硅酸盐细菌NBT菌株在小麦根际定殖的初步研究

应用生态学报 ›› 2003, Vol. ›› Issue (11): 1914-1916.

硅酸盐细菌NBT菌株在小麦根际定殖的初步研究

盛下放

南京农业大学生命科学学院, 南京 210095

收稿日期:2001-01-15 修回日期:2001-04-01
基金资助:
上海市农业委员会项目(农科攻字(98)第052号);江苏省教育厅社会发展资助项目(BS2002044)

Colonization of silicate bacterium strain NBT in wheat roots

Sheng Xiafang

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China

Received:2001-01-15 Revised:2001-04-01

摘要/Abstract

摘要： 对硅酸盐细菌NBT菌株进行了耐药性标记,得到稳定的链霉素抗性标记菌NBT菌株.采用选择性培养基分离计数,通过琼脂平板和盆栽试验,研究了标记菌NBT在小麦根际的定殖动态及影响因素.结果表明,在灭菌土盆栽中,播种后9d左右NBT菌株在小麦根际的定殖水平达最高(1.4×10⁸cfu·g^-1根土),播种后54d左右趋向稳定,NBT菌株细胞数量为2.4×10³cfu·g^-1根土;未灭菌土盆栽中,播种后9d左右NBT菌株的定殖水平达最高(3.8×10⁸cfu·g^-1根土),60d左右趋向稳定,菌数为3.1×10³cfu·g^-1根土.生物和非生物因素对NBT菌株定殖小麦根系有影响.

关键词: 耐药菌株, 小麦根际, 定殖动态

Abstract: The strain NBT of silicate bacterium was labelled with streptomycin,and a stable streptomycin resistance strain NBT was obtained.Its colonization dynamics and affecting factors in wheat rhizosphere were studied in agar plates and greenhouse pots were studied by counting the method with selective medium. The results of pot culture experiment showed that strain NBT could successfully colonize in the rhizosphere of wheat.In pot cultures of sterile soil,the highest colonization level (3.4?10⁷cfu穏^-1 root soil) was reached on9th day after seeds sown;at 54th day, the population of strain NBT tended to stable, and decreased to 1.4?10⁴cfu穏^-1 root soil.In pot cultures of unsterile soil, the highest colonization level (3.8?10⁷cfu穏^-1 root soil) was reached at 9th day,and the population of strain NBT tended to a stationary state at 60th day,with the numbers being 1.4?10⁴cfu穏^-1 root soil. Some biological and abiotic factors could greatly influence the colonization of the beneficial microorganism.

Key words: Streptomycin resistance strain, Wheat rhizosphere, Colonizing dynamics

中图分类号:

Q938.1

盛下放. 硅酸盐细菌NBT菌株在小麦根际定殖的初步研究[J]. 应用生态学报, 2003, (11): 1914-1916.

Sheng Xiafang. Colonization of silicate bacterium strain NBT in wheat roots[J]. Chinese Journal of Applied Ecology, 2003, (11): 1914-1916.

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