有机肥替代部分化肥对芒果丛枝菌根真菌群落的影响

doi:10.13287/j.1001-9332.202302.011

应用生态学报 ›› 2023, Vol. 34 ›› Issue (2): 481-490.doi: 10.13287/j.1001-9332.202302.011

有机肥替代部分化肥对芒果丛枝菌根真菌群落的影响

江尚焘¹, 栗晗¹, 彭海英¹, 梅新兰¹, 陈廷速², 徐阳春¹, 董彩霞^1*, 沈其荣¹

¹南京农业大学江苏省固体有机废弃物资源化高技术研究重点实验室/江苏省有机固体废弃物协同创新中心/教育部资源节约型肥料工程技术研究中心/资源与环境科学学院, 南京 210095;
²广西农业科学院微生物研究所, 南宁 530007

收稿日期:2022-03-04 接受日期:2022-11-15 出版日期:2023-02-15 发布日期:2023-08-15
通讯作者: *E-mail: cxdong@njau.edu.cn
作者简介:江尚焘, 男, 1990年生, 博士研究生。主要从事果树丛枝菌根真菌研究。E-mail: jshangtao@qq.com
基金资助:
国家重点研发计划项目(2017YFD0202101)和广西科技基地和人才专项(桂科AD20159001)

Effects of partial substitution of chemical fertilizer with organic fertilizer on arbuscular mycorrhizal fungal community of Mangifera indica

JIANG Shangtao¹, LI Han¹, PENG Haiying¹, MEI Xinlan¹, CHEN Tingsu², XU Yangchun¹, DONG Caixia^1*, SHEN Qirong¹

¹Jiangsu Provincial Key Laboratory for Organic Solid Waste Utilization/Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization/Engineering Center of Resource-Saving Fertilizers, Ministry of Education/College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;
²Institute of Microbiology Research, Guangxi Academy of Agricultural Sciences, Nanning 530007, China

Received:2022-03-04 Accepted:2022-11-15 Online:2023-02-15 Published:2023-08-15

摘要/Abstract

摘要： 农业生产施肥造成的养分富集会降低丛枝菌根真菌(AMF)的多样性。为了探究有机肥替代部分化肥对AMF的影响,本研究开展两年的芒果田间试验,以单施化肥为对照,设置两种有机肥(普通商品有机肥和生物有机肥)与12%(低)和38%(高)有机肥替代化肥处理,采用高通量测序技术比较不同施肥措施对芒果根内和根际土壤AMF群落特征的影响。结果表明: 在总养分投入相当的情况下,有机肥替代部分化肥对芒果产量和品质有一定的改善作用。施用有机肥可以有效增加芒果AMF的物种丰富度,且AMF的多样性与部分果实品质指标呈显著正相关。与单施化肥相比,高有机肥替代比例能够显著改变芒果根内AMF群落,但对根际土壤AMF群落没有显著影响。生物有机肥处理较普通商品有机肥处理能够富集更多的AMF物种,并形成更复杂的AMF共发生网络。综上,高比例有机肥替代化肥能够在改善芒果产量和品质的同时维持AMF的物种多样性,并且有机肥对AMF群落的改变优先在根内发生。

关键词: 丛枝菌根真菌, 有机肥, 化肥, 芒果, 高通量测序

Abstract: Nutrient enrichment caused by fertilization would reduce the diversity of arbuscular mycorrhizal fungi (AMF). To explore whether partial substitution of chemical fertilizer with organic fertilizer would alleviate the negative effects of nutrient enrichment on AMF, we conducted a two-year mango (Mangifera indica) field experiment to examine the effects of different fertilization regimes on AMF communities in roots and rhizospheric soils by using high-throughput sequencing. The treatments included chemical-only fertilization (control), and two kinds of organic fertilizer (commercial organic fertilizer and bio-organic fertilizer) with replacing 12% (low) and 38% (high) chemical fertilizer. The results showed that under equivalent nutrient input, partial substitution of chemical fertilizer with organic fertilizer had positive effects on the yield and quality of mango. The application of organic fertilizer could effectively increase AMF richness. AMF diversity was significantly positively correlated with some indices of fruit quality. Compared with chemical-only fertilization, high replacement ratio of organic fertilizer could significantly change root AMF community, but did not affect AMF community in the rhizospheric soil. Bio-organic fertilizer could enrich more AMF species and form a more complex AMF co-occurrence network than commercial organic fertilizer. In all, replacing chemical fertilizer with a high proportion of organic fertilizer could improve the yield and quality of mango while maintain AMF richness. The changes of AMF community caused by organic fertilizer substitution pre-ferably occurred in roots rather than soils.

Key words: arbuscular mycorrhizal fungi, organic fertilizer, chemical fertilizer, mango, high-throughput sequencing

江尚焘, 栗晗, 彭海英, 梅新兰, 陈廷速, 徐阳春, 董彩霞, 沈其荣. 有机肥替代部分化肥对芒果丛枝菌根真菌群落的影响[J]. 应用生态学报, 2023, 34(2): 481-490.

JIANG Shangtao, LI Han, PENG Haiying, MEI Xinlan, CHEN Tingsu, XU Yangchun, DONG Caixia, SHEN Qirong. Effects of partial substitution of chemical fertilizer with organic fertilizer on arbuscular mycorrhizal fungal community of Mangifera indica[J]. Chinese Journal of Applied Ecology, 2023, 34(2): 481-490.

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有机肥替代部分化肥对芒果丛枝菌根真菌群落的影响

Effects of partial substitution of chemical fertilizer with organic fertilizer on arbuscular mycorrhizal fungal community of Mangifera indica

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