, Wu F. Vanillic acid changed cucumber (Cucumis sativus L.) seedling rhizosphere total bacterial, Pseudomonas and Bacillus spp. communities. Scientific Reports, 2018, 8: 4929
[30] Heijden M, Wagg C. Soil microbial diversity and agro-ecosystem functioning. Plant and Soil, 2013, 363: 1-5
[31] 朱永官, 彭静静, 韦中, 等. 土壤微生物组与土壤健康. 中国科学: 生命科学, 2021, 51(1): 1-11
[32] Saleem M, Hu J, Jousset A. More than the sum of its parts: Microbiome biodiversity as a driver of plant growth and soil health. Annual Review of Ecology, Evolution and Systematics, 2019, 50: 145-168
[33] Beimforde C, Feldberg K, Nylinder S, et al. Estimating the phanerozoic history of the Ascomycota lineages: Combining fossil and molecular data. Molecular Phylogenetics and Evolution, 2014, 78: 386-398
[34] 萨如拉, 杨恒山, 邰继承, 等. 秸秆还田条件下腐熟剂对不同质地土壤真菌多样性的影响. 中国生态农业学报, 2020, 28(7): 121-131
[35] Ward NL, Challacombe JF, Janssen PH, et al. Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils. Applied and Environmental Microbiology, 2009, 75: 2046-2056
[36] Wei M, Xu C, Xu X, et al. Characteristics of atmospheric bacterial and fungal communities in PM2.5 following biomass burning disturbance in a rural area of North China Plain. Science of the Total Environment, 2018, 651: 2727-2739
[37] Coppotelli BM, Ibarrolaza A, Panno M, et al. Effects of the inoculant strain Sphingomonas paucimobilis 20006FA on soil bacterial community and biodegradation in phenanthrene-contaminated soil. Microbial Ecology, 2008, 55: 173-183