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应用生态学报 ›› 2002, Vol. ›› Issue (9): 1153-1156.

• 研究论文 • 上一篇    下一篇

环境因子对日本沼虾消化酶和碱性磷酸酶的影响

王维娜1, 孙儒泳1, 王安利2, 鲍蕾2, 汪鹏2   

  1. 1. 北京师范大学生命科学学院, 北京 100875;
    2. 河北大学生命科学学院, 保定 071002
  • 收稿日期:2001-05-10 修回日期:2001-10-08 出版日期:2002-09-15
  • 通讯作者: 王维娜,女,1965年生,博士,教授,主要从事水生动物生理生态学研究,发表论文60余篇.Tel:0312-5079617,E-mail:Wangwn@mail.hbu.edu.cn
  • 基金资助:
    河北省重点科技攻关资助项目(96220502D)

Effect of different environmental factors on the activities of digestive enzymes and alkaline phosphatase of Macrobrochium nipponense

WANG Weina1, SUN Ruyong1, WANG Anli2, BAO Lei2, WANG Peng 2   

  1. 1. Department of Beiology, Beijing Normal University, Beijing 100875;
    2. Department of Beiology, Hebei University, Baoding 071002
  • Received:2001-05-10 Revised:2001-10-08 Online:2002-09-15

摘要: 研究了水环境中不同Ca2+浓度(20、35、60、80和150mg·L-1)、盐度(7、14和20‰)和pH(76、88和98)对日本沼虾(Macrobrochium nipponense)肝胰腺中消化酶(胃蛋白酶和类胰蛋白酶)和碱性磷酸酶的影响.结果表明,Ca2+对日本沼虾的胃蛋白酶有促进作用,Ca2+浓度为150mg·L-1时酶活力最高;高浓度的Ca2+对类胰蛋白酶有抑制作用,而在一定范围内,碱性磷酸酶活力随Ca2+浓度的增高而增高.盐度为14‰时,日本沼虾的胃蛋白酶、类胰蛋白酶和碱性磷酸酶活力均高于7‰和20‰组.随着pH值升高,蜕皮率和3种酶活力也随之增高,pH98时均达到最高值,但增长率和增重率则降低.

关键词: 日本沼虾, 消化酶, 碱性磷酸酶, 钙, 盐度, pH

Abstract: The activities of digestive enzymes and alkaline phosphatase from the hepatopancreas of Macrobrochium nipponense were determined under different environmental factors (calcium concentrations 20 mg·L-1,35 mg·L-1,60 mg·L-1,80 mg·L-1,150 mg·L-1 ;salinity 7‰,14‰,20‰;pH7 6,88,98).The results showed that higher Ca2+ concentration could enhance the pepsin activity,but inhibit the trysin-like activity in hepatopancreas of M. nipponense.The activities of pepsin,trysin-like,alkaline phosphatase in hepatopancreas of M. nipponense were higher under salinity of 14‰ than under salinity of 7‰ and 20‰.It showed that the activities of digestive enzymes and alkaline phosphatase of shrimp increased gradually with increasing pHvalue from 7.6 to 9.8.

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