乙酸对土壤胶体矿物吸附酸性磷酸酶的影响

应用生态学报 ›› 2004, Vol. ›› Issue (3): 482-486.

乙酸对土壤胶体矿物吸附酸性磷酸酶的影响

赵振华¹, 黄巧云², 蒋新¹, 余贵芬¹, 王芳¹, 李学垣²

1. 中国科学院南京土壤研究所, 南京 210008;
2. 华中农业大学资源与环境学院, 武汉 430070

收稿日期:2002-11-12 修回日期:2003-03-31
通讯作者: 蒋新
基金资助:
国家重点基础研究发展规划项目(2002CB410805);国家自然科学基金项目(40271064);中国科学院王宽诚博士后工作奖励基金资助项目

Effect of acetic acid on adsorption of acid phosphatase by some soil colloids and clay minerals

ZHAO Zhenhua¹, HUANG Qiaoyun², JIANG Xin¹, YU Guifen¹, WANG Fang¹, LI Xueyuan ²

1. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;
2. Huazhong Agricultural University, Wuhan 430070, China

Received:2002-11-12 Revised:2003-03-31

摘要/Abstract

摘要： 研究了不同pH值、不同浓度乙酸对酸性磷酸酶在土壤胶体和矿物表面吸附的影响.结果表明,在pH2～8的乙酸体系中,酶在胶体矿物表面的最大吸附pH一般出现在蛋白的等电点和矿物的零电荷点(PZC)之间.各土壤胶体和粘粒矿物对酶的吸附量大小顺序为针铁矿黄棕壤>砖红壤>高岭石>二氧化锰.乙酸浓度对酶在胶体矿物表面的吸附量和吸附结合能具有较显著影响,在0～200mmol·L^-1范围内,随着乙酸浓度的增加,酶吸附量呈现先升高、后降低、再稳定的趋势,而吸附结合能的变化与此相反.并就乙酸对酶在胶体矿物表面吸附影响的可能机理进行了初步探讨.

关键词: 胶体矿物, 吸附, 乙酸, 酸性磷酸酶

Abstract: This paper studied the effect of acetic acid with different concentrations and pH values on the adsorption of acid phosphatase by some soil colloids and clay minerals (SCCM).The results showed that the pH values for the maximum adsorption of the enzyme were between the IEP of the enzyme and the PZC of SCCM.In the acetic acid systems,the amount of the enzyme adsorbed by SCCM was in the order of goethiteyellow brown soil>latosol>kaolinite>δ-MnO₂.A remarkable influence of acetic acid concentration on the adsorption amount and the binding energy of the enzyme was observed.With the increase of the concentration from 0 to 200 mmol·L^-1 in the system,acetic acid exhibited an enhanced effect,followed by an inhibition action on the adsorption of the enzyme on SCCM.The changes of the binding energy (K value) for the enzyme on SCCM were on the contrary to those of the maximum adsorption.The possible mechanisms for the influence of acetic acid on the adsorption of enzyme by SCCM were also discussed.

Key words: Colloid minerals, Adsorption, Acetic acid, Acid phosphatase

中图分类号:

S154.2

赵振华, 黄巧云, 蒋新, 余贵芬, 王芳, 李学垣. 乙酸对土壤胶体矿物吸附酸性磷酸酶的影响[J]. 应用生态学报, 2004, (3): 482-486.

ZHAO Zhenhua, HUANG Qiaoyun, JIANG Xin, YU Guifen, WANG Fang, LI Xueyuan . Effect of acetic acid on adsorption of acid phosphatase by some soil colloids and clay minerals[J]. Chinese Journal of Applied Ecology, 2004, (3): 482-486.

参考文献

[1] Atkinson RJ, Posnel AM, Quisk JP.1967.Adsorption of potentialdetermining ion at ferrio aqueous electrolyte interface.J Phys Chem, 71 (3): 551 ～ 555
[2] Floyd R, Sharp DG.1978.Viral aggregation:Effects of salt on the aggregation of poliovirus and revirus at low pH.Appl Environ Microbol, 35:1084 ～ 1094
[3] Giles CH, MacEwan TH, Nakhwa SN.1960.Studies in adsorption Part Ⅺ.A system of classification of solution adsorption isotherms and its use in diagnosis of adsorption mechanisms and in measurement of specific surface area of solids.J Chen Soc, 111:3973～3993
[4] Grierson PF.1992.Organic acids in the rhizoshere of Banksia integrifolia L.F.Plant Soil, 144:259～265
[5] Huang QY,Jiang MH, Li XY.1998 Effects of iron and aluminum oxides and kaolinite on adsorption and activity of invertase.Pedosphere, 8: 251 ～ 260
[6] Lowery OH, Rosenbrough NJ, Farr AL, et al.1951.Protein measurement with Folin phenol reagent.J Biol Chem, 193:265～278
[7] Morgan HW, Corke CT.1976.Adsorption, desorption, and activity of glucose oxidase on selected clay species.Can J Microbiol, 22: 684～ 693
[8] Naidja A, Huang PM, Bollag JM.2000.Enzyme-clay interactions and their impact on transformations of natural and anthropogenic organic compounds in soil.J Environ Qual, 29 (3): 677 ～ 691
[9] Paridu KM.1981.Studies on MnO2 I.Chemical composition microstructure and other characteristics of some synthetic MnO2 of various crystalline modifications.Electrochem Acta, 26 (3): 435 ～443
[10] Quiquampoix H, Abadie J, Baron MH, et al.1995.Mechanisms and consequences of protein adsorption on soil mineral surfaces.In:Hobert TA, Brash JL, eds.Protein at Interfaces Ⅱ.ACS symposium series.No.602.Washington, D.C.: American Chemical Society.321 ～ 333
[11] Rao MA, Gianfreda L, Palmiero F.1996.Interactions of acid phosphatase with clays, organic molecules and organo-mineral complexes.Soil Sci, 161(11) :751 ～760
[12] Rao MA, V iolante A, Gianfreda L.2000.Interaction of acid phosphatase with clays, organic molecules and organo-mineral complexes: kinetics and stability.Soil Biol Biochem, 32: 1007～1014
[13] Servagent-Noinville S, Revault M, Quiquam-poix H, et al.2000.Conformational changes of bovine serum albumin induced by adsorption on different clay surfaces: FTIR analysis.J Coll Interface Sci, 221:273～283
[14] Shen Yin, Lena Strom, Jan-AKE Jonsson, et al.1996.Low-molecular organic acids in the rhizosphere soil solution of beech forest (FAGUS SYLVATICA L.) cambisols determined by ion chromatography using supported liquid membrane enrichment technique.Soil Biol Biochen, 28(9): 1163～1169
[15] Straiter N, Klabunde T, Tucker P.1995.Crystal structure of a purple acid phosphatase containing a dinuclear Fe(Ⅲ)-Zn(Ⅱ) active site.Science, 268:1489～ 1492
[16] Taylor DH, Bosmann HB.1981.The electrokinetic properties of reovirus type 3.Electrophoretic mobility and zeta potential in dilute electrolytes.J Coll Interface Sci, 83(1): 153 ～ 162