闽江河口互花米草不同入侵阶段湿地土壤nirK型反硝化微生物群落结构及多样性

doi:10.13287/j.1001-9332.202211.016

应用生态学报 ›› 2022, Vol. 33 ›› Issue (11): 3007-3015.doi: 10.13287/j.1001-9332.202211.016

闽江河口互花米草不同入侵阶段湿地土壤nirK型反硝化微生物群落结构及多样性

陈冰冰^1,2, 孙志高^1,2,3*, 胡星云^1,2, 武慧慧^1,2, 王晓颖^1,2, 毛立^1,2, 厉彦哲^1,2

¹福建师范大学湿润亚热带生态地理过程教育部重点实验室, 福州 350007;
²福建师范大学福建省亚热带资源与环境重点实验室, 福州 350007;
³福建师范大学地理研究所, 福州 350007

收稿日期:2022-01-09 修回日期:2022-09-01 出版日期:2022-11-15 发布日期:2023-05-15
通讯作者: *E-mail: zhigaosun@163.com
作者简介:陈冰冰, 女, 1993年生, 博士研究生。主要从事河口湿地生物地球化学循环研究。E-mail: bingbingchen93@163.com
基金资助:
国家自然科学基金项目(41971128)、福建省“闽江学者奖励计划”项目和福建师范大学地理科学学院研究生科研创新基金项目

Structure and diversity of nirK-type denitrifying microbial community in marsh soils at different invasion stages of Spartina alterniflora in the Minjiang River estuary, China.

CHEN Bing-bing^1,2, SUN Zhi-gao^1,2,3*, HU Xing-yun^1,2, WU Hui-hui^1,2, WANG Xiao-ying^1,2, MAO Li^1,2, LI Yan-zhe^1,2

¹Ministry of Education Key Laboratory of Humid Subtropical Eco-geographical Process, Fujian Normal University, Fuzhou 350007, China;
²Fujian Provincial Key Laboratory for Subtropical Resources and Environment, Fujian Normal University, Fuzhou 350007, China;
³Institute of Geography, Fujian Normal University, Fuzhou 350007, China

Received:2022-01-09 Revised:2022-09-01 Online:2022-11-15 Published:2023-05-15

摘要/Abstract

摘要： 为了明确闽江河口互花米草海向不同入侵阶段湿地土壤nirK型反硝化微生物的群落结构及多样性,在鳝鱼滩东部的互花米草分布区,由陆向海方向选择互花米草海向入侵前的光滩(MF)、入侵1～2年(SAN)和入侵6～7年的互花米草(SA)湿地为研究对象,基于高通量测序技术,测定并分析了不同互花米草入侵阶段湿地土壤nirK型反硝化微生物群落结构及多样性的差异。结果表明:互花米草海向入侵降低了土壤中nirK型反硝化微生物群落的多样性及丰富度。不同入侵阶段湿地土壤的nirK型反硝化微生物主要包括变形菌门和放线菌门,其中变形菌门占绝对优势地位。互花米草海向入侵整体改变了湿地土壤中nirK型反硝化菌属的组成特征,MF、SAN、SA湿地土壤中相对丰度最高的nirK基因菌属分别为慢生根瘤菌属、中慢生根瘤菌属和产碱杆菌属。互花米草海向入侵增加了土壤中nirK型反硝化微生物群落组成的空间异质性,尤其是在SAN样地,这主要与样地本身的环境扰动性较大及互花米草海向入侵加大了样地环境因子的空间异质性有关。互花米草海向入侵主要是通过显著改变土壤理化因子(粒度组成、pH值和含水量)和氮养分条件(全氮、NH₄⁺-N、NO₃^--N)来影响nirK型反硝化微生物的群落结构及多样性。本研究结果有助于揭示互花米草海向入侵对湿地土壤反硝化过程影响的微生物机制。

关键词: nirK, 高通量测序, 微生物多样性, 群落结构, 互花米草, 闽江口

Abstract: To explore the differences in structure and diversity of nirK-type denitrifying microbial community in marsh soils at different invasion stages of Spartina alterniflora, the mudflat (MF, before invasion) and the S. alterniflora marsh after seaward invasion for 1-2 years (SAN) and 6-7 years (SA) in Shanyutan of the Minjiang River estuary were investigated by high-through put sequencing method. Results showed that the seaward invasion of S. alterniflora reduced the richness and diversity of nirK-type denitrifying microbial community in marsh soils. The nirK-type denitrifying microbial community in soils at different invasion stages included Proteobacteria and Actinobacteria, with Proteobacteria as the dominant one. The seaward invasion of S. alterniflora greatly altered the composition of nirK-type denitrifying microbial community in marsh soils. The highest relative abundance of genus in soils from different invasion stages were Bradyrhizobium, Mesorhizobium and Alcaligenes, respectively. The seaward invasion of S. alterniflora increased the spatial heterogeneity of nirK-type denitrifying microbial community composition in marsh soils. In SAN plot, the enhancement of spatial heterogeneity was primarily due to higher environmental disturbances in plots and the increased spatial heterogeneity of environmental variables caused by the seaward invasion of S. alterniflora. The seaward invasion of S. alterniflora altered the physico-chemical properties (e.g., grain composition, pH and moisture) and N nutrient conditions (total N, NH₄⁺-N and NO₃^--N) in marsh soils, which greatly altered the structure and diversity of nirK-type denitrifying microbial community. Our findings reveal the microbial mechanism of denitrification process in marsh soils during the seaward invasion of S. alterniflora.

Key words: nirK, high-throughput sequencing, microbial diversity, community structure, Spartina alterniflora, Minjiang River estuary

陈冰冰, 孙志高, 胡星云, 武慧慧, 王晓颖, 毛立, 厉彦哲. 闽江河口互花米草不同入侵阶段湿地土壤nirK型反硝化微生物群落结构及多样性[J]. 应用生态学报, 2022, 33(11): 3007-3015.

CHEN Bing-bing, SUN Zhi-gao, HU Xing-yun, WU Hui-hui, WANG Xiao-ying, MAO Li, LI Yan-zhe. Structure and diversity of nirK-type denitrifying microbial community in marsh soils at different invasion stages of Spartina alterniflora in the Minjiang River estuary, China.[J]. Chinese Journal of Applied Ecology, 2022, 33(11): 3007-3015.

参考文献

[1] Giblin AE, Wieder RK. Sulfur cycling in marine and freshwater wetlands// Howarth RV, Stewart JWB, Ivanou MV, eds. Sulfur Cycle in the Continents, Wetlands, Terrestrial Ecosystems and Associated Water Bodies. Chichester: John Wiley and Sons, 1992: 85-124
[2] Gomes J, Khandeparker R, Bandekar M, et al. Quantitative analyses of denitrifying bacterial diversity from a seasonally hypoxic monsoon governed tropical coastal region. Deep Sea Research Part Ⅱ: Topical Studies in Oceanography, 2018, 156: 34-43
[3] Jones CM, Hallin S. Ecological and evolutionary factors underlying global and local assembly of denitrifier communities. The ISME Journal, 2010, 4: 633-641
[4] 杨雪琴, 连英丽, 颜庆云, 等. 滨海湿地生态系统微生物驱动的氮循环研究进展. 微生物学报, 2018, 58(4): 633-648
[5] Mosier AC, Francis CA. Denitrifier abundance and activity across the San Francisco Bay estuary. Environmental Microbiology Reports, 2010, 2: 667-676
[6] Gao J, Hou LJ, Zheng YL, et al. nirS-encoding denitrifier community composition, distribution, and abundance along the coastal wetlands of China. Applied Microbiology and Biotechnology, 2016, 100: 8573-8582
[7] Desnues C, Michotey VD, Wieland A, et al. Seasonal and diel distributions of denitrifying and bacterial communities in a hypersaline microbial mat (Camargue, France). Water Research, 2007, 41: 3407-3419
[8] Zhang XL, Agogué H, Dupuy C, et al. Relative abundance sediments of hyper-nutrified estuarine tidal flats and in relation to environmental conditions. CLEAN-Soil, Air, Water, 2014, 42: 815-823
[9] Braker G, Zhou J, Wu L, et al. Nitrite reductase genes (nirK and nirS) as functional markers to investigate diversity of denitrifying bacteria in Pacific northwest marine sediment communities. Applied and Environmental Microbiology, 2000, 66: 2096-2104
[10] Sun ZG, Sun WG, Tong C, et al. China's coastal wetlands: Conservation history, implementation efforts, existing issues and strategies for future improvement. Environment International, 2015, 79: 25-41
[11] He CQ, Wang XX, Wang DY, et al. Impact of Spartina alterniflora invasion on soil bacterial community and associated greenhouse gas emission in the Jiuduansha wetland of China. Applied Soil Ecology, 2021, 168: 104168
[12] Shao TY, Zhao JJ, Liu AH, et al. Effects of soil physicochemical properties on microbial communities in different ecological niches in coastal area. Applied Soil Ecology, 2020, 150: 103486
[13] Chen J, Nie YX, Liu W, et al. Ammonia-oxidizing archaea are more resistant than denitrifiers to seasonalprecipitation changes in an acidic subtropical forest soil. Frontiers in Microbiology, 2017, 8: 1384
[14] 陈秀波, 段文标, 陈立新, 等. 小兴安岭3种原始红松混交林土壤nirK型反硝化微生物群落特征. 南京林业大学学报: 自然科学版, 2021, 45(2): 77-86
[15] Tang YQ, Zhang XY, Li DD, et al. Impacts of nitrogen and phosphorus additions on the abundance and community structure of ammonia oxidizers and denitrifying bacteria in Chinese fir plantations. Soil Biology and Biochemistry, 2016, 103: 284-293
[16] Liang YQ, Wu CF, Wei XM, et al. Characterization of nirS- and nirK-containing communities and potential denitrification activity in paddy soil from eastern China. Agriculture, Ecosystems and Environment, 2021, 319: 107561
[17] Wang N, Zhao YH, Yu JG, et al. Roles of bulk and rhizosphere denitrifying bacteria in denitrification from paddy soils under straw return condition. Journal of Soils and Sediments, 2021, 21: 2179-2191
[18] 陈泽斌, 李冰, 林丽, 等. nirS和nirK型反硝化细菌在水稻根中的分布. 东北农业科学, 2021, 46(10): 62-67
[19] Keil D, Meyer A, Berner D, et al. Influence of land-use intensity on the spatial distribution of N-cycling microorganisms in grassland soils. FEMS Microbiology Ecology, 2011, 77: 95-106
[20] 马秀艳, 蒋磊, 宋艳宇, 等. 温度和水分变化对冻土区泥炭地土壤氮循环功能基因丰度的影响. 生态学报, 2021, 41(17): 6707-6717
[21] Li FG, Li MC, Shi WC, et al. Distinct distribution patterns of proteobacterial nirK- and nirS-type denitrifiers in the Yellow River estuary, China. Canadian Journal of Microbiology, 2017, 63: 708-718
[22] 明红霞, 陈泉睿, 史银银, 等. 辽河口沉积物反硝化过程研究——以反硝化功能基因丰度及nirK型细菌群落结构分析为例. 海洋学报, 2020, 42(12): 82-92
[23] Ming HX, Fan JF, Chen QR, et al. Diversity and abundance of denitrifying bacteria in the sediment of a eutrophic estuary. Geomicrobiology Journal, 2021, 38: 199-209
[24] 王鹏, 吴莹, 刘素美, 等. 长江口外低氧区及其邻近海域表层沉积物反硝化微生物多样性和分布特征. 微生物学报, 2021, 61(6): 1474-1487
[25] Zhang WL, Zeng CS, Tong C, et al. Analysis of the expanding process of the Spartina alterniflora salt marsh in Shanyutan wetland, Minjiang River estuary by remote sensing. Procedia Environmental Sciences, 2011, 10: 2472-2477
[26] 鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科技出版社, 2000
[27] Adair KL, Lindgreen S, Poole AM, et al. Above- and belowground community strategies respond to different global change drivers. Scientific Reports, 2019, 9: 2540
[28] 霍玉珠, 王银华, 王春萍, 等. 互花米草入侵对天津滨海湿地土壤可培养细菌和真菌群落组成及多样性的影响. 天津师范大学学报: 自然科学版, 2021, 41(1): 34-41
[29] Knowles R. Denitrification. Microbiological Reviews, 1982, 46: 43-70
[30] Cheng Y, Wang J, Wang SQ, et al. Effects of soil moisture on gross N transformations and N₂O emission in acid subtropical forest soils. Biology and Fertility of Soils, 2014, 50: 1099-1108
[31] Ligi T, Truu M, Truu J, et al. Effects of soil chemical characteristics and water regime on denitrification genes (nirS, nirK, and nosZ) abundances in a created rive-rine wetland complex. Ecological Engineering, 2014, 72: 47-55
[32] Yang W, Jeelani N, Zhu ZH, et al. Alterations in soil bacterial community in relation to Spartina alterniflora Loisel. invasion chronosequence in the eastern Chinese coastal wetlands. Applied Soil Ecology, 2019, 135: 38-43
[33] 陈垚, 程启洪, 郑爽, 等. 干湿交替对生物滞留系统中氮素功能微生物群落的影响. 微生物学报, 2020, 60(3): 533-544
[34] Fierer N, Bradford MA, Jackson RB. Toward an ecolo-gical classification of soil bacteria. Ecology, 2007, 88: 1354-1364
[35] Feng MM, Adams JM, Fan KK, et al. Long-term fertilization influences community assembly processes of soil diazotrophs. Soil Biology and Biochemistry, 2018, 126: 151-158
[36] 曹乾斌, 王邵军, 陈闽昆, 等. 不同恢复阶段热带森林土壤nirS型反硝化微生物群落结构及多样性特征. 生态学报, 2021, 41(2): 626-636
[37] Pett-Ridge J, Petersen DG, Nuccio E, et al. Influence of oxic/anoxic fluctuations on ammonia oxidizers and nitrification potential in a wet tropical soil. FEMS Microbiology Ecology, 2013, 85: 179-194
[38] Yang W, Yan YE, Jiang F, et al. Response of the soil microbial community composition and biomass to a short-term Spartina alterniflora invasion in a coastal wetland of eastern China. Plant and Soil, 2016, 408: 443-456
[39] Xia F, Zeleke J, Sheng Q, et al. Communities of ammonia oxidizers at different stages of Spartina alterniflora invasion in salt marshes of Yangtze River estuary. Journal of Microbiology, 2015, 53: 311-320

闽江河口互花米草不同入侵阶段湿地土壤nirK型反硝化微生物群落结构及多样性

Structure and diversity of nirK-type denitrifying microbial community in marsh soils at different invasion stages of Spartina alterniflora in the Minjiang River estuary, China.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	黄睿智, 王奇, 孙婧依, 杨绍微, 赵倚霈, 刘建锋, 肖文发. 太白山南北坡栎类林物种组成与群落特征比较 [J]. 应用生态学报, 2023, 34(8): 2055-2064.
[2]	黄庆阳, 谢立红, 曹宏杰, 王立民, 杨帆, 王继丰, 刘赢男, 倪红伟. 细菌对五大连池火山森林凋落物早期分解的影响 [J]. 应用生态学报, 2023, 34(7): 1941-1948.
[3]	郭蓉, 吴旭东, 王占军, 蒋齐, 俞鸿千, 贺婧, 刘文娟, 马琨. 荒漠草原土壤细菌和真菌群落对降水变化的响应 [J]. 应用生态学报, 2023, 34(6): 1500-1508.
[4]	刘珊珊, 王全成, 史加勉, 刘子恺, 沈菊培, 贺纪正, 郑勇. 亚热带森林菌根植物根系真菌群落结构对氮磷添加的响应 [J]. 应用生态学报, 2023, 34(6): 1547-1554.
[5]	丁爽, 魏圣钊, 陈真亮, 邵婧, 段逢瑞, 严禹, 段兴武. 中国西南典型森林土壤微生物在不同土壤深度下的变化特征 [J]. 应用生态学报, 2023, 34(3): 614-622.
[6]	余安卫, 胡汶廷, 吴思颖, 尹海锋, 范川, 李贤伟. 目标树经营对马尾松人工林不同土层深度土壤线虫群落结构的影响 [J]. 应用生态学报, 2023, 34(2): 359-368.
[7]	江尚焘, 栗晗, 彭海英, 梅新兰, 陈廷速, 徐阳春, 董彩霞, 沈其荣. 有机肥替代部分化肥对芒果丛枝菌根真菌群落的影响 [J]. 应用生态学报, 2023, 34(2): 481-490.
[8]	邓米林, 叶桂萍, 胥超, 宛颂, 贺纪正, 林永新. 天然林转人工林对亚热带森林土壤团聚体中亚硝酸盐还原基因丰度的影响 [J]. 应用生态学报, 2023, 34(1): 25-30.
[9]	王星, 杨腾, 毛子昆, 蔺菲, 叶吉, 房帅, 戴冠华, 胡家瑞, 郝占庆, 王绪高, 原作强. 长白山阔叶红松林优势树种叶际真菌群落结构 [J]. 应用生态学报, 2022, 33(9): 2405-2412.
[10]	高慧芳, 孟婷, 熊琦, 章鸿宇, 邱君志, 林文雄, 张燎原. 太子参不同休耕年限土壤理化特征和微生物群落变化 [J]. 应用生态学报, 2022, 33(8): 2196-2204.
[11]	史宇骁, 李阳, 孟翊, 赵志远, 张婷玉, 王栋, 袁琳. 1989—2020年长江口九段沙湿地格局演变及影响因素 [J]. 应用生态学报, 2022, 33(8): 2229-2236.
[12]	杨贵森, 张志山, 赵洋, 石亚飞, 虎瑞. 沙坡头地区凋落物分解及其对土壤微生物群落的影响 [J]. 应用生态学报, 2022, 33(7): 1810-1818.
[13]	窦永静, 王让虎, 吴东辉. 冻融作用对大兴安岭多年冻土区土壤节肢动物的影响 [J]. 应用生态学报, 2022, 33(5): 1405-1412.
[14]	高贝, 胡艳宇, 张志委, 丁聪, 杨雁茹, 吕晓涛. 氮素添加对呼伦贝尔草甸草原植物氮钾元素含量和计量比的影响 [J]. 应用生态学报, 2022, 33(4): 981-987.
[15]	厉彦哲, 孙志高, 毛立, 陈冰冰, 胡星云, 王晓颖, 师自香. 闽江河口互花米草不同入侵年限湿地土壤磷赋存形态 [J]. 应用生态学报, 2022, 33(4): 1003-1011.