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应用生态学报 ›› 2002, Vol. ›› Issue (12): 1587-1591.

• 研究论文 • 上一篇    下一篇

水稻苗期磷高效基因型筛选研究

郭玉春, 林文雄, 石秋梅, 梁义元, 陈芳育, 何华勤, 梁康迳   

  1. 福建农林大学, 福州, 350002
  • 收稿日期:2002-08-12 修回日期:2002-10-28
  • 通讯作者: 林文雄
  • 基金资助:
    福建省科技厅百千万人才资助项目(99-Z-204)

Screening methodology for rice (Oryza sativa) genotypes with high phosphorus use efficiency at their seedling stage

GUO Yuchun, LIN Wenxiong, SHI Qiumei, LIANG Yiyuan, CHEN Fangyu, HE Huaqin, LIANG Kangjing   

  1. Fujian Agriculture and Forestry University, Fuzhou 350002
  • Received:2002-08-12 Revised:2002-10-28

摘要: 采用难溶性磷酸盐Ca3(PO4)2为唯一磷源,在pH值为5.5条件下产生相对高浓度低磷胁迫及以NaH2PO4为磷源配制P浓度为0.5mg·L-1的相对低浓度低磷胁迫的两个水培环境,分别对不同基因型水稻的磷效率进行评价.以相对分蘖干重(RTW)、相对总生物量(RPW)、相对分蘖数(RTN)、相对根系干重(RRW)、相对地上部干重(RSW)、相对叶龄(RLA)和相对株高(RPH)作为耐性指标进行相关分析.结果表明,供试材料的磷效率存在极显著差异,若以能产生分蘖的相对高浓度低磷胁迫进行筛选时,相对分蘖干重、相对地上部干重、相对总生物量可作为较好的筛选指标,其中相对分蘖干重不仅与其它指标间的相关性强,且品种间差异和变异系数均较大,能准确、灵敏地反映不同基因型间的耐低磷胁迫能力;若采用相对低浓度的低磷胁迫对不同基因型水稻进行耐低磷种质筛选时,筛选指标则不同,最好的单一筛选指标应是相对地上部干重或相对总生物量.

关键词: 水稻, 基因型, 低磷胁迫, 筛选指标, 凋落物分解, 生态过程, 土壤氮循环, 季节性雪被, 全球气候变化

Abstract: Under hydroponic culture,the responses of rice (Oryza sativa) genotypes to low Pstress were respectively evaluated by means of relatively-higher Pconcentration in a controlled system with insoluble phosphate source (Ca3(PO4)2) and of relatively-lower concentration with 0.5 mg P/Lin which NaH2PO4 was the soluble phosphate. Relative indices such as relative dry weight of tillers (RTW),total relative plant dry weight (RPW),relative number of tillers (RTN),relative dry weight of root (RRW),relative dry weight of shoot (RSW),relative leaf age (RLA) and relative plant height (RPH) were used to screen as the tolerant indices to Pdeficiency,and the correlations among screening criteria per se were analyzed. The results showed that there were significant differences of tolerant indices to Pdeficiency in the test rice genotypes. When the rice cultivars were exposed to low Pstress with the relatively-higher Pconcentration to be screened,the relative tiller dry weight (RTW),relative shoot dry weight (RSW) and total relative plant dry weight (RPW) could be used as the better screening criteria,especially RTWwas considered as a sensitive and reliable screening criterion because its coefficient of variation (CV) and the variable range of data among the test varieties should be large. However,when rice genotypes were grown in the stress condition with relatively-lower Pconcentration,the screening criteria were diffenent,and it suggested that relative shoot dry weight (RSW) or total relative plant dry weight (RPW) was the best single screening criterion.

Key words: Rice (Oryza sativa), Genotype, Low P stress, Screening criteria, global climate change, litter decomposition, seasonal snow-cover, soil N cycling, ecological process

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