降解性细菌对菲诱导的蛋白及酶活性应答反应

摘要/Abstract

摘要： 测定了3株以菲为唯一碳源的芽孢杆菌属(Bacillus)菌株(BA11、BA19和BA27)的降解能力、多酚氧化酶和过氧化氢酶的活性及蛋白变化.结果表明,当菲浓度在200mg·L^-1以下时,3株菌多酚氧化酶和过氧化氢酶的活性随菲浓度的提高变化不大,其中BA19和BA27菌株表现出较高的稳定性.当菲浓度为200mg·L^-1时,BA27诱导表达了一条分子量为27000道尔顿的新蛋白条带,同时有些蛋白的合成受到抑制.因此可以认为,诱导产生的新蛋白与其污染条件下的细菌降解能力及稳定性有关.

关键词: 菲, 降解细菌, 酶活性, 生物修复

Abstract: Three strains of Bacillus sp.BA11,BA19 and BA27,which can grow when phenanthrene is taken as the sole source of carbon and energy,were isolated from an oil-contaminated soil.Their crude extract was used to test the activities of polyphenol oxidase and catalase.The results demonstrated that all the three strains did not show a significant change in the activity of both enzymes when phenanthrene was lower than 200 mg稬^-1 .Under different phenanthrene concentration,the enzyme activities of strains BA19,BA27 showed a higher stabilization than BA11.Using the SDS-PAGE contrasted the difference of proteins between the strains in normal medium and the strains growing on phenanthrene,the results showed that BA27 growing on phenanthrene synthesized a new protein with 27 000 dal of molecular weight.At the same time,the synthesis of some proteins was cancelled.We conjecture that the new protein induced by phenanthrene may be related to the ability of phenanthrene degradation and the stabilization of enzyme activity by the strain.

Key words: Phenanthrene, Degradative bacterium, Enzyme activity, Bioremediation

中图分类号:

X171.5

钟鸣, 周启星, 许华夏, 吴晓丹, 李玉双. 降解性细菌对菲诱导的蛋白及酶活性应答反应[J]. 应用生态学报, 2004, (5): 871-874.

ZHONG Ming, ZHOU Qixing, XU Huaxia, WU Xiaodan, LI Yushuang . Revulsive response of protein and enzyme activity of degradative Bacilluss strains by phenanthrene[J]. Chinese Journal of Applied Ecology, 2004, (5): 871-874.

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