~(32)P示踪法研究溶磷真菌对磷肥转化固定和有效性的影响

应用生态学报 ›› 2004, Vol. ›› Issue (11): 2142-2146.

~(32)P示踪法研究溶磷真菌对磷肥转化固定和有效性的影响

范丙全, 金继运, 葛诚

中国农业科学院土壤肥料研究所, 北京 100081

收稿日期:2002-10-12 修回日期:2003-02-28
通讯作者: 范丙全,1956生,男,博士,研究员,主要从事土壤微生物与农业微生物资源利用研究,发表论文30余篇.Tel:010-68918683;Fax:010-68918636;E-mail:Bqfan@caas.ac.cn
基金资助:
国家自然科学基金资助项目(30270048)

Effects of phosphate-dissolving fungi on transformation,fixation and efficiency of fertilizer ³²P

FAN Bingquan, JIN Jiyun, GE Cheng

Soil and Fertilizer Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2002-10-12 Revised:2003-02-28

摘要/Abstract

摘要： 采用³²P示踪技术,研究了溶磷青霉菌P8对肥料磷与土壤有效磷的转化、固定和有效性的影响.结果表明,溶磷青霉菌菌剂能够增加玉米、花生的生物量,促进作物对土壤和肥料磷素的吸收;溶磷菌剂具有防止有效磷转化为难溶Ca₁₀-P的作用,增加有效态磷(Ca₂-³²P、Ca₈-³²P)的比例.随时间延长,施入的³²P转化为Ca₁₀-P的数量(或比例)逐渐增加,但是相对于未接种菌剂处理,接种青霉菌菌剂的土壤磷和肥料磷转化为Ca₁₀-P比例最低.溶磷青霉菌菌剂不仅能够防止有效磷向难溶磷Ca₁₀-P的转化,而且其效果能够维持较长时间.

关键词: ³²P示踪技术, 溶磷真菌, 磷肥, 有效性

Abstract: By using a radioactive ³²P-labeled technique,this paper studied the effects of P-dissolving Penicillium oxalicum P8 inoculum on the transformation,fixation and efficiency of fertilizer P.The results showed that the inoculum could prevent available P from fixation by soil,and promoted the uptake of applied P by maize and peanut.Additionally,it reduced the amount of Ca₁₀ -P fraction derived from available P,increased the pool of available P,and maintained more ³²P in forms of Ca₂-P and Ca₈-P.With time extending,the ratio of Ca₁₀ -P fraction in soil was gradually increased,and the treatment with P.oxalicum P8 showed the lowest ratio of Ca₁₀-P form,compared with treatment without P.oxalicum P8.The inoculum not only prevented available ³²P from turning into Ca₁₀ -P form,but also had a longer effective period.

Key words: ³²P isotopic technique, P-dissolving fungi, Phosphate fertilizer, Availability

中图分类号:

S143.2

范丙全, 金继运, 葛诚. ~(32)P示踪法研究溶磷真菌对磷肥转化固定和有效性的影响[J]. 应用生态学报, 2004, (11): 2142-2146.

FAN Bingquan, JIN Jiyun, GE Cheng. Effects of phosphate-dissolving fungi on transformation,fixation and efficiency of fertilizer ³²P[J]. Chinese Journal of Applied Ecology, 2004, (11): 2142-2146.

参考文献

[1] Arora DI,Gaur AC.1978.Periodic microbial solubilization of ³²P labeled hydroxyapatite.Ind J Exp Biol,18(3):193～194
[2] Azcon-Aguilar C,et al.1986.Effect of vesicular-arbuscular mycorrhizal fungi and phosphate- solubilizing bacteria on growth and nutrition of soybean in a neutral-calcareous soil amended with ³²P-45Ca-tricalcium phosphate.Plant Soil,96(1):3～15
[3] Barea JM,Toro M,Orozco MO,et al.2002.The application of isotopic (³²P and 15N) dilution techniques to evaluate the interactive effect of phosphate-solubilizing rhizobacteria,mycorrhizal fungi and rhizobium to improve the agronomic efficiency of rock phosphate for legume crops.Nutr Cyc Agroecosyst,63(1):35～42
[4] Ford MC.1933.The nature of phosphate fixation in soils.J Am Soc Agron,25:133～144
[5] Gu Y-C(顾益初),Jiang B-F(蒋柏藩).1989.Study on fractioning systems of inorganic phosphate in calcareous soils.Sci Agric Sin(中国农业科学),22(3):58～66(in Chinese)
[6] Gu Y-C(顾益初),Jiang B-F(蒋柏藩).1990.Determination method of the fractionation of inorganic phosphorus in calcareous Chao soils.J Soil Sci(土壤),22(2):101～102(in Chinase)
[7] Gu Y-C(顾益初),Li A-R(李阿荣),Jiang B-F(蒋柏藩).1991.Phosphorous supplying capacity of different phosphate sources in calcareous Chao soils.J Soil Sci(土壤),23(6):296～301(in Chinase)
[8] Jiang B-F(蒋柏藩).1992.Study on availability of soil inorganic phosphate in calcareous soil.J Soil Sci(土壤),24(2):61～64(in Chinese)
[9] Jin J-Y(金继运).1995.A systematic approach for soil nutrient status study and its application.Acta Pedol Sin(土壤学报),32(1):84～90 (in Chinese)
[10] Murrmann RP,Peech M.1968.Reaction products of applied phosphate in limed soils.Soil Sci Soc Am Proc,32:493～496
[11] Nagelschmidt G,Nixon HL.1944.Formation of apatite from superphosphate in the soil.Nature,154:428～429
[12] Raj J,Bagyaraj DJ,Manjunath A.1981.Influence of soil inoculation with vesicular-arbuscular mycorrhiza and a phosphate dissolving bacterium on plant growth and ³²P uptake.Soil Biol Biochem,13(2):105～108
[13] Zheng C-R(郑春荣),Chen H-M(陈怀满),Zhou D-M(周东美),et al.2002.Chemical depletion of cumulative phosphorus in soils.Chin J Appl Ecol(应用生态学报),13(5):559～563 (in Chinese)

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