球孢白僵菌种群在松林中的寄主转移及遗传多样性对松毛虫持续控制的影响

摘要/Abstract

摘要： 通过林间接种式放菌及其后一周年的野外调查,从林间采集到30种寄主昆虫及从土壤、落叶和气流中分离到119株球孢白僵菌.酯酶同工酶分析表明,它们属于32个不同酯酶型,呈现出丰富的遗传多样性.释放菌株所属的酯酶型02包括从11种昆虫上分离出的18个菌株,表明林间释放的菌株已成功地在不同寄主昆虫种群中宿存下来,并以常发的地方病状态存在于松毛虫及松灰象甲等12种昆虫种群之中;当林间目标寄主缺乏时,其它寄主可将食物链维系下去.其它酯酶型分别包括1～23个菌株.一周年内的寄主转移动态结果表明,球孢白僵菌在松林生态系统中不同寄主间可转移寄生.每个酯酶型中的菌株对松毛虫的毒力相差很大.表明球孢白僵菌在松林中的延续和扩散流行不是1条路线,每个酯酶型至少代表食物网上的1条支链.有些环节的寄主连接了不同的酯酶型,使松林中食物网变得十分复杂.另外,从土壤、枯枝落叶层、林冠层和空气中分离到的球孢白僵菌分属于不同的酯酶型,表明松林中还存在着复杂的腐生食物链,有利于松毛虫及其它害虫的持续控制.

关键词: 球孢白僵菌, 寄主转移, 遗传多样性, 持续控制, 酯酶同工酶

Abstract: In an anniversary investigation post inoculative release of Beauveria bassiana against Masson's pine caterpillars in a Masson's pine plantation,119 strains were isolated from 30 host insects,soil,litter and air.Based on an esterase isozyme analysis,all these strains were assigned to 32 esterase types,suggesting abundant genetic diversity.The esterase type 2 was assigned including 18 other strains from 11 different host insects,indicating that the released strain had successfully survived in different insect populations and persisted in enzootic condition in 12 insect populations including pine caterpillars and grey pine weevils.Other host insects could maintain the food chain when the target insect population was low.The other esterase types included 1～23 strains.The anniversary host transfer dynamics further revealed that B.bassiana could transfer among different host insects in the pine ecosystem.The virulence of the strains of each esterase differed substantially,which suggested that B.bassiana persisted and dispersed along more than one route,and each esterase stood for at least one branch in the food web.Some hosts connected different esterase types at nodal points,making the food web very complicated.In addition,the strains isolated from soil,litter,forest crown and air belonged to different esterase types,suggesting that a complex of saprophytic food chains existed in the plantation,which made the food web more complicated,and benefited the sustainable control of the Masson's pine caterpillar and other pest insects in the ecosystem.

Key words: Beauveria bassiana, Host transfer, Genetic diversity, Sustainable pest control, Esterase isozyme

中图分类号:

Q968.1

丁德贵, 李增智, 樊美珍, 王滨. 球孢白僵菌种群在松林中的寄主转移及遗传多样性对松毛虫持续控制的影响[J]. 应用生态学报, 2004, (12): 2315-2320.

DING Degui, LI Zengzhi, FAN Meizhen, WANG Bin . Host transfer of Beauveria bassiana population in pine stand ecosystem and impact of its genetic diversity on sustainable control of Masson's pine caterpillars[J]. Chinese Journal of Applied Ecology, 2004, (12): 2315-2320.

参考文献

[1] Ferron P. 1997. Fungal control in comprehensive insect physiology.In:Kerkut GA, Gilbert LI, eds. Biochemistry and Pharmacology.Great Britain: Pergamon Press. 313～346
[2] Fuxa JR. 1987. Ecological consideration for the use of entomopathogens. Ann Rev Ent, 32: 225～251
[3] Gottwald TR, Tedders WL. 1984. Suppression of pecan weevil populations with entomopathogenic fungi. Environ Ent, 12:471～474
[4] Han B-Y(韩宝瑜), Li Z-Z(李增智), Chen G-P(陈国平), et al.1996. Study on pathogenecity of Beauveria bassiana on non-target invertebrates. J Anh ui Agric Univ ( 安徽农业大学学报 ), 23 ( 3 ):404～410(in Chinese)
[5] Han B-Y(韩宝瑜), Li Z-Z(李增智). 1997. Numeral, temporal and spatial patterns of animal and entomogenous fungus communities in Masson pine stands with reasonable chemical control. Chin J Appl Ecol(应用生态学报), 8(1) :65～69(in Chinese)
[6] Hegedus DD, Khachatourians GG. 1996. Detection of the entomopathogenic fungus Beauveria bassiana within infected migratory grasshoppers(Melanoplus sanguinipes ) using polymerase chain reaction and DNA probe. J Inverteb Pathol, 30:191～200
[7] Horton DR. 1993. Behavioral effects of parasites and pathogens in insect hosts. In: Parasites and Pathogens of Insects. New York: Academic Press Inc. 107～124
[8] Huang B(黄勃), Li Z-Z(李增智), Fan M-Z(樊美珍), et al.2002. Molecular identification for the teleomorph of Beauveria bassiana. Mycotaxon (真菌分类学报), 81: 229～236(in Chinese)
[9] Ignoffo CM. 1985. Manipulating enzootic-epizootic diseases of arthropods. In:Hoy MA, Herzog DC, eds. Biological Control in Agricultural IPM Systems. Orlando: Academic Press Inc. 243～262
[10] Li Z-Z (李增智 ). 1988. A list on insect hosts of Beauveria bassiana. In:Study and Application of Entomogenous Fungi in China. Beijing:Academic Periodical Press. 241～255(in Chinese)
[11] Li Z-Z(李增智). 1993. Microbial control of forest pests. In: Study and Application of Entomogenous Fungi in China. Vol. 3. Beijing:Academic Periodical Press. 1～7(in Chinese)
[12] Li Z-Z(李增智), Hah B-Y(韩宝瑜), Fan M-Z(樊美珍). 1998.Strategies of applying Beaveria bassiana against masson pine caterpillar and their biodiversity basis. Chin J Appl Ecol ( 应用生态学报), 9(5) :503～510(in Chinese)
[13] Li Z-Z(李增智), Huang B(黄勃), Fan M-Z(樊美珍), et al.1998. Detection of variation between 3 species of Beauveria and isolates of Beauveria bassiana by RAPD-PCR techniques. Mycosystem (菌物系统), 17(2): 185～189(in Chinese)
[14] Lin H-F(林华峰), Li Z-Z(李增智), Fan M-Z(樊美珍). 1996.The study of Beauveria bassiana strain characteristics and different strain types in forest in Xuanzhou County. J Anhui Agric Sci (安徽农业科学), 24(supp. ) :49～52(in Chinese)
[15] Lin H-F(林华峰), Fan M-Z(樊美珍), Li Z-Z(李增智). 1998.Pathogenic effect of Beauveria bassiana infected on Dendrolimus punctatus under different temperature and humidity. Chin J Appl Ecol (应用生态学报), 9(2): 195～200(in Chinese)
[16] Lin H-F(林华峰), Li Z-Z(李增智), Hu C(胡萃). 1999. Influence of drug form and host state on germination and infection of Beauveria bassiana. Chin J Appl Ecol (应用生态学报), 10(5):612～614(in Chinese)
[17] Liu Y-Z(刘玉珍), Han B-Y(韩宝瑜). 1995. Structure and timespatial patterns of community of entomogenous fungi and animals in masson pine plantation stands with Beauveria bassiana as regular inoculum agent. J Biomathem (生物数学学报), 10 (3): 205～216(in Chinese)
[18] Studdert JP, Kaya HK. 1990a. Water potential, temperature, and soil type on the formation of Beauveria bassiana soil colonies. J Invertebr Pathol, 56: 380～386
[19] Studdert JP, Kaya HK. 1990b. Water potential, temperature, and clay-coating on survival of Beauveria bassiana conidia: Effect on Spodoptera exigua pupal mortality in two soil types. J Invertebr Pathol, 56: 327～336
[20] Tang J (汤坚). 1991. Study on esterase isozyme of 47 strains of Beauveria bassiana. In: Study and Application of Entomogenous Fungi of China. Vol. 2. Beijing: Academic Periodical Press. 104～108(in Chinese)
[21] Wang B(王滨),Fan M-Z(樊美珍),Li Z-Z(李增智).2000.Sieving of selective media for Beauveria bassiana. J Anhui Agric Univ(安徽农业大学学报), 26(1) :23～28(in Chinese)
[22] Wang B(王滨),Fan M-Z(樊美珍),Li Z-Z(李增智).2002.Population dynamics of Beauveria bassiana in a Masson pine plantation ecosystem. Chin J Appl Ecol ( 应用生态学报 ), 13 ( 11 ):1368～1372(in Chinese)
[23] Wang C-S(王成树), Li Z-Z(李增智), Fan M-Z(樊美珍). 1998.Near relation characterization of Beauveria bassiana strains isolated from different hosts and geographic origins. Chin J Appl Ecol (应用生态学报), 9(2): 189～194(in Chinese)
[24] Wang C-S(王成树), Huang B(黄勃),Wang S-B(王四宝), et al. 2002. Diversity of culture characteristics of Beauveria bassiana and the analysis of strain association with insect host and geograghic origin. Biodiv Sci(生物多样性), 10(2): 196～201 (in Chinese)
[25] Wang C-S(王成树), Shah FA, Patel N, et al. 2003. Molecular investigation on strain genetic relatedness and population structure of Beauveria bassiana. Environ Microbiol, 5 ( 10 ): 908～925
[26] Wang S-B(王四宝), Huang Y-P(黄勇平), Fan M-Z(樊美珍), et al.2003. Species diversity of entomogenous fungal flora of mountains Dabie, Anhui. Biodiv Sci (生物多样性), 11(6):475～479(in Chinese)
[27] Wang C-S(王成树), Li Z-Z(李增智), Milton AT, et al. 2003. Nuclear large subunit rDNA group Ⅰ intron distribution in a population of Beauveria bassiana strains: Phylngenetic implications. Mycol Res, 107(10): 1189～1200.
[28] Wang C-S(王成树), Fan M-Z(樊美珍), Li Z-Z(李增智), et al.2004. Molecular monitering and evaluation of application of entomopathogenic fungus Beauveria bassiana in Southeast China. J Appl Microbiol ( 应用微生物学报 ), 96: 861～870 (in Chinese)
[29] Wang S-B(王四宝),LiuJ-N(刘竞男),Huang B(黄勃),et al.2004. Entomogenous fungus communities structure and ecological distribution of mountains Dabie, Anhui Province. Mycosystem (菌物学报), 23(2): 195～203(in Chinese)
[30] Wang S-B(王四宝), Liu J-N(刘竞男), Wang C-S(王成树), et al. 2004. Community diversity of entomogenous fungi in Dabie Mountains in Anhui. Chin J Appl Ecol (应用生态学报), 15(5):883～887 (in Chinese)