菌株DLL-1降解土壤和韭菜中甲基对硫磷的研究

摘要/Abstract

摘要： 施甲基对硫磷7.5、15和22.5kg·hm^-2(a.i.)时,韭菜中最终平均农药残留量为0.633、1.270和1.901mg·kg^-1,自然降解率分别为98.94%、96.44%和96.04%.施用高效农药残留降解菌剂能显著地降低农药残留的含量,施用75kg·hm^-2降解菌剂时,韭菜与土壤中平均农药残留量分别为0.269、0.099mg·kg^-1,与不施菌对照相比,能使农药进一步降低78.82%和98.68%.降解率随着菌剂用量增加而升高,当用量超过75kg·hm^-2时降解率不再提高.菌剂施用时间以施药后3d为最好.

关键词: 降解菌, 甲基对硫磷, 农药残留, 降解率

Abstract: Degradation of methyl parathion in soil and Chinese chive by strain DLL^-1 was studied. Usage of methyl parathion at 7.5, 15, and 22.5 kg(a.i.)穐m^-2 resulted in the average amount of residue of 0.663, 1.270, and 1.901 mg穔g^-1 in Chinese chive respectively. The natural degradation rate was 98.94%, 96 44%, and 96.04% corresponding to the 3 levels of usage. The amount of pesticide residue could be significantly decreased through the application of high effective degrading microbial agents. The amount of pesticide residue in Chinese chive and soil was 0.269 and 0.099 mg穔g^-1 with the usage of 75 kg穐m^-2 of degradation bacterium, which was decreased by 78.82% and 98.68% compared with the control. Increasing the bacterium usage led to the increase of degradation rate. Usage of degradation bacterium more than 75 kg穐m^-2 did not increase the degradation rate further. The best time of the application of the degrading microbe was 3 days after the application of the pesticide.

Key words: Degrading bacterium, Methyl parathion, Pesticide residue, Degrading rate

中图分类号:

S481.8

张瑞福, 蒋建东, 崔中利, 李顺鹏. 菌株DLL^-1降解土壤和韭菜中甲基对硫磷的研究[J]. 应用生态学报, 2004, (2): 295-298.

ZHANG Ruifu, JIANG Jiandong, CUI Zhongli, LI Shunpeng. Degradation of methyl parathion in soil and Chinese chive by strain DLL^-1[J]. Chinese Journal of Applied Ecology, 2004, (2): 295-298.

参考文献

[1] Andrew MF, Olli HT. 1991. Micobiological degradation of pesticides in yard waste composting. Microbiol Rev, 55(2):225～233
[2] Brunner W, Sutherland FH, Focht DD. 1985. Enhanced biodegradation in soil by analog enrichment and bacterial inoculation. J Environ Qual, 14:324～328
[3] Cheng G-F(程国锋),Li S-P(李顺鹏),Shen B(沈标).1998.Microbial degradation of residual pesticides in vegetable. Chin J ApplEnvironBiol(应用与环境生物学报),4(1):81～84(in Chinese)
[4] Chandhry GR, Ali AN, Wheeler WB. 1988. Isolation of a methyl paration-degrading Pseudomonas sp. that possesses DNA homologous to the opd gene from a Flavobacteium sp. Appl Environ Microbiol, 54 (2): 288 ～ 293
[5] Cao Z-F(曹志方),Wang Y-S(王银善).1996.Biodegradation of metharnidophos by microbes.Adv Environ Sci(环境科学进展),4(6):32～35(in Chinese)
[6] Cui Z-L(崔中利),Li S-P(李顺鹏),He J(何健).2001.Study on the isolation of methyl parathion degrading bacillus strain J5 and the characteristics of the degradation. Rural Eco-Environ (农村生态环境),17(3):21～25(in Chinese)
[7] Cui ZL, Li SP, Fu GP. 2001. Isolation of methyl parathion-degrading strain M6 and cloning of the methyl parathion hydrolase gene.Appl Environ Microbiol, 67 (10): 4922～ 4925
[8] Dang Z-H(党志红),Dong J-Z(董建臻),GaoZ-L(高占林).2001.Biology and injury of Bradysia odoriphaga on leek in different type of cultivation.J Hebei Agric Univ(河北农业大学学报),24(4):65～68 (in Chinese)
[9] Fang L(方玲 ). 2000. Isolation and selection of strains used to degrade organic chlorine pesticides and application effects. Chin J ApplEcol(应用生态学报),1(2):249～252(in Chinese)
[10] Funk SB, Roberts DJ Crawford DL, et al. 1993. Initial-phase optimization for bioremediation of munition compound contaminated soil. Appl Environ Microbiol, 59 (7): 2171 ～ 2177
[11] Qiu J-S(邱俊珊),Zhu K-L(朱可丽),Zhang J(张杰),et al.2002. Diversity of the microorganisms degrading aromatic hydrocarbans.Chin JAppl Ecol(应用生态学报),13(12):1713～1715(in Chinese)
[12] Ka JO, Holben WE, Tiedje JM. 1994. Genetic and phenotypic diversity of 2, 4- dichlorophenoxyacetic acid( 2, 4-D )-degrading bacteria isolated from², 4-D-treated field soils. Appl Environ Microbiol, 60(4): 1106～ 1115
[13] Liu G-Q(刘国奇),Jiang R-Z(蒋如璋).1999.Isolation and preliminary study on Pseudomonas fluorescent rhizobacterial strain from chinese chives.BullMicrobiol(微生物学通报),26(3):189～192(in Chinese)
[14] Li S-P(李顺鹏),Shen B(沈标),Wei S-L(魏社林).1996 Ecological effect of bacterium to degrade parathion methyl(PM) and its application.Acta Pedol Sin(土壤学报),33(4):380～384(in Chinese)
[15] Liu Z(刘智),Sun J-C(孙建春),Li S-P(李顺鹏)1999.Isolation, identification and characters of methyl parathion on degraingbatcerium.Chin J Appl Environ Biol(应用与环境生物学报),5(Suppl.): 147～ 150(in Chinese)
[16] Middeldorp PJM, Briglia M, Salkinoja-Salonen MS. 1990. Biodegradation of pentachloropenol in natural polluted soil by inoculated Rhodococcus chlorophenolicus. Microbiol Ecol, 20:123～ 139
[17] Mulbry WW, Karns JS. 1989. Paration hydrolase specified by the Flavobacterium opd Gene: Relationship between the gene and protein. J Bact, 171 (12): 6740～ 6746
[18] Mulbry WW, Karns JS, Kearney PC, et al. 1986. Identification of a plasmid-borne parathion hydrolase gene from Flavobacteium sp. by southern hybridization with opd from Pseudomonas diminuta. Appl Environ Microbiol, 51(5) :926～930
[19] Park HS, Lim SJ, Chang YK, et al. 1999. Degradation of chloronitrobenzenes by a coculture of Pseudomonas putida and a Rhodococcus sp. Appl Environ Microbiol, 65(3): 1083 ～ 1091
[20] Rani NL, Latithakumari D. 1994. Degradation of methyl parathion by Pseudomonas putida. Can J Microbiol, 40:1000～ 1006
[21] Struthers JK, Jayachandran K, Moorman TB. 1998. Biodegradation of atrazine by Agrobacterium radiobacter J14a and use of this strain in bioremediation of contaminated soil. Appl Environ Microbiol, 64 (9): 3368 ～ 3375
[22] Yanze-Kontchou C, Gschwind N. 1994. Mineralization of the herbicide atrazine as a carbon source by a pseudomonas strain. Appl Environ Microbiol, 60(12): 4297～ 4302
[23] Zhang Q-L(张全力),Pan W-L(潘文亮),Jia H-M.(贾海民).2000. Propoed guideline for field experiment to control Bradysia odoriphaga.Pesticide SciAdmin(农药科学与管理),21(3):37～39(in Chinese)
[24] Zhu J-S(朱九生),Qiu S(秦曙),Wang J(王静).2001 Monitoring of organophosphorus pesticide residue in vegetables from 4markets of tmyuan.Pesticide Sci Admin(农药科学与管理),22(3):21～23(in Chinese)