超声波对铜绿微囊藻与蛋白核小球藻生理特征及竞争生长的影响

doi:10.13287/j.1001-9332.202210.028

应用生态学报 ›› 2022, Vol. 33 ›› Issue (10): 2845-2852.doi: 10.13287/j.1001-9332.202210.028

超声波对铜绿微囊藻与蛋白核小球藻生理特征及竞争生长的影响

谭啸¹, 徐杨雪¹, 李聂贵², 段志鹏^1,3*, 蒋瑀霁⁴, 曾庆飞⁵, 强娟⁶

¹河海大学环境学院, 浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098;
²水利部南京水利水文自动化研究所, 南京 210012;
³河海大学水文水资源学院, 南京 210098;
⁴中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室, 南京 210008;
⁵中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008;
⁶常州市武进区水利局, 江苏常州 213115

收稿日期:2021-09-02 修回日期:2022-03-03 出版日期:2022-10-15 发布日期:2023-04-15
通讯作者: * E-mail: hhualgae@163.com
作者简介:谭啸, 男, 1980年生, 博士, 教授。主要从事藻类生态学与藻华防控研究。E-mail: hhualgae@163.com
基金资助:
国家自然科学基金项目(32071569)、2020年度中国科学院科研仪器设备研制项目(YJKYYQ20200048)、中央高校科研业务费(B210202010)和中国博士后基金(2020M681472)

Effects of ultrasound on the physiological characteristics and competitive growth between Microcystis aeruginosa and Chlorella pyrenoidosa

TAN Xiao¹, XU Yang-xue¹, LI Nie-gui², DUAN Zhi-peng^1,3*, JIANG Yu-ji⁴, ZENG Qing-fei⁵, QIANG Juan⁶

¹Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing 210098, China;
²Nanjing Institute of Water Conservancy and Hydrology Automation, Ministry of Water Resources, Nanjing 210012, China;
³College of Hydrology and Water Resources, Hohai University, Nanjing 210098, China;
⁴State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;
⁵State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China;
⁶Water Resources Bureau of Wujin District in Changzhou City, Changzhou 213115, Jiangsu, China

Received:2021-09-02 Revised:2022-03-03 Online:2022-10-15 Published:2023-04-15

摘要/Abstract

摘要： 铜绿微囊藻是常见的水华蓝藻,常常在湖泊中与蛋白核小球藻共存或竞争生长。超声波可用于藻华即时治理,能够降低藻类生理活性,影响藻类生长,还可能改变藻类种间竞争关系。为了探究超声胁迫(35 kHz,0.035 W·cm^-3)对铜绿微囊藻与蛋白核小球藻的生理特征及种间竞争的影响,本研究设置纯藻组和1:1混合组(细胞浓度比)进行试验。结果表明: 铜绿微囊藻对超声胁迫更加敏感。超声处理600 s后,铜绿微囊藻的光合活性(F_v/F_m)和酯酶活性存在显著变化,纯藻组和混合组的F_v/F_m分别降低了51.8%和64.7%。而各组中蛋白核小球藻的光合活性变化较小。同时,铜绿微囊藻释放的荧光溶解性有机物(类色氨酸、类酪氨酸、类富里酸物质)含量多于蛋白核小球藻。两种藻的细胞浓度对超声波的响应也不同,蛋白核小球藻变化较小,而铜绿微囊藻的细胞浓度出现不同程度的下降。尤其是600 s超声处理大幅降低了混合组中铜绿微囊藻的细胞浓度(-42.6%),在超声胁迫解除后的8 d内蛋白核小球藻占优势,种间关系由铜绿微囊藻单边抑制蛋白核小球藻转变为两者互相抑制。在超声处理后,铜绿微囊藻的活性能够逐渐恢复,为了提高控藻效果的持久性,建议在一周后再次进行超声处理。

关键词: 超声波, 铜绿微囊藻, 蛋白核小球藻, 生理特性, 种间竞争, 水华

Abstract: Microcystis aeruginosa is a common bloom-forming cyanobacterium, which generally coexists and competes with Chlorella pyrenoidosa in lakes. Sonication can be used for emergency management of algal blooms. Ultrasound influences algal growth and physiological parameters, as well as interspecific competition in algal community. To explore the effects of ultrasonic stress (35 kHz, 0.035 W·cm^-3) on physiological characteristics and interspecific competition of algae, M. aeruginosa and C. pyrenoidosa were sonicated in mono- and co-cultures (1:1 mixture, according to cell concentration). Results showed that M. aeruginosa was more sensitive to ultrasonic stress. After the sonication for 600 s, both photosynthetic activity (F_v/F_m) and esterase activity of M. aeruginosa showed significant changes, with F_v/F_m values in mono- and co-cultures being decreased by 51.8% and 64.7%, respectively. In comparison, F_v/F_m values of C. pyrenoidosa changed slightly. M. aeruginosa released more chromophoric dissolved organic matter (CDOM, including tryptophan-, tyrosine-, and fulvic-like substances) than C. pyrenoidosa. The cell concentration of C. pyrenoidosa showed little changes regardless of sonication time, while the cell concentration of M. aeruginosa decreased at different degrees. The cell concentration of M. aeruginosa in co-cultures decreased by 42.6% after sonication for 600 s, which might be responsible for the dominance of C. pyrenoidosa during 8 days after sonication. M. aeruginosa inhibited C. pyrenoidosa in other treatments, but mutual inhibition appeared in the 600 s sonication treatment. After ultrasonic treatment, the activity of M. aeruginosa could recover gradually. The treatment should be conducted again within a week to improve the persistence of algal control.

Key words: ultrasound, Microcystis aeruginosa, Chlorella pyrenoidosa, physiological characteristic, interspecific competition, algal bloom

谭啸, 徐杨雪, 李聂贵, 段志鹏, 蒋瑀霁, 曾庆飞, 强娟. 超声波对铜绿微囊藻与蛋白核小球藻生理特征及竞争生长的影响[J]. 应用生态学报, 2022, 33(10): 2845-2852.

TAN Xiao, XU Yang-xue, LI Nie-gui, DUAN Zhi-peng, JIANG Yu-ji, ZENG Qing-fei, QIANG Juan. Effects of ultrasound on the physiological characteristics and competitive growth between Microcystis aeruginosa and Chlorella pyrenoidosa[J]. Chinese Journal of Applied Ecology, 2022, 33(10): 2845-2852.

参考文献

[1] 徐春燕, 俞秋佳, 徐凤洁, 等. 淀山湖浮游植物优势种生态位. 应用生态学报, 2012, 23(9): 2550-2558
[2] Nakano K, Lee TJ, Matsumura M. In situ algal bloom control by the integration of ultrasonic radiation and jet circulation to flushing. Environmental Science & Techno-logy, 2001, 35: 4941-4946
[3] 韩景明. 澎溪河水环境及超声波除(抑)藻技术研究. 硕士论文. 重庆: 重庆大学, 2011
[4] Peng YZ, Zhang Z, Kong Y, et al. Effects of ultrasound on Microcystis aeruginosa cell destruction and release of intracellular organic matter. Ultrasonics Sonochemistry, 2020, 63: 104909
[5] 万莉, 邵路路, 陆开宏, 等. 超声波对铜绿微囊藻超微结构和生理特性的影响. 水生生物学报, 2014, 38(3): 516-524
[6] Kong Y, Peng YZ, Zhang Z, et al. Removal of Microcystis aeruginosa by ultrasound: Inactivation mechanism and release of algal organic matter. Ultrasonics Sonochemistry, 2019, 56: 447-457
[7] Rajasekhar P, Fan LH, Nguyen T, et al. Impact of soni-cation at 20 kHz on Microcystis aeruginosa, Anabaena circinalis and Chlorella sp. Water Research, 2012, 46: 1473-1481
[8] Duan ZP, Tan X, Li NG. Ultrasonic selectivity on depressing photosynthesis of cyanobacteria and green algae probed by chlorophyll-a fluorescence transient. Water Science and Technology, 2017, 76: 2085-2094
[9] Song H, Lavoie M, Fan XJ, et al. Allelopathic interactions of linoleic acid and nitric oxide increase the competitive ability of Microcystis aeruginosa. The ISME Journal, 2017, 11: 1865-1876
[10] Rajasekhar P, Fan LH, Nguyen T, et al. A review of the use of sonication to control cyanobacterial blooms. Water Research, 2012, 46: 4319-4329
[11] 谭啸, 孙玉童, 段志鹏, 等. 不同超声强度下微囊藻群体沉降及其上浮过程对光照和温度的响应. 湖泊科学, 2017, 29(5): 1168-1176
[12] Tan X, Dai KW, Parajuli K, et al. Effects of phenolic pollution on interspecific competition between Microcystis aeruginosa and Chlorella pyrenoidosa and their photosynthetic responses. International Journal of Environmental Research and Public Health, 2019, 16: 3947-3958
[13] Qu JQ, Shen LP, Zhao M, et al. Determination of the role of Microcystis aeruginosa in toxin generation based on phosphoproteomic profiles. Toxins, 2018, 10: 304
[14] 谭啸, 段志鹏, 李聂贵, 等. 超声波处理对微囊藻群体光合活性和沉降过程的影响. 湖泊科学, 2017, 29(6): 1324-1330
[15] Mason TJ, Lorimer JP, Bates DM. Quantifying sonochemistry: Casting some light on a ‘black art'. Ultrasonics, 1992, 30: 40-42
[16] Stirbet A, Govindjee. On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and Photosystem Ⅱ: Basics and applications of the OJIP fluorescence transient. Journal of Photochemistry and Photobiology B: Biology, 2011, 104: 236-257
[17] Geary S, Ganf G, Brookes J. The use of FDA and flow cytometry to measure the metabolic activity of the cyanobacteria, Microcystis aeruginosa. International Association of Theoretical and Applied Limnology-Proceedings, 2017, 26: 2367-2369
[18] Xu HC, Cai HY, Yu GH, et al. Insights into extracellular polymeric substances of cyanobacterium Microcystis aeruginosa using fractionation procedure and parallel factor analysis. Water Research, 2013, 47: 2005-2014
[19] 汪芳, 葛蔚, 柴超, 等. 不同营养条件对东海原甲藻和中肋骨条藻竞争参数的影响. 应用生态学报, 2012, 23(5): 1393-1399
[20] Stedmon C, Bro R. Characterizing dissolved organic matter fluorescence with parallel factor analysis: A tutorial. Limnology and Oceanography: Methods, 2008, 6: 572-579
[21] 周丽, 付子轼, 陈桂发, 等. 陆生植物化感抑制铜绿微囊藻作用效应及机制研究进展. 应用生态学报, 2018, 29(5): 1715-1724
[22] 段永平. 叶绿体类囊体膜光系统捕光及反应中心叶绿素蛋白复合体的结构与功能. 赤峰学院学报: 自然科学版, 2005, 21(5): 35-36
[23] Wang R, Hua M, Yu Y, et al. Evaluating the effects of allelochemical ferulic acid on Microcystis aeruginosa by pulse-amplitude-modulated (PAM) fluorometry and flow cytometry. Chemosphere, 2016, 147: 264-271
[24] 孔媛. 超声去除铜绿微囊藻技术参数的多目标优化及失活藻细胞特性研究. 硕士论文. 重庆: 重庆大学, 2019
[25] Perron MC, Juneau P. Effect of endocrine disrupters on photosystem Ⅱ energy fluxes of green algae and cyanobacteria. Environmental Research, 2011, 111: 520-529
[26] Tang JW, Wu QY, Hao HW, et al. Effect of 1.7 MHz ultrasound on a gas-vacuolate cyanobacterium and a gas-vacuole negative cyanobacterium. Colloids and Surfaces B: Biointerfaces, 2004, 36: 115-121
[27] 张英, 董绍华. 氨基酸清除活性氧自由基作用的研究. 科技通报, 1997, 13(5): 33-36
[28] Duan ZP, Tan X, Guo JJ, et al. Effects of biological and physical properties of microalgae on disruption induced by a low-frequency ultrasound. Journal of Applied Phycology, 2017, 29: 2937-2946
[29] Tan X, Zhang DF, Duan ZP, et al. Effects of light color on interspecific competition between Microcystis aeruginosa and Chlorella pyrenoidosa in batch experiment. Environmental Science and Pollution Research, 2019, 27: 344-352
[30] 刘晓娟, 段舜山, 李爱芬. 绿色巴夫藻受紫外(UV-B)胁迫后的超补偿生长效应. 应用生态学报, 2007, 18(1): 169-173
[31] 张全兴. 超声波非均匀介质传播衰减特性研究. 硕士论文. 沈阳: 沈阳工业大学, 2015
[32] Li J, Ou DY, Zheng LL, et al. Applicability of the fluorescein diacetate assay formetabolic activity measurement of Microcystis aeruginosa (Chroococcales, Cyanobacteria). Phycological Research, 2011, 59: 200-207
[33] Babica P, Blaha L, Marsálek B. Exploring the natural role of microcystins: A review of effects on photoautotrophic organisms. Journal of Phycology, 2006, 42: 9-20
[34] 李婷, 景元书, 韩玮, 等. 亚高温胁迫解除后铜绿微囊藻的生长恢复. 应用生态学报, 2014, 25(11): 3337-3343

超声波对铜绿微囊藻与蛋白核小球藻生理特征及竞争生长的影响

Effects of ultrasound on the physiological characteristics and competitive growth between Microcystis aeruginosa and Chlorella pyrenoidosa

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	钱必长, 赵晨, 赵继浩, 赖华江, 李向东, 刘兆新. 不同花生棉花间作模式对花生生育后期生理特性及产量的影响 [J]. 应用生态学报, 2022, 33(9): 2422-2430.
[2]	邱雨, 王江南, 马增岭, 陈宇涛, 张子怡, 王敏. 铜锈环棱螺对水华中常见藻类的抑制效应 [J]. 应用生态学报, 2022, 33(10): 2853-2861.
[3]	胡小京, 敖飞雄, 石乐娟, 彭强, 李欣, 周小会, 陈孝玉龙. 两株链霉菌对非洲菊生长和生理生化指标的影响 [J]. 应用生态学报, 2021, 32(9): 3321-3326.
[4]	申思, 郭文锋, 王伟, 李晓琼. 地上-地下植食性天敌对入侵植物空心莲子草与本地种莲子草种间关系的影响 [J]. 应用生态学报, 2021, 32(8): 2975-2981.
[5]	朱娟娟, 马海军, 张琇, 李敏, 徐小云, 覃洪云. 盐胁迫下解钾菌对枸杞幼苗的促生效应 [J]. 应用生态学报, 2021, 32(4): 1289-1297.
[6]	刘坤, 俞存根, 许永久, 江新琴, 郑基, 于南京, 张佩怡, 蒋巧丽, 牛威震, 王慧君. 浙江披山海域主要鱼类时空生态位 [J]. 应用生态学报, 2021, 32(3): 1069-1079.
[7]	杜澜, 夏捷, 李海花, 吴炜, 陈亮, 张玮, 陈胜, 谢锦忠. 逐步失水过程中绿竹光响应进程及其拟合 [J]. 应用生态学报, 2019, 30(6): 2011-2020.
[8]	曹娜, 熊强强, 陈小荣, 贺浩华, 朱昌兰, 傅军如, 才硕, 徐涛. 不同施氮量对晚稻抵御抽穗扬花期低温的影响 [J]. 应用生态学报, 2018, 29(8): 2566-2574.
[9]	柳旭,陈钊,刘倩,高娅妮,周文楠,崔雪雯,王佺珍. 超声波对3种老化牧草种子萌发和幼苗生长的影响 [J]. 应用生态学报, 2018, 29(6): 1857-1866.
[10]	周丽, 付子轼, 陈桂发, 潘琦, 宋祥甫, 邹国燕, 刘娅琴. 陆生植物化感抑制铜绿微囊藻作用效应及机制研究进展 [J]. 应用生态学报, 2018, 29(5): 1715-1724.
[11]	陈士超, 王猛, 汪季, 高永, 刘宗奇, 王香. 紫花苜蓿种子萌发及幼苗生理特性对PEG6000模拟渗透势的响应 [J]. 应用生态学报, 2017, 28(9): 2923-2931.
[12]	卜祥祺, 刘琳, 穆亚楠, 官玉婷, 李红丽, 于飞海. 水位波动对南美蟛蜞菊和蟛蜞菊种内及种间关系的影响 [J]. 应用生态学报, 2017, 28(3): 797-804.
[13]	曹娜, 陈小荣, 贺浩华, 朱昌兰, 才硕, 徐涛, 谢亨旺, 刘方平. 抽穗扬花期不同灌水处理对晚稻抵御低温、产量和生理特性的影响 [J]. 应用生态学报, 2017, 28(12): 3935-3944.
[14]	姚海梅, 李永生, 张同祯, 赵娟, 王婵, 王汉宁, 方永丰. 旱-盐复合胁迫对玉米种子萌发和生理特性的影响 [J]. 应用生态学报, 2016, 27(7): 2301-2307.
[15]	王凯, 雷虹, 刘建华. 春季辽宁西北部主要绿化树种根叶抗旱生理性状评价 [J]. 应用生态学报, 2016, 27(6): 1853-1860.