对二甲苯对皱纹盘鲍肝胰腺细胞DNA的损伤

doi:10.13287/j.1001-9332.202206.015

应用生态学报 ›› 2022, Vol. 33 ›› Issue (6): 1679-1685.doi: 10.13287/j.1001-9332.202206.015

对二甲苯对皱纹盘鲍肝胰腺细胞DNA的损伤

许翠娅^*

福建省水产研究所, 福建厦门 361013

收稿日期:2021-06-09 接受日期:2022-02-16 发布日期:2022-12-15
通讯作者: *E-mail: 1050150353@qq.com
作者简介:许翠娅, 女, 1973年生, 高级工程师. 主要从事渔业环境生态和水产品质量安全研究. E-mail: 1050150353@qq.com
基金资助:
福建省属公益类科研院所基本科研专项(2015R1003-1,2020R1013006)、福建省海洋与渔业结构调整专项(2019HYJG07)和福建省海洋与渔业保护及发展专项(AQQQ005201902)资助。

DNA damage in hepatopancreas cells of Haliotis discus hannai induced by P-xylene

XU Cui-ya

Fisheries Research Institute of Fujian, Xiamen 361013, Fujian, China

Received:2021-06-09 Accepted:2022-02-16 Published:2022-12-15

摘要/Abstract

摘要： 为研究对二甲苯对皱纹盘鲍肝胰腺的毒性作用,设置4个浓度(0.5、1.0、1.5和2.0 mg·L^-1)和对照组,开展为期21 d的对二甲苯对皱纹盘鲍的亚慢性毒性试验,采用彗星试验技术进行皱纹盘鲍肝胰腺细胞DNA损伤分析,采用CASP分析软件对拖尾率、彗星尾长、彗尾DNA相对含量、Olive矩等损伤指标进行统计。结果表明: 与对照组相比,各染毒组皱纹盘鲍肝胰腺细胞DNA均受到损伤,且损伤程度存在显著性差异。随着染毒浓度的增加,肝胰腺细胞DNA受损程度加重,高浓度甚至可以引发细胞凋亡,呈现一定的剂量-损伤效应。中浓度对二甲苯短时间暴露即可对皱纹盘鲍肝胰腺细胞造成DNA损伤,随着暴露时间延长,细胞DNA受损程度加重,呈现一定的时间-损伤效应。但长时间暴露细胞DNA各损伤指标有所减小,这可能与细胞自身的DNA修复机制和生物体解毒系统的代谢机制有关。研究表明,对二甲苯可对皱纹盘鲍肝胰腺细胞产生氧化损伤,导致DNA断裂,高浓度的对二甲苯长时间暴露可导致其细胞凋亡。

关键词: 对二甲苯, 皱纹盘鲍, DNA损伤, 彗星试验

Abstract: In order to examine the toxic effects of p-xylene on the hepatopancreas of Haliotis discus hannai, a subchronic toxicity test was carried out for 21 days by setting 4 concentrations (0.5, 1.0, 1.5, and 2.0 mg·L^-1) and the control group. DNA damage in hepatopancreas cells of H. discus hannai was analyzed by comet assay. The CASP analysis software was used to analyze the damage indices, including tail rate, tail length, percentage of tail DNA, and olive moment. The results showed that DNA damage in hepatopancreas cells occured in all exposed groups, with different degrees of damage. The DNA damage of hepatopancreas cells was aggravated with the increase of exposure concentration. High concentration of p-xylene could induce apoptosis, showing a certain dose-damage effect. Short-term exposure to medium concentration of p-xylene could cause DNA damage in hepatopancreas cells of H. discus hannai, and the damage degree increased with the extension of exposure time, showing a certain time-damage effect. However, the damage indices of DNA decreased after prolonged exposure, which might be related to the DNA repair mechanism of the cell and the metabolic mechanisms of the detoxification system. In conclusion, p-xylene could induce oxidative damage in hepatopancreas cells of H. discus hannai, resulting in DNA fragmentation. Prolonged exposure to high concentration of p-xylene could lead to apoptosis.

Key words: p-xylene, Haliotis discus hannai, DNA damage, comet assay

许翠娅. 对二甲苯对皱纹盘鲍肝胰腺细胞DNA的损伤[J]. 应用生态学报, 2022, 33(6): 1679-1685.

XU Cui-ya. DNA damage in hepatopancreas cells of Haliotis discus hannai induced by P-xylene[J]. Chinese Journal of Applied Ecology, 2022, 33(6): 1679-1685.

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对二甲苯对皱纹盘鲍肝胰腺细胞DNA的损伤

DNA damage in hepatopancreas cells of Haliotis discus hannai induced by P-xylene

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