杉木人工林土壤微生物生物量和碳源利用能力对模拟氮沉降和干旱的响应

doi:10.13287/j.1001-9332.202209.016

应用生态学报 ›› 2022, Vol. 33 ›› Issue (9): 2388-2396.doi: 10.13287/j.1001-9332.202209.016

杉木人工林土壤微生物生物量和碳源利用能力对模拟氮沉降和干旱的响应

宋鸽^1,2, 李晓杰^1,2, 王全成^1,2, 吕茂奎^1,2, 谢锦升^1,2,3, 贺纪正^1,2,3, 郑勇^1,2*

¹福建师范大学湿润亚热带生态地理过程教育部重点实验室, 福州 350007;
²福建师范大学地理科学学院, 福州 350007;
³福建三明森林生态系统国家野外科学观测研究站, 福建三明 365002

收稿日期:2022-01-29 接受日期:2022-07-14 出版日期:2022-09-15 发布日期:2023-03-15
通讯作者: * E-mail: zhengy@fjnu.edu.cn
作者简介:宋鸽, 女, 1997年生, 硕士研究生。主要从事土壤微生物生态学研究。E-mail: sg13156235062@163.com
基金资助:
国家自然科学基金项目(31971447)资助。

Responses of soil microbial biomass and carbon source utilization to simulated nitrogen deposition and drought in a Cunninghamia lanceolata plantation

SONG Ge^1,2, LI Xiao-jie^1,2, WANG Quan-cheng^1,2, LYU Mao-kui^1,2, XIE Jin-sheng^1,2,3, HE Ji-zheng^1,2,3, ZHENG Yong^1,2*

¹Key Laboratory for Humid Subtropical Eco-geographical Processes of the Ministry of Education, Fujian Normal University, Fuzhou 350007, China;
²School of Geographical Sciences, Fujian Normal University, Fuzhou 350007, China;
³Fujian Sanming Forest Ecosystem National Observation and Research Station, Sanming 365002, Fujian, China

Received:2022-01-29 Accepted:2022-07-14 Online:2022-09-15 Published:2023-03-15

摘要/Abstract

摘要： 杉木林是我国亚热带地区最主要的人工林和重要的碳汇。本研究以杉木人工林为对象,通过设置氮素添加(40 kg N·hm^-2·a^-1)和隔离降雨(-50%)试验分别模拟氮沉降和干旱,在夏季(7月)和冬季(1月)采集表层土壤,通过磷脂脂肪酸、平板计数、Biolog等方法综合分析土壤微生物生物量、数量及碳源利用能力。结果表明: 土壤微生物生物量及微生物组成在两季节间存在显著差异;氮添加和隔离降雨可在一定程度上减少可培养细菌和真菌的数量,细菌数量较真菌数量对氮添加和隔离降雨的响应更敏感。隔离降雨显著抑制了土壤中微生物的碳源利用能力,而氮添加无显著影响。土壤细菌数量与微生物碳源利用能力呈显著正相关,表明可培养细菌是介导微生物碳转化的关键组分。本研究强调了氮沉降和干旱对亚热带杉木人工林表层土壤微生物的影响,可为评估未来全球变化情景下亚热带森林生态系统的土壤微生物生态功能提供科学依据。

关键词: Biolog, CFUs, 碳源利用能力, 模拟氮沉降, 干旱, 磷脂脂肪酸

Abstract: Chinese fir (Cunninghamia lanceolata) plantation is a dominant forest type and carbon sink in the subtropical region in China. An experiment with simulated nitrogen deposition (addition of 40 kg N·hm^-2·a^-1) and drought (50% of precipitation exclusion, PE) was established in Chinese fir plantation in 2018. Soil samples (0-15 cm) were collected in summer (July 2020) and winter (January 2021). Soil microbial biomass, colony forming units (CFUs) and carbon source utilization were determined through phospholipid fatty acids (PLFAs), plate count, and Biolog methods, respectively. The results showed significant seasonal variations of PLFAs-related microbial biomass and composition. Soil bacterial and fungal CFUs tended to be decreased by nitrogen addition or precipitation exclusion treatment, and bacterial CFUs were more sensitive to the two treatments than fungal CFUs. Soil microbial function (i.e. carbon source utilization) was not affected by nitrogen addition, but significantly decreased by precipitation exclusion. There was a significant positive correlation between bacterial CFUs and microbial function, indicating the crucial roles of culturable bacteria in microbial carbon transformation. Our results highlight the critical effects of nitrogen deposition and 50% reduced precipitation on microbes in topsoil of fir plantation, with implications for unraveling soil microbial ecological function of subtropical forest ecosystem under global changes in future.

Key words: Biolog, CFUs, carbon source utilization, simulated nitrogen deposition, drought, PLFAs

宋鸽, 李晓杰, 王全成, 吕茂奎, 谢锦升, 贺纪正, 郑勇. 杉木人工林土壤微生物生物量和碳源利用能力对模拟氮沉降和干旱的响应[J]. 应用生态学报, 2022, 33(9): 2388-2396.

SONG Ge, LI Xiao-jie, WANG Quan-cheng, LYU Mao-kui, XIE Jin-sheng, HE Ji-zheng, ZHENG Yong. Responses of soil microbial biomass and carbon source utilization to simulated nitrogen deposition and drought in a Cunninghamia lanceolata plantation[J]. Chinese Journal of Applied Ecology, 2022, 33(9): 2388-2396.

参考文献

[1] Liu XJ, Zhang Y, Han WX, et al. Enhanced nitrogen deposition over China. Nature, 2013, 494: 459-462
[2] 付伟, 武慧, 赵爱花, 等. 陆地生态系统氮沉降的生态效应: 研究进展与展望. 植物生态学报, 2020, 44(5): 475-493
[3] Tian D, Niu S. A global analysis of soil acidification caused by nitrogen addition. Environmental Research Letters, 2015, 10: 024019
[4] Shi XZ, Hu HW, Wang JQ, et al. Niche separation of comammox Nitrospira and canonical ammonia oxidizers in an acidic subtropical forest soil under long-term nitrogen deposition. Soil Biology and Biochemistry, 2018, 126: 114-122
[5] Wang JQ, Shi XZ, Zheng CY, et al. Different responses of soil bacterial and fungal communities to nitrogen deposition in a subtropical forest. Science of the Total Environment, 2020, 755: 142449
[6] 李猛, 张恩平, 张淑红, 等. 长期不同施肥设施菜地土壤酶活性与微生物碳源利用特征比较. 植物营养与肥料学报, 2017, 23(1): 44-53
[7] Treseder KK. Nitrogen additions and microbial biomass: A meta-analysis of ecosystem studies. Ecology Letters, 2008, 11: 1111-1120
[8] Shao YH, Liu T, Eisenhauer N, et al. Plants mitigate detrimental nitrogen deposition effects on soil biodiversity. Soil Biology and Biochemistry, 2018, 127: 178-186
[9] 陈松鹤, 徐开未, 樊高琼, 等. 长期施氮对饲草玉米产量、土壤养分和微生物数量的影响. 四川农业大学学报, 2019, 37(3): 314-320
[10] Chen QL, Ding J, Li CY, et al. Microbial functional attributes, rather than taxonomic attributes, drive top soil respiration, nitrification and denitrification processes. Science of the Total Environment, 2020, 734: 139479
[11] Chen QL, Ding J, Zhu D, et al. Rare microbial taxa as the major drivers of ecosystem multifunctionality in long-term fertilized soils. Soil Biology and Biochemistry, 2020, 141: 107686
[12] 冯慧芳, 林婉奇, 薛立. 氮磷添加和栽植密度对大叶相思林土壤微生物群落功能多样性的影响. 生态学报, 2021, 41(6): 2305-2314
[13] Thomas RQ, Canham CD, Weathers KC, et al. Increased tree carbon storage in response to nitrogen deposition in the US. Nature Geoscience, 2010, 3: 13-17
[14] Lesk C, Rowhani P, Ramankutty N. Influence of extreme weather disasters on global crop production. Nature, 2016, 529: 84-87
[15] 周广胜, 何奇瑾. 生态系统响应全球变化的陆地样带研究. 地球科学进展, 2012, 27(5): 563-572
[16] Anderegg WRL, Trugman AT, Badgley G, et al. Divergent forest sensitivity to repeated extreme droughts. Nature Climate Change, 2020, 10: 1091-1095
[17] Luo WT, Griffin-Nolan RJ, Ma W, et al. Plant traits and soil fertility mediate productivity losses under extreme drought in C-3 grasslands. Ecology, 2021, 102: e03465
[18] Schimel JP. Life in dry soils: Effects of drought on soil microbial communities and processes. Annual Review of Ecology, Evolution, and Systematics, 2018, 49: 409-432
[19] Bastida F, Torres IF, Andrés-Abellán M, et al. Differential sensitivity of total and active soil microbial communities to drought and forest management. Global Change Biology, 2017, 23: 4185-4203
[20] Suseela V, Conant RT, Wallenstein MD, et al. Effects of soil moisture on the temperature sensitivity of heterotrophic respiration vary seasonally in an old-field climate change experiment. Global Change Biology, 2012, 18: 336-348
[21] Vicente-Serrano SM, Quiring SM, Peña-Gallardo M, et al. A review of environmental droughts: Increased risk under global warming? Earth-Science Reviews, 2020, 201: 102953
[22] 秦永梅, 杨慧, 李杰, 等. 干旱对樱桃幼苗根际微生物及生理指标的影响. 西部林业科学, 2019, 48(4): 44-49
[23] 孙欣. 半干旱草原微生物物种组成和功能基因对降水变化的响应. 硕士论文. 北京: 清华大学, 2015
[24] Sun LJ, Qi YC, Dong YS, et al. Interactions of water and nitrogen addition on soil microbial community composition and functional diversity depending on the inter-annual precipitation in a Chinese steppe. Journal of Integrative Agriculture, 2015, 14: 788-799
[25] Jensen KD, Beier C, Michelsen A, et al. Effects of experimental drought on microbial processes in two temperate heathlands at contrasting water conditions. Applied Soil Ecology, 2003, 24: 165-176
[26] Sun Y, Chen HYH, Jin L, et al. Drought stress induced increase of fungi:bacteria ratio in a poplar plantation. Catena, 2020, 193: 104607
[27] 吴华清, 陈小梅, 林媚珍, 等. 降水处理对南亚热带季风林土壤微生物群落结构的影响. 生态环境学报, 2016, 25(4): 583-590
[28] Su X, Su XL, Yang SC, et al. Drought changed soil organic carbon composition and bacterial carbon metabolizing patterns in a subtropical evergreen forest. Science of the Total Environment, 2020, 736: 139568
[29] 林婉奇, 薛立. 基于BIOLOG技术分析氮沉降和降水对土壤微生物功能多样性的影响. 生态学报, 2020, 40(12): 4188-4197
[30] 郑勇, 贺纪正. 森林土壤微生物对干旱和氮沉降的响应. 应用生态学报, 2020, 31(7): 2464-2472
[31] Ackerman D, Millet DB, Chen X. Global estimates of inorganic nitrogen deposition across four decades. Global Biogeochemical Cycles, 2019, 33: 100-107
[32] 占瑞芬, 孙国武, 赵兵科, 等. 中国东部副热带夏季风降水的准双周振荡及其可能维持机制. 高原气象, 2008, 27(增刊1): 98-108
[33] 余朝晖. 不同林龄杉木人工林生态功能比较研究. 林业勘察设计, 2018, 38(3): 6-14
[34] 张辉, 蔡一冰, 胡亚楠, 等. 目标树经营模式对杉木人工林生长及土壤肥力的短期影响. 西北林学院学报, 2022, 37(1): 191-197
[35] 李晓杰. 模拟降雨减少与氮沉降对杉木人工林土壤微生物及呼吸组分的影响. 博士论文. 福州: 福建师范大学, 2021
[36] Cheng SL, Fang HJ, Yu GR. Threshold responses of soil organic carbon concentration and composition to multi-level nitrogen addition in a temperate needle-broad-leaved forest. Biogeochemistry, 2018, 137: 219-233
[37] 中国科学院南京土壤研究所微生物室. 土壤微生物研究法. 北京: 科学出版社, 1985
[38] 鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科技出版社, 2000
[39] van Diepen LTA, Lilleskov EA, Pregitzer KS, et al. Simulated nitrogen deposition causes a decline of intra- and extraradical abundance of arbuscular mycorrhizal fungi and changes in microbial community structure in northern hardwood forests. Ecosystems, 2010, 13: 683-695
[40] 赵学超, 徐柱文, 刘圣恩, 等. 氮添加对多伦草原土壤微生物呼吸及其温度敏感性的影响. 生态学报, 2020, 40(5): 1551-1561
[41] Monkai J, Goldberg SD, Hyde KD, et al. Natural forests maintain a greater soil microbial diversity than that in rubber plantations in Southwest China. Agriculture, Ecosystems and Environment, 2018, 265: 190-197
[42] Huang SD, Ye GF, Lin J, et al. Autotrophic and heterotrophic soil respiration responds asymmetrically to drought in a subtropical forest in the Southeast China. Soil Biology and Biochemistry, 2018, 123: 242-249
[43] Williams A, de Vries FT. Plant root exudation under drought: Implications for ecosystem functioning. New Phytologist, 2020, 225: 1899-1905
[44] Högberg MN, Högberg P. Extramatrical ectomycorrhizal mycelium contributes one-third of microbial biomass and produces, together with associated roots, half the dissolved organic carbon in a forest soil. New Phytologist, 2002, 154: 791-795
[45] Deng Q, Hui DF, Zhang DQ, et al. Effects of precipitation increase on soil respiration: A three-year field experiment in subtropical forests in China. PLoS One, 2012, 7(7): e41493
[46] Zhou GY, Zhou XH, Liu RQ, et al. Soil fungi and fine root biomass mediate drought-induced reductions in soil respiration. Functional Ecology, 2022, 34: 2634-2643
[47] Wang Y, Liu SR, Luan JW, et al. Nitrogen addition exacerbates the negative effect of throughfall reduction on soil respiration in a bamboo forest. Forests, 2021, 12: 724
[48] 田雅楠, 王红旗. Biolog法在环境微生物功能多样性研究中的应用. 环境科学与技术, 2011, 34(3): 50-57
[49] Gensberger ET, Gössl EM, Antonielli L, et al. Effect of different heterotrophic plate count methods on the estimation of the composition of the culturable microbial community. PeerJ, 2015, 3: e862

杉木人工林土壤微生物生物量和碳源利用能力对模拟氮沉降和干旱的响应

Responses of soil microbial biomass and carbon source utilization to simulated nitrogen deposition and drought in a Cunninghamia lanceolata plantation

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