氮添加对毛竹林土壤酸性磷酸单酯酶动力学参数的影响

doi:10.13287/j.1001-9332.202208.018

应用生态学报 ›› 2022, Vol. 33 ›› Issue (8): 2178-2186.doi: 10.13287/j.1001-9332.202208.018

氮添加对毛竹林土壤酸性磷酸单酯酶动力学参数的影响

曾泉鑫^1,2, 元晓春^1,2,3, 周嘉聪^1,2, 吴君梅^1,2, 李文周⁴, 林惠瑛^1,2, 张晓晴^1,2, 陈岳民^1,2*

¹福建师范大学地理科学学院, 福州 350007;
²福建师范大学湿润亚热带山地生态国家重点实验室培育基地, 福州 350007;
³武夷学院旅游学院, 福建武夷山 354300;
⁴福建戴云山国家级自然保护区管理局, 福建泉州 362500

收稿日期:2021-10-02 接受日期:2022-05-26 出版日期:2022-08-15 发布日期:2023-02-15
通讯作者: * E-mail: ymchen@fjnu.edu.cn
作者简介:曾泉鑫, 男, 1995年生, 博士研究生。主要从事全球变化背景下森林生态系统磷循环和土壤微生物研究。E-mail: 645823769@qq.com
基金资助:
福建省自然科学基金项目(2019J05163,2020J01142,2020J01397)、安徽省自然科学基金项目(2108085QC105)和南平市自然科学基金项目(2019J03)资助。

Effects of nitrogen addition on the kinetic parameters of soil acid phosphomonoesterase in a Moso bamboo forest

ZENG Quan-xin^1,2, YUAN Xiao-chun^1,2,3, ZHOU Jia-cong^1,2, WU Jun-mei^1,2, LI Wen-zhou⁴, LIN Hui-ying^1,2, ZHANG Xiao-qing^1,2, CHEN Yueh-min^1,2*

¹School of Geographical Science, Fujian Normal University, Fuzhou 350007, China;
²Cultivation Base of State Key Laboratory of Humid Subtropical Mountain Ecology, Fujian Normal University, Fuzhou 350007, China;
³College of Tourism, Wuyi University, Wuyishan 354300, Fujian, China;
⁴Daiyun Mountain National Nature Reserve Administration Bureau, Quanzhou 362500, Fujian, China

Received:2021-10-02 Accepted:2022-05-26 Online:2022-08-15 Published:2023-02-15

摘要/Abstract

摘要： 土壤磷酸酶在有机磷矿化和磷循环过程中发挥着重要作用,然而,土壤磷酸酶响应氮(N)沉降的动力学机制仍不清楚。本研究在亚热带毛竹林中设置对照(0)、20(低氮)、40(中氮)和80 g N·hm^-2·a^-1(高氮)4种不同氮添加处理,在氮添加满3年、5年和7年时采集0～15 cm土层土壤样本,测定了土壤化学性质、微生物生物量,并分析了酸性磷酸单酯酶(ACP)的最大反应速率(V_m)、半饱和常数(K_m)和催化效率(K_a)。结果表明: 氮添加显著降低了土壤可溶性有机碳、有效磷和有机磷含量,显著增加了土壤铵态氮、硝态氮含量和V_m,且V_m与有效磷、有机磷和可溶性有机碳含量存在显著相关关系;总体上,氮添加显著提高了K_a;除了在氮添加满5年时高氮处理下K_m显著高于对照外,氮添加对K_m无显著影响,且K_m与有效磷和有机磷含量有显著负相关关系。中、高氮处理对ACP动力学参数的影响大于低氮处理。方差分解分析表明,土壤化学性质的变化而非微生物学性质的变化主导了V_m(47%)和K_m(33%)的变化。总之,氮添加显著影响了毛竹林土壤的基质有效性,通过调控ACP动力学参数(尤其是V_m)进而影响了土壤磷循环。本研究有助于了解氮素富集下土壤微生物调节土壤磷循环的潜在机制,并为全球变化下土壤磷循环模型优化提供重要参数。

关键词: 氮添加, 酸性磷酸单酯酶动力学参数, 基质有效性, 有机磷, 土壤磷循环

Abstract: Soil phosphatases are important in the mineralization of organophosphates and in the phosphorus (P) cycle. The kinetic mechanisms of phosphatases in response to nitrogen (N) deposition remain unclear. We carried out a field experiment with four different concentrations of N: 0 g N·hm^-2·a^-1(control), 20 g N·hm^-2·a^-1(low N), 40 g N·hm^-2·a^-1(medium N), and 80 g N·hm^-2·a^-1(high N) in a subtropical Moso bamboo forest. Soil samples were then collected from 0 to 15 cm depth, after 3, 5 and 7 years of N addition. We analyzed soil chemical properties and microbial biomass. Acid phosphatase (ACP) was investigated on the basis of maximum reaction velocity (V_m), Michaelis constant (K_m), and catalytic efficiency (K_a). Results showed that N addition significantly decreased soil dissolved organic carbon (DOC), available phosphorus, and organophosphate content, but significantly increased soil ammonium, nitrate-N content, and V_m. There was a significant relationship between V_m and the concentrations of available phosphorus, organophosphate, and soil DOC. In general, N addition substantially increased K_a, but did not affect K_m. The K_m value in the high N treatment group was higher than that in the control group after five years of N addition. K_m was significantly negatively associated with both available phosphorus and organophosphate. Medium and high N treatments had stronger effects on the kinetic parameters of ACP than low N treatment. Results of variation partition analysis showed that changes in soil chemical properties, rather than microbial biomass, dominated changes in V_m(47%) and K_m(33%). In summary, N addition significantly affected substrate availability in Moso bamboo forest soil and modulated soil P cycle by regulating ACP kinetic parameters (especially V_m). The study would improve the understanding of the mechanisms underlying soil microorganisms-regulated soil P cycle under N enrichment. These mechanisms would identify the important parameters for improving soil P cycling models under global change scenarios.

Key words: nitrogen addition, acid phosphatase kinetic parameter, substrate available, organic phosphorus, soil phosphorus cycle

曾泉鑫, 元晓春, 周嘉聪, 吴君梅, 李文周, 林惠瑛, 张晓晴, 陈岳民. 氮添加对毛竹林土壤酸性磷酸单酯酶动力学参数的影响[J]. 应用生态学报, 2022, 33(8): 2178-2186.

ZENG Quan-xin, YUAN Xiao-chun, ZHOU Jia-cong, WU Jun-mei, LI Wen-zhou, LIN Hui-ying, ZHANG Xiao-qing, CHEN Yueh-min. Effects of nitrogen addition on the kinetic parameters of soil acid phosphomonoesterase in a Moso bamboo forest[J]. Chinese Journal of Applied Ecology, 2022, 33(8): 2178-2186.

参考文献

[1] Fan Y, Lu S, He M, et al. Long-term throughfall exclusion decreases soil organic phosphorus associated with reduced plant roots and soil microbial biomass in a subtropical forest. Geoderma, 2021, 404: 115309
[2] Liu Y, Tan X, Wang Y, et al. Responses of litter, organic and mineral soil enzyme kinetics to 6 years of canopy and understory nitrogen additions in a temperate forest. Science of the Total Environment, 2020, 712: 136383
[3] Tan X, Nie Y, Ma X, et al. Soil chemical properties rather than the abundance of active and potentially active microorganisms control soil enzyme kinetics. Science of the Total Environment, 2021, 770: 144500
[4] Razavi BS, Blagodatskaya E, Kuzyakov Y. Temperature selects for static soil enzyme systems to maintain high catalytic efficiency. Soil Biology and Biochemistry, 2016, 97: 15-22
[5] Zhang X, Yang Y, Zhang C, et al. Contrasting responses of phosphatase kinetic parameters to nitrogen and phosphorus additions in forest soils. Functional Ecology, 2018, 32: 106-116
[6] Stone MM, Plante AF. Changes in phosphatase kinetics with soil depth across a variable tropical landscape. Soil Biology and Biochemistry, 2014, 71: 61-67
[7] Liu W, Tian R, Peng Z, et al. Nonlinear responses of the V_max and K_m of hydrolytic and polyphenol oxidative enzymes to nitrogen enrichment. Soil Biology and Biochemistry, 2020, 141: 107656
[8] 董清馨, 张心昱, 王辉民, 等. 氮添加对杉木林土壤有机碳矿化速率及酶动力学参数温度敏感性的影响. 生态学报, 2018, 38(18): 6502-6510
[9] Xu Z, Yu G, Zhang X, et al. Soil enzyme activity and stoichiometry in forest ecosystems along the North-South Transect in eastern China (NSTEC). Soil Biology and Biochemistry, 2017, 104: 152-163
[10] Zhu J, He N, Wang Q, et al. The composition, spatial patterns, and influencing factors of atmospheric wet nitrogen deposition in Chinese terrestrial ecosystems. Science of the Total Environment, 2015, 511: 777-785
[11] Hui D, Mayes MA, Wang G. Kinetic parameters of phosphatase: A quantitative synthesis. Soil Biology and Biochemistry, 2013, 65: 105-113
[12] Tang J, Riley WJ. Competitor and substrate sizes and diffusion together define enzymatic depolymerization and microbial substrate uptake rates. Soil Biology and Biochemistry, 2019, 139: 107624
[13] Ramin KI, Allison SD. Bacterial tradeoffs in growth rate and extracellular enzymes. Frontiers in Microbiology, 2019, 10: 2956
[14] Wallenstein M, Allison SD, Ernakovich J, et al. Controls on the temperature sensitivity of soil enzymes: A key driver of in situ enzyme activity rates// Shukla G, Varma A, eds. Soil Enzymology. New York: Springer, 2011: 245-258
[15] Carrara JE, Walter CA, Hawkins JS, et al. Interactions among plants, bacteria, and fungi reduce extracellular enzyme activities under long-term N fertilization. Global Change Biology, 2018, 24: 2721-2734
[16] 曾泉鑫, 曾晓敏, 林开淼. 亚热带毛竹林土壤磷组分和微生物对施氮的响应. 应用生态学报, 2020, 31(3): 753-760
[17] Tischer A, Blagodatskaya E, Hamer U. Microbial community structure and resource availability drive the catalytic efficiency of soil enzymes under land-use change conditions. Soil Biology and Biochemistry, 2015, 89: 226-237
[18] Li Q, Song X, Gu H, et al. Nitrogen deposition and management practices increase soil microbial biomass carbon but decrease diversity in Moso bamboo plantations. Scientific Reports, 2016, 6: 28235
[19] Song X, Zhou G, Jiang H, et al. Carbon sequestration by Chinese bamboo forests and their ecological benefits: Assessment of potential, problems, and future challenges. Environmental Reviews, 2011, 19: 418-428
[20] Li Y, Feng P. Bamboo resources in China based on the ninth National Forest Inventory data. World Bamboo Rattan, 2019, 17: 45-48
[21] 曾泉鑫, 张秋芳, 林开淼, 等. 酶化学计量揭示5年氮添加加剧毛竹林土壤微生物碳磷限制. 应用生态学报, 2021, 32(2): 521-528
[22] Cui J, Yuan X, Zhang Q, et al. Nutrient availability is a dominant predictor of soil bacterial and fungal community composition after nitrogen addition in subtropical acidic forests. PLoS One, 2021, 16(2): e0246263
[23] 程蕾, 周嘉聪, 林开淼, 等. 氮添加对亚热带毛竹林土壤微生物群落结构的影响. 生态学杂志, 2020, 39(6): 1929-1937
[24] Mo J, Zhang W, Zhu W, et al. Nitrogen addition reduces soil respiration in a mature tropical forest in sou-thern China. Global Change Biology, 2008, 14: 403-412
[25] Zhang J, Tian P, Tang J, et al. The characteristics of soil N transformations regulate the composition of hydrologic N export from terrestrial ecosystem. Journal of Geophysical Research: Biogeosciences, 2016, 121: 1409-1419
[26] Carter MR. Soil Sampling and Methods of Analysis. Boca Raton, FL, USA: The Chemical Rubber Company Press, 1993
[27] Vance ED, Brookes PC, Jenkinson DS. An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry, 1987, 19: 703-707
[28] Saiya-Cork KR, Sinsabaugh RL, Zak DR. The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biology and Biochemistry, 2002, 34: 1309-1315
[29] German DP, Weintraub MN, Grandy AS, et al. Optimization of hydrolytic and oxidative enzyme methods for ecosystem studies. Soil Biology and Biochemistry, 2011, 43: 1387-1397
[30] Belsley DA, Kuh E, Welsch RE. Regression Diagnostics: Identifying Influential Data and Sources of Collinearity. New York: Wiley-Interscience, 2005
[31] 陈倩妹, 王泽西, 刘洋. 川西亚高山针叶林土壤酶及其化学计量比对模拟氮沉降的响应. 应用与环境生物学报, 2019, 25(4): 791-800
[32] Houlton BZ, Wang YP, Vitousek PM, et al. A unifying framework for dinitrogen fixation in the terrestrial biosphere. Nature, 2008, 454: 327-330
[33] Wang C, Lu X, Mori T, et al. Responses of soil microbial community to continuous experimental nitrogen additions for 13 years in a nitrogen-rich tropical forest. Soil Biology and Biochemistry, 2018, 121: 103-112
[34] Mori T, Lu X, Aoyagi R, et al. Reconsidering the phosphorus limitation of soil microbial activity in tropical forests. Functional Ecology, 2018, 32: 1145-1154
[35] 程蕾, 林开淼, 周嘉聪, 等. 氮沉降对毛竹林土壤可溶性有机质数量与光谱学特征的影响. 应用生态学报, 2019, 30(5): 1754-1762
[36] Zak DR, Freedman ZB, Upchurch RA, et al. Anthropogenic N deposition increases soil organic matter accumulation without altering its biochemical composition. Global Change Biology, 2017, 23: 933-944
[37] Burns RG, DeForest JL, Marxsen J, et al. Soil enzymes in a changing environment: Current knowledge and future directions. Soil Biology and Biochemistry, 2013, 58: 216-234
[38] Zhang A, Chen Z, Zhang G, et al. Soil phosphorus composition determined by ³¹P NMR spectroscopy and relative phosphatase activities influenced by land use. European Journal of Soil Biology, 2012, 52: 73-77
[39] Li Q, Lv J, Peng C, et al. Nitrogen-addition accelerates phosphorus cycling and changes phosphorus use strategy in a subtropical Moso bamboo forest. Environmental Research Letters, 2021, 16: 024023
[40] Chen J, van Groenigen KJ, Hungate BA, et al. Long-term nitrogen loading alleviates phosphorus limitation in terrestrial ecosystems. Global Change Biology, 2020, 26: 5077-5086
[41] Shaw AN, Cleveland CC. The effects of temperature on soil phosphorus availability and phosphatase enzyme activities: A cross-ecosystem study from the tropics to the Arctic. Biogeochemistry, 2020, 151: 113-125
[42] 李银, 曾曙才, 黄文娟. 模拟氮沉降对鼎湖山森林土壤酸性磷酸单酯酶活性和有效磷含量的影响. 应用生态学报, 2011, 22(3): 631-636
[43] Hou E, Lu X, Jiang L, et al. Quantifying soil phosphorus dynamics: A data assimilation approach. Journal of Geophysical Research: Biogeosciences, 2019, 124: 2159-2173
[44] Qian X, Gu J, Sun W, et al. Changes in the soil nutrient levels, enzyme activities, microbial community function, and structure during apple orchard maturation. Applied Soil Ecology, 2014, 77: 18-25

氮添加对毛竹林土壤酸性磷酸单酯酶动力学参数的影响

Effects of nitrogen addition on the kinetic parameters of soil acid phosphomonoesterase in a Moso bamboo forest

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