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应用生态学报 ›› 2004, Vol. ›› Issue (12): 2233-2236.

• 研究论文 • 上一篇    下一篇

6-苄(基)腺嘌呤和抗坏血酸对渗透胁迫时杨树光合作用光抑制的影响

冯玉龙1,2, 马永双2, 冯志立1   

  1. 1. 中国科学院西双版纳热带植物园昆明分部, 昆明 650223;
    2. 河北大学生命科学学院, 保定 071002
  • 收稿日期:2003-04-16 修回日期:2004-07-02
  • 通讯作者: 冯玉龙,男,1964年生,博士.研究员.主要从事植物生理生态学、入侵生态学研究.发表论文70多篇.Tel:0871-5163626;E-mail:fyl@xtbg.ac.cn.
  • 基金资助:
    国家“九五”科技攻关资助项目(960110102)

Effects of 6-BA and AsA on photosynthesis photoinhibition of attached poplar leaves under osmotic stress of root

FENG Yulong1,2, MA Yongshuang2, FENG Zhili 1   

  1. 1. Kunming Division, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China;
    2. College of Life Science, Hebei University, Baoding 071002, China
  • Received:2003-04-16 Revised:2004-07-02

摘要: 研究了6-BA和AsA对渗透胁迫时杨树幼苗叶片光合作用光抑制和活性氧代谢的影响.结果表明,渗透胁迫时杨树叶片净光合速率(Pn)和表观量子效率(AQY)降低,光合作用光抑制加剧,超氧化物歧化酶(SOD)活性升高,抗坏血酸过氧化物酶(APX)活性降低,O2-·产生加快,H2O2和膜脂过氧化产物丙二醛(MDA)含量升高.6-BA和AsA预处理使胁迫时叶片SOD和APX活性升高,O2-·生成减少,H2O2和MDA含量降低,同时缓解了光合作用的光抑制.相关分析表明,杨树叶片活性氧水平和MDA含量与Pn和AQY呈负相关.胁迫时杨树叶片活性氧的积累与光合作用光抑制有一定关系,6-BA和AsA对光抑制的缓解作用与其对活性氧清除系统的促进作用有关.

关键词: 杨树无性系, 渗透胁迫, 光合作用光抑制, 活性氧, 保护酶, 6-BA, AsA

Abstract: In order to know more about the relationships between photosynthesis photoinhibition and reactive oxygen species metabolism,the effects of 6-benzyladenine (6-BA) and ascorbate (AsA) on net photosynthetic rate (Pn),apparent quantum yield (AQY),superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities,O2-? generation rate,and H2O2 and malondialdehyde (MDA) contents were studied with attached leaves of poplar clone seedlings under osmotic stress of root.Under osmotic stress,the photosynthesis photoinhibition of attached poplar leaves,judged by the significant decrease of Pn and AQY,was aggravated,and the balance of reactive oxygen species metabolism was destroyed.The superoxide dismutase (SOD) activity increased,but ascorbate peroxidase (APX) activity decreased.In the meantime,the O2-? generation rate and the contents of H2O2 and malondialdehyde (MDA) increased.When osmotic stressed poplar seedlings were pretreated with 6-BA and AsA,the activities of SOD and APX increased,O2-? generation rate and H2O2 and MDA contents decreased,and photosynthesis photoinhibition was alleviated.The contents of reactive oxygen species and MDA in poplar leaves were negatively correlated with net photosynthetic rate and apparent quantum yield.It's indicated that the photosynthesis photoinhibition of attached leaves of poplar clone seedlings had intrinsic relations with the accumulation of reactive oxygen species under osmotic stress of root,and the alleviation effects of 6-BA and AsA on photosynthesis photoinhibition were related to their promotion effects to the scavenging system of reactive oxygen species.

Key words: Poplar clone, Osmotic stress, Photosynthesis photoinhibition, Reactive oxygen species, Protecting enzymes, 6-BA, AsA

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