长期培肥黑土微生物量磷动态变化及影响因素

摘要/Abstract

摘要： 长期采用两种不同量有机肥(M₂、M₄)、化肥(NPK)方式培肥黑土,研究微生物量P在作物生长季动态变化.结果表明,施用有机肥微生物量P显著高于施用化肥(NPK)和不施肥(CK),微生物量P分别为M₄ 8.75～47.68 mg·kg^-1,M₂ 3.02～37.16 mg·kg^-1,NPK 1.59～10.62 mg·kg^-1,CK 0.76～6.74 mg·kg^-1之间,波动性较大.M₄、M₂处理微生物量P最大值出现在抽雄吐丝期,NPK、CK处理最大值出现在大喇叭口期;施肥数量和种类不同所引起的黑土微生物量P的差异并未因季节变化及玉米生育时期影响而明显改变.微生物量P的动态变化与绝大多数黑土生物、理化特性指标的动态变化没有显著的相关性;微生物量P与黑土生物、理化特性(除全钾外),植物氮、磷、钾含量有极显著的正相关关系,与黑土含水量呈显著正相关关系.

关键词: 黑土, 有机肥, 微生物量磷, 生长季, 动态变化

Abstract: This paper studied the microbial biomass P in a long-term fertilized black soil.The soil was fertilized by farmyard manure(M₂,M₄)and chemical fertilizer(NPK),and the dynamics of soil microbial biomass P was monitored during crop growth season.The results showed that the microbial biomass P in different treatments was 8.75～47.68 mg·kg^-1(M₄),3.02～37.16 mg·kg^-1(M₂),1.59～10.62 mg·kg^-1(NPK),and 0.76～6.74 mg·kg^-1(CK).The microbial biomass P in M₄ and M₂ was the highest at reproductive growth stage,and that in NPK and CK was the highest at early growth stage.The significant difference of soil microbial biomass P induced by different amounts and types of fertilizer application was not varied with the period and stage of plant growth.In all treatments,no significant correlation was found between the dynamics of soil microbial biomass P and the soil biological,physical and chemical properties in plant growth period.Soil microbial biomass P had a very significant positive correlation with soil biological,physical and chemical properties(expect K),and a significant positive correlation with the N,P and K contents of plants and soil water content.

Key words: Black soil, Farmyard manure, Microbial biomass P, Growth season, Dynamics

中图分类号:

S154.2

李东坡, 武志杰, 陈利军, 朱平, 任军, 梁成华, 彭畅, 高红军. 长期培肥黑土微生物量磷动态变化及影响因素[J]. 应用生态学报, 2004, (10): 1897-1902.

LI Dongpo, WU Zhijie, CHEN Lijun, ZHU Ping, REN Jun, LIANG Chenghua, PENG Chang, GAO Hongjun. Dynamics of microbial biomass P and its affecting factors in a long-term fertilized black soil[J]. Chinese Journal of Applied Ecology, 2004, (10): 1897-1902.

参考文献

[1] Chen G-C(陈国潮).1999.Methods for measuring soil microbial biomass now and its aplicationin red soil.Chin J Soil Sci(土壤通报),30(6):284～287(in Chinese)
[2] Chen G-C(陈国潮).2001.Study on transformation and utilization of microorganisnsforfixation phosphate in soil. Chin J Soil Sci(土壤通报),32(2):80～82(in Chinese)
[3] Chen GC,He ZL.2003.Effect of land on microbial biomass-C,-N and-Pin red soil.J Zhejiang Univ(SCIENCE),4(4):480～484 (in Chinese)
[4] Chen G-C(陈国潮),He Z-L(何振立),Huang C-Y(黄昌勇).1999.Study on relationship between microbial biomass phosphorus and soil phosphorus in red soils.J Zhejiang Univ(Agric & Life Sci)(浙江大学学报·农业与生命科学版),25(5):513～516(in Chinese)
[5] ChenG-C(陈国潮),He Z-L(何振立),Huang C-Y(黄昌勇).2001. Study on relationships among microbial biomass P, soil P and plant-availability of P in red soils. Acta Pedol Sin (土壤学报), 38 ( 1 ) : 76 ～ 80( in Chinese)
[6] Frank WS. 2002. The phosphate uptake mechanism. Plant Soil,245:105～ 114
[7] Gyaneshwar P, Naresh KG, Parekh LJ. 1998. Effect of buffering on the phosphate-solubilizing ability of microorganisms. World J Microbiol, 14: 669 ～ 673
[8] Gyaneshwar P, Naresh KG, Parekh LJ, et al. 2002. Role o soil microoganisms in improving P nutrition of plants. Plant Soil, 245: 83～93
[9] He Z-L(何振立). 1997. Soil microbial biomass and its important role in nutrient cycles and assessing for environment qualities. Soil (土壤), 2:61～69(in Chinese)
[10] He ZL, Wu J, Odonne AG, et al. 1997. Seasonal responses in microbial biomass carbon, phosphorus and sulphur in soils under pasture. Biol Fert Soil, 24:421 ～428
[11] He ZL, Zhu J. 1998. Microbial utilization and transformation of P sorbed by variable-charge minerals. Soil Biol Biochem, 30:917～923
[12] Huang M(黄敏), Wu J-S(吴金水), Huang Q-Y(黄巧云).2003. Process in research on microbiological action of soil phosphorus. Ecol Environ(生态环境), 12(3) :366～ 370(in Chinese)
[13] Kenji Kouno, Yasuhiro Tuchiya and Tadao Ando. 1995. Measurement of soil microbial biomass phosphorus by an anion exchange membrane method. Soil Biol Biochem, 27(10): 1353～ 1357
[14] Kenji Kouno, Wu J, Brookes PC. 2002. Turnover of biomass C and P in soil following incorporation of glucose or ryegrass. Soil Biol Biochem, 34: 617 ～ 622
[15] Li D-p(李东坡), Wu Z-J(武志赤), Chen L-J(陈利军). 2003. Dynamics of urease activity in a long-term fertilized black soil and its affecting factors. Chin J Appl Ecol ( 应用生态学报 ), 14 (12): 2208～2212(in Chinese)
[16] Li Y-K(李酉开). 1983. Conventional Analysis Methods of Soil Agricultural Chemistry. Beijing: Science Press. 60 ～ 285 ( in Chinese)
[17] LiangW(梁巍),YueJ(岳进),Wu J(吴劼 ), et al. 2003.Seasonal variation of soil, microbial biomass respiration rate and CH₄ emission in black earth rice fields. Chin J Appl Ecol (应用生态学报 ), 14 (12) : 2278 ～ 2280 (in Chinese)
[18] Lu R-K(鲁如坤). 1999. Analysis Methods of Soil Agricultural Chemistry. Beijing: China Agricultural Scicnce and Technology Press. 105 ～ 254 (in Chinese)
[19] Lu W-L(陆文龙), Zhang F-S(张福锁), Cso Y-P(曹一平). 1998.Advance in phosphorus behavior in soil. Tianjin Agric Sci (天津农业科学), 4(4): 1 ～7(in Chinese)
[20] Luo Ancheng, Sun Xi, Zhang Yongsong. 1993. Species of inorganic phosphate solubilizing bacteria in red soil and the mechanism of solubilization. Pedosphere, 3: 285 ～ 288
[21] Luo Ancheng, Sun Xi. 1994. Effect of organic manure on the biological activities associated with insoluble phosphorus release in a blue purple paddy soil. Commun Soil Sci Plant Anal, 13 ～ 14:2513～2522
[22] Oberson A, Friesen DK, Morel C, et al. 1997. Determination of phosphorus released by chloroform fumigation from microbial biomass in high P sorbing tropical soils. Soil Biol Biochem, 29:1579～ 1583
[23] Ocio JA, Brookes PC. 1990. An evaluation of methods for measuring the microbial biomass in soils following recent additions of wheat straw and the characterization of the biomass that develops.Soil Biol Biochem, 22: 685 ～ 694
[24] Rao Z-H(饶正华),Lin Q-M(林启美),Sun Y-X(孙焱鑫), et al.2002. Interactions between a Bacillus mucilaginosus, phosphobacteria and a nitrogen fixing bacterium. Chin J Ecol( 生态学杂志 ),21(2) :71 ～73(in Chinese)
[25] Smith JL, Paul EA, Bollag P, et al. 1991. The significance of soil microbial biomass estimations. Soil Biochem, 6: 359～396
[26] Song Q-H(宋秋华), Li F-M(李凤民), Liu H-S(刘洪升), et al.2003. Effcet of film mulching on soil microbial biomass in spring wheat field in semi-arid loess area. Chin J Appl Ecol ( 应用生态学报), 14(9): 1512～ 1516(in Chinese)
[27] Sun Y-X(孙焱鑫), Lin Q-M(林启美), Zhao X-R(赵小蓉). 2003.Interaction of protozoa and phosphate-solubilizing bacteria on rock phosphate dissolution. Chin J Ecol(生态学杂志), 22(3): 84 ～ 86 (in Chinese)
[28] Tessier L, Gregorich EG, Topp E. 1998. Spatial variability of soil microbial biomass measured by the fumigation extraction method and KEC as affected by depth and manure application. Soil Biol Biochem, 30:1369 ～ 1377
[29] Wick B, Kuhne RF, Vlek PLG. 1998. Soil microbiological parameters as indicators of soil quailty under improved fallow management systems in southwestern Nigeria. Plant Soil, 202:97～ 107
[30] Wu J-S(吴金水), Xiao H-A( 肖和艾), Chen G-Q(陈桂秋).2003.Measurement of microbial biomass-P in upland soil in China. Acta Pedol Sin ( 土壤学报 ), 40 ( 1 ): 77 ～ 78 (in Chinese)
[31] Yaneshwar G, Naresh Kumar PG, Parekh LJ, et al. 2002. Role of soil microorganisms in improving P nutrition of plants. Plant Soil,245: 83 ～ 93
[32] Youssef RA, Chino M. 1989. Root induced changes in the rhizosphere of plants Ⅱ. Distribution of heavy metals across the rhizosphere in soils. Soil Sci Plant Nutr, 4: 609 ～ 621
[33] Zhang C-E(张成娥), Wang S-Q(王栓全), et al. 2000. Study on soil microbial biomass during decomposition of crop straws. J Soil Water Conser (水土保持学报), 14( 3 ): 96 ～ 99( in Chinese)
[34] ZhangJ-E(章家恩),Liu W-G(刘文高), Hu G(胡刚).2002.The relationship between quantity index of soil microorganisms and soil fertility of different land use systems. Soil Environ Sci (土壤与环境), 11 (2): 140～ 143(in Chinese)