模拟氮沉降降低亚热带米槠天然林土壤氧化亚氮排放潜势

doi:10.13287/j.1001-9332.202210.005

应用生态学报 ›› 2022, Vol. 33 ›› Issue (10): 2705-2710.doi: 10.13287/j.1001-9332.202210.005

模拟氮沉降降低亚热带米槠天然林土壤氧化亚氮排放潜势

邓米林^1,2, 冯蒙蒙^1,2, 刘小飞^2,3, 陈仕东^2,3, 贺纪正^1,2, 林永新^1,2*

¹湿润亚热带山地生态国家重点实验室培育基地, 福州 350007;
²福建师范大学地理科学学院, 福州 350007;
³福建三明森林生态系统与全球变化国家野外科学观测研究站, 福建三明 365000

收稿日期:2022-04-01 修回日期:2022-05-30 出版日期:2022-10-15 发布日期:2023-04-15
通讯作者: * E-mail: yxlin@fjnu.edu.cn
作者简介:邓米林, 男, 1998年生, 硕士研究生。主要从事土壤微生物生态学研究。E-mail: 1550342935@qq.com
基金资助:
国家自然科学基金项目(41930756,42077041)和福建省自然科学基金项目(2020J01187)

Simulated nitrogen deposition reduces potential nitrous oxide emissions in a natural Castanopsis carlesii forest soil

DENG Mi-lin^1,2, FENG Meng-meng^1,2, LIU Xiao-fei^2,3, CHEN Shi-dong^2,3, HE Ji-zheng^1,2, LIN Yong-xin^1,2*

¹Cultivation Base of State Key Laboratory for Subtropical Mountain Ecology, Fuzhou 350007, China;
²School of Geographical Sciences, Fujian Normal University, Fuzhou 350007, China;
³Fujian Sanming Forest Ecosystem and Global Change National Observation and Research Station, Sanming 365000, Fujian, China

Received:2022-04-01 Revised:2022-05-30 Online:2022-10-15 Published:2023-04-15

摘要/Abstract

摘要： 我国亚热带地区大气氮沉降量逐年上升,对森林土壤生物地球化学循环造成严重影响。本研究设置了对照(不添加氮)、低氮(40 kg·hm^-2·a^-1)和高氮(80 kg·hm^-2·a^-1)处理,分析了亚热带米槠天然林土壤反硝化功能基因丰度和N₂O排放潜势对氮沉降的响应。结果表明: 高氮处理显著降低土壤N₂O排放潜势。长期(8年)氮沉降对nirS、nirK、nosZ Ⅰ和nosZ Ⅱ基因丰度均无显著影响,但nosZ Ⅰ丰度均显著高于nosZ Ⅱ丰度,表明nosZ Ⅰ在酸性森林土壤中占主导。与对照相比,高氮处理显著降低(nirK+nirS)/(nosZ Ⅰ+nosZ Ⅱ)值。(nirK+nirS)/(nosZ Ⅰ+nosZ Ⅱ)值与土壤pH值呈显著正相关。长期高氮沉降可能通过降低土壤pH值使得土壤(nirK+nirS)/(nosZ Ⅰ+nosZ Ⅱ)值下降,从而降低森林土壤N₂O排放潜势。

关键词: 氮添加, 森林土壤, N₂O排放, 反硝化基因

Abstract: The reactive nitrogen deposition in subtropical region of China has been increasing annually, which affects biogeochemical processes in forest soils. In this study, three treatments were established, including control (no N addition, CK), low nitrogen deposition (40 kg·hm^-2·a^-1, LN), and high nitrogen deposition (80 kg·hm^-2·a^-1, HN) to study the response of denitrifying functional genes and potential N₂O emissions to simulated nitrogen deposition in the soils of a natural Castanopsis carlesii forest. Results showed that HN significantly decreased soil potential N₂O emission, while 8-year nitrogen deposition did not affect the abundances of nirS, nirK, nosZ Ⅰ and nosZ Ⅱ. However, the abundance of nosZ Ⅰwas significantly higher than nosZ Ⅱ in all the treatments, indicating that nosZ Ⅰ dominated over nosZ Ⅱ in the acidic soils. HN significantly decreased the ratio of (nirK+nirS)/(nosZ Ⅰ+nosZ Ⅱ), which was positively correlated with soil pH. The results suggested that long-term high nitrogen deposition reduced soil pH and the abundance ratio of (nirK+nirS)/(nosZ Ⅰ+nosZ Ⅱ), which subsequently reduced the potential N₂O emission.

Key words: nitrogen addition, forest soil, N₂O emission, denitrifying gene

邓米林, 冯蒙蒙, 刘小飞, 陈仕东, 贺纪正, 林永新. 模拟氮沉降降低亚热带米槠天然林土壤氧化亚氮排放潜势[J]. 应用生态学报, 2022, 33(10): 2705-2710.

DENG Mi-lin, FENG Meng-meng, LIU Xiao-fei, CHEN Shi-dong, HE Ji-zheng, LIN Yong-xin. Simulated nitrogen deposition reduces potential nitrous oxide emissions in a natural Castanopsis carlesii forest soil[J]. Chinese Journal of Applied Ecology, 2022, 33(10): 2705-2710.

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模拟氮沉降降低亚热带米槠天然林土壤氧化亚氮排放潜势

Simulated nitrogen deposition reduces potential nitrous oxide emissions in a natural Castanopsis carlesii forest soil

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