砷对土壤脲酶活性影响的研究

应用生态学报 ›› 2004, Vol. ›› Issue (5): 895-898.

砷对土壤脲酶活性影响的研究

和文祥^1,2, 马爱生², 武永军², 朱铭莪²

收稿日期:2003-03-11 修回日期:2003-07-15
通讯作者: 和文祥,男,1968年生,博士,副教授,主要从事土壤生物化学和生态毒理学研究,发表论文20余篇.E-mail:hewenxiang@sina.com
基金资助:
国家自然科学基金重点项目(20337010);国家自然科学基金(39370148);沈阳大学沈阳环境工程重点实验室基金资助项目

Effect of arsenic on soil urease activity

HE Wenxiang^1,2, MA Aisheng², WU Yongjun², ZHU Minge ²

1. College of Resource and Environmental, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China;
2. Key Laboratory of Shengyang Environmental Engineering, Shengyang University, Shenyang 110044, China

Received:2003-03-11 Revised:2003-07-15

摘要/Abstract

摘要： 采用模拟方法对As污染土壤脲酶特征进行了研究.结果表明,土壤中As浓度在0～200mg·kg^-1浓度范围内,反应初期脲酶活性变化无明显规律;一年后砷激活土壤脲酶活性,二者达到显著或极显著正相关.随As浓度增加,土壤脲酶K_m值基本不变或略有增加,V_max增大,从机制上揭示出As加速脲酶尿素复合物的解离.厩肥和无肥土样脲酶对As的反应类似,只是变化幅度有所差异.

关键词: 土壤脲酶, 砷, 动力学特征

Abstract: By simulation method,this paper studied the urease activity in arsenic polluted soils.The results showed that arsenic could activate soil urease activity after added for one year.With arsenic addition,soil urease activity remarkably increased,and there existed a significantly positive correlation.The V_max of soil urease increased with arsenic addition,while the K_m of urease in high and low fertility soil was unchanged and slight increased,respectively.It is proved that As could accelerate the separating velocity of enzyme-urea complexes.This paper also illustrated that soil properties were important to study the relationship between soil enzyme and pollutants.

Key words: Soil urease, Arsenic, Kinetic characteristics

中图分类号:

S154.2

和文祥, 马爱生, 武永军, 朱铭莪. 砷对土壤脲酶活性影响的研究[J]. 应用生态学报, 2004, (5): 895-898.

HE Wenxiang, MA Aisheng, WU Yongjun, ZHU Minge . Effect of arsenic on soil urease activity[J]. Chinese Journal of Applied Ecology, 2004, (5): 895-898.

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