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应用生态学报 ›› 2003, Vol. ›› Issue (3): 405-408.

• 研究论文 • 上一篇    下一篇

利用定量杂交方法对几个人工和环境微生物样品的分析研究

黄立南, 陈月琴, 周惠, 屈良鹄   

  1. 中山大学生命科学学院, 基因工程教育部重点实验室, 广州 510275
  • 收稿日期:2001-02-07 修回日期:2001-04-20
  • 通讯作者: 屈良鹄
  • 基金资助:
    国家杰出青年基金项目(39525007);国家自然科学基金项目(39970063);广东省自然科学基金资助项目(011120)

Characterization of several synthetic and environmental microbial samples by quantitative hybridization

HUANG Linan, CHEN Yueqin, ZHOU Hui, QU Lianghu   

  1. College of Life Sciences, Key Laboratory of Gene Engineering, Ministry of Education, Zhongshan University, Guangzhou 510275, China
  • Received:2001-02-07 Revised:2001-04-20

摘要: 利用真细菌和真核生物两个域特异性探针,初步建立起16SrRNA定量杂交的方法,并对几个人工RNA样品(由提取自S.cerevisiaeP.fluorescens纯培养细胞的RNA按一定比例混和而成)和一个提取自垃圾填埋场渗滤液的RNA样品进行了初步的定量分析.结果表明该方法能从域的水平上对这些样品的组成作较精确的分析,从而在微生物生态学领域具有广泛的应用前景.

关键词: 16SrRNA, 寡核苷酸探针, 定量杂交, 微生物生态, 火烧强度, MTCLIM模型, 3PGS模型, 地形因子, 净初级生产力

Abstract: Limitations of traditional techniques based on selective enrichment and pure culture isolation make it difficult to precisely characterize the natural microbial ecosystems. Molecular techniques are now being developed and used to address these limitations. Group specific 16SrRNAs targeted oligonucleotide probes of different phylogenetic levels are increasingly used to identify and quantify the microbial members in complex environmental samples. Two domain specific probes were used in this study to elementarily characterize the defined mixtures of RNAs extracted from pure culture (synthetic samples) and a RNAsample obtained from landfill leachate (environmental sample). The results demonstrated that 16SrRNAquantitative hybridization provided an excellent estimation of domain level community composition of these samples, and thus, had a huge potential of usefulness in microbial ecology studies.

Key words: 16S rRNA, Oligonucleotide probes, Quantitative hybridization, Microbial ecology, MTCLIM model, net primary productivity, topographic factor., 3PGS model, fire severity

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