土壤微生物多样性研究方法

摘要/Abstract

摘要： 概述了研究土壤微生物多样性的主要方法.传统上,土壤微生物群落的分析依赖于培养技术,使用各种培养基最大限度地培养各种微生物群体,但仍只能培养和分离出一小部分土壤微生物群落.使用Biolog分析、磷脂脂肪酸分析和核酸分析等方法,可研究和表征那些现在还不能够被培养的土壤微生物,从而获取关于土壤微生物群落多样性的更多和更完整的信息.

关键词: 土壤, 微生物多样性, Biolog, 磷脂脂肪酸, 核酸分析

Abstract: This paper gave a review on the main methods for studying soil microbial diversity.Traditionally,the analysis of soil microbial communities relied on culturing techniques,using a variety of culture media.However,only a small fraction of the soil microbial community has been cultured and isolated with this approach.Other methods such as Biolog GN analysis,phospholipids fatty acids analysis and nucleic acid-based analysis can be used to study and characterize soil microbes which currently cannot be cultured,and to get more and complete information about soil microbial community.

Key words: Soil, Microbial diversity, Biolog, FLFA, Nucleic acid-based analysis

中图分类号:

S154.3

钟文辉, 蔡祖聪. 土壤微生物多样性研究方法[J]. 应用生态学报, 2004, (5): 899-904.

ZHONG Wenhui, CAI Zucong . Methods for studying soil microbial diversity[J]. Chinese Journal of Applied Ecology, 2004, (5): 899-904.

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