生防细菌产生的拮抗物质及其在生物防治中的作用

摘要/Abstract

摘要： 利用生防细菌防治植物病害是生物防治的一个主要内容.生防细菌防治植物病害发生发展的一个重要机制是产生拮抗物质.生防细菌的拮抗物质种类多,作用范围广谱.同一种拮抗物质可以由多种细菌菌株产生,而同一细菌也可以产生多种不同结构的拮抗物质.运用现代分子生物学技术和先进的分析测试手段可以加快对产生拮抗物质生防细菌的研究,了解生防细菌在寄主植物根围和叶围的定植效果,明确拮抗物质在生物防治中的作用.拮抗物质的产生除与细菌基因型有关外,一些外在的生物和非生物因素如病原菌存在与否、温度、pH和C、N营养等也影响拮抗物质产生.文中论述了生防细菌应用中存在的问题,指出混合菌剂的研制对防止病原菌抗性产生具有重要作用,应是今后生防菌剂研制中的重点.

关键词: 细菌, 拮抗物质, 生物防治

Abstract: Using bacteria to control plant diseases is one of the main strategies in plant protection,and its mechanism is commonly thought to be the production of antibiotics by bacteria.The produced antibiotics not only have structural diversity,but also have broad-spectrum activity against many pathogens.Experimental results showed that one kind of antibiotics could be produced by several bacterial strains,and one bacterial strain could also produced more than one kind of antibiotics.Recent development in molecular and bio-analytical techniques greatly promoted the research of bacterial bio-control,and the colonization survey of introducing bacterial strain to the rhizosphere and spermosphere of host plants.Besides bacterial genotypes,several biotic and abiotic factors including whether the pathogens existing or not,temperature,pH,carbon and nitrogen sources are also identified to affect the antibiotics production by bacteria.The authors illustrated some of the impeding problems in bacterial bio-control agents' application,and suggested that in the future research,more attention should be paid on developing mixed bio-control agent to avoid the anti-antibiotic activity of pathogens.

Key words: Bacteria, Antibiotics, Biological control

中图分类号:

S336

王光华, Jos M. Raaijmakers. 生防细菌产生的拮抗物质及其在生物防治中的作用[J]. 应用生态学报, 2004, (6): 1100-1104.

WANG Guanghua, Jos M. Raaijmakers . Antibiotics production by bacterial agents and its role in biological control[J]. Chinese Journal of Applied Ecology, 2004, (6): 1100-1104.

参考文献

[1] Anjaiah V,Koedam N,Nowak-Thompson B,et al.1998.Involvement of phenazines and anthranilate in the antagonism of Pseudomonas aeruginosa PNA1 and Tn5 derivatives toward Fusarium spp.and Pythium spp.Mol Plant-Microbe Interact,11:847～854
[2] Bender CL,Rangaswamy V,Loper JE.1999.Polyketide production by plant-associated pseudomonads.Annu Rev Phytopathol,37:175～196
[3] Bonsall RF,Weller DM,Thomashow LS.1997.Quantification of 2,4-diacetylphloroglucinol produced by fluorescent Pseudomonas spp.in vitro and in the rhizosphere of wheat.Appl Environ Microbiol,63:951～955
[4] Broderick NA,Goodman RM,Raffa KF,et al.2000.Synergy between zwittermicin A and Bacillus thuringiensis subsp kurstaki against gypsy moth.Environ Ent,29:101～107
[5] Burkhead KD,Schisler DA,Slininger PJ.1994.Pyrrolnitrin production by biological-control agent Pseudomonas cepacia B37w inculture and in colonized wounds of potatoes.Appl Environ Microbiol,60:2031～2039
[6] Chen Z-Y(陈中义),Zhang J(张杰),Huang D-F(黄大日方).2003.Research progress on antimicrobial mechanism and genetic engineering of Bacillus for plant diseases biocontrol.Acta Phytopathol Sin(植物病理学报),33(2):97～103(in Chinese)
[7] Chernin L,Brandis A,Ismailov Z,et al.1996.Pyrrolnitrin production by an Enterobacter agglomerans strains with broad spectrum activity towards fungal and bacterial phytopathogens.Curr Microbiol,33:208～2122
[8] Chin-A-Woeng TFC,Bloemberg GV,Van der Bij AJ,et al.1998.Biocontrol by phenazine-1-carboxamide producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporum f.sp.radicis-lycopersici.Mol Plant-Microbe Interact,10:79～86
[9] Cronin D,MoenneLoccoz Y,Fenton A,et al.1997.Role of 2,4-diacetylphloroglucinol in the interactions of the biocontrol pseudomonad strain F113 with the potato cyst nematode Globodera rostochiensis.Appl Environ Microbiol,63:1357～1361
[10] Duffy BK,Defago G.1999.Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains.Appl Environ Microbiol,58:2429～2438
[11] Fenton AM,Stephens PM,Crowley J,et al.1992.Exploitation of genes involved in 2,4-diacetylphloroglucinol biosynthesis to confer a new biocontrol capability to a Pseudomonas strain.Appl Environ Microbiol,58:3873～3878
[12] Fravel DR.1988.Role of antibiosis in the biocontrol of plant disease.Annu Rev Phytopathol,26:75～91
[13] Gaffney TD,Lam ST,Ligon JM,et al.1994.Global regulation of expression of antifungal factors by Pseudomonas fluorescens biological control strain.Mol Plant-Microbe Interact,7:455～463
[14] Gamard P,Sauriol F,Benhamou N,et al.1997.Novel butyrolactones with antifungal activity produced by Pseudomonas aureofaciens strain 63-28.J Antibiot,50:742～749
[15] Gardener BBM,Schroeder KL,Kalloger,et al.2002.Genotypic and phenotypic diversity of phlD-containing Pseudomonas strains isolated from the rhizosphere of wheat.Appl Environ Microbiol,66:1939～1946
[16] Georgakopoulos D,Hendson M,Panopoulos NJ,et al.1994.Cloning of a phenazine biosynthetic locus of Pseudomonas aureofaciens PGW12 and analysis of its expression in vitro with the ice nucleation reporter gene.Appl Environ Microbiol,60:2931～2938
[17] Giacomodonato MN,Pettinari MJ,Souto GI,et al.2001.A PCR-based method for the screening of bacterial strains with antifungal activity in suppressive soybean rhizosphere.World J Micr Bioech,17:51～55
[18] Gutterson NI,Layton TJ,Ziegle JS,et al.1986.Molecular cloning and genetic determinants for inhibition of fungal growth by a fluorescent pseudomonad.J Bacteriol,165:696～703
[19] Handelsman J,Stabb EV.1996.Biocontrol of soilborn plant pathogens.Plant Cell,8:1855～1869
[20] Hokeberg M,Wright SAI,Svensson M,et al.1988.Mutants of Pseudomonas chlororaphis defective in the production of an antifungal metabolite express reduced biocontrol activity.Abstract Proceedings ICPP98,Edinburgh,Scotland.
[21] Howell CR,Stipanovic RD.1979.Control of Rhizoctonia solani on cotton seedings with Pseudomonas fluorescens and with an antibiotic produced by the bacterium.Phytopathology,69:480～482
[22] Kalbe C,Marten P,Berg G.1996.Strains of the genus Serratia as beneficial rhizobacteria of oilseed rape with antifungal properties.Microbiol Res,151:433～439
[23] Kang YW,Carlson R,Tharp W,et al.1998.Characterization of genes involved in biosynthesis of a novel antibiotic from Burkholderia cepacia BC11 and their role in biological control of Rhizoctonia solani.Appl Environ Microbiol,44:3939～3947
[24] Keel C,Schnider U,Maurhofer M,et al.1992.Suppression of root diseases of by Pseudomonas fluorescens CHA0:Importance of secondary metabolite 2,4-diacetylphloroglucinol.Mol Plant-Microbe Interac,5:4～13
[25] Keel C,Weller DM,Natsch A,et al.1996.Conservation of the 2,4-diacetylphloroglucinol biosynthesis locus among fluorescent Pseudomonas strains from diverse geographic locationa.Appl Environ Microbiol,62:552～563
[26] Kim KK,Kang JG,Moon SS,et al.2000.Isolation and identification of antifungal N-butylbenzensulphonamide produced by Pseudomonas sp. AB2.J Antibiotics,53:131～136
[27] Kloepper JW,Leong J,Teintze M,et al.1980.Pseudomonas siderophores:A mechanism explaining disease suppressive soils.Curr Microbiol,4:317～320
[28] Levy E,Gough FJ,Berlin KD,et al.1992.Inhibition of Septoria tritici and other phytopathogenic fungi and bacteria by Pseudomonas fluorescens and its antibiotics.Plant Pathol,41:335～341
[29] Ligon JM,Hill DS,Hammer PE,et al.2000.Natural products with antifungal activity from Pseudomonas biocontrol bacteria.Pest Manage Sci,56:688～695
[30] Maurhofer M,Keel C,Haas D,et al.1995.Influence of plant species on disease suppression by Pseudomonas fluorescens CHA0 with enhanced antibiotic production.Plant Pathol,44:44～50
[31] Mavrodi OV,Gardener BBM,Mavrodi DV,et al.2001.Genetic diversity of phlD from²,4-diacetylphloroglucinol-producing fluorescent Pseudomonas spp.Phytopathology,91:35～43
[32] Milner JL,Silo-Suh L,Lee JC,et al.1996.Production of Kanosamine by Bacillus cereus UW85.Appl Environ Microbiol,62:3061～3065
[33] Nakayama T,Homma Y,Hashidoko Y,et al.1999.Possible role of xanthobaccins produced by Stenotrophomonas sp. strain SB-K88 in suppression of sugar beet damping-off disease.Appl Environ Microbiol,55:4334～4339
[34] Nielsen MN,Sorensen J,Fels J,et al.1998.Secondary metabolite-and endochitinase-dependent antagonism toward plant-pathogenic microfuhgi of Pseudomonas fluorescens isolates from sugar beet rhizosphere.Appl Environ Microbiol,64:3563～3569
[35] Nishida M,Matsubara T,Watanabe N.1965.Pyrrolnitrin,a new antifungal antibiotic:Microbiological and toxicological observations.J Antibiotics,18:211～219
[36] Pierson LS,Thomashow LS.1992.Cloning and heterologous expression of the phenazine biosynthetic locus from Pseudomonas aureofaciens 30-84.Plant-Microbe Interact,5:330～339
[37] Raaijmakers JM,Weller DM,Thomashow LS.1997.Frequency of antibiotic-producing Pseudomonas spp.in natural environments.Appl Environ Microbiol,63:881～887
[38] Raaijmakers JM,Bonsall RF,Weller DM.1999.Effect of population density of Pseudomonas fluorescens on production of 2,4-diacetylphloroglucinol in the rhizosphere of wheat.Phytopathology,89:470～475
[39] Raaijmakers JM,Weller DM.2001.Exploiting genotypic diversity of 2,4-diacetylphloroglucinol-producing Pseudomonas spp.characterization of superior root-colonizing P.fluorescens strain Q8r1-96.Appl Environ Microbiol,67:2545～2554
[40] Rosales AM,Thomashow LS,Cook RJ,et al.1995.Isolation and identification of antifungal metabolites produced by rice-associated antagonistic Pseudomonas spp.Phytopathology,85:1028～1032
[41] Sarniguet A,Kraus J,Henkels MD,et al.1995.The sigma factor sigma(s) affects antibiotic production and biological control activity of Pseudomonas fluorescens Pf-5.Proc Natl Acad Sci USA,92:12255～12259
[42] Sarniguet A,Lucas P,Lucas M,et al.1992.Relationship between take-all,soil conduciveness to the disease,populations of fluorescent pseudomonads and nitrogen fertilizer.Plant Soil,145:17～27
[43] Shanahan P,O'Sullivan DJ,Simpson P,et al.1992.Isolation of 2,4-diacetylphloroglucinol from a flurorescent pseudomonad and investigation of physiological parameters influencing its production.Appl Environ Microbiol,58:353～358
[44] Silo-Suh LA,Lethbridge BJ,Raffel SI,et al.1994.Biological-activities of 2 fungistatic antibiotics produced by Bacillus cereus UW85.Appl Environ Microbiol,60:2023～2030
[45] Souza JT.2002.Distribution,diversity,and activity of antibiotic-producing Pseudomonas spp.PhD thesis.Wageningen University,The Netherlands.
[46] Trejo-Estrada SR,Paszcaynski A,Crawford DL.1998.Antibiotics and enzymes produced by the biocontrol agent Streptomyces violaceusniger YCED-9.J Indust Microb Biotech,21:81～90
[47] Vincent MN,Harrison LA,Brackin JM,et al.1991.Genetic analysis of the antifungal activity of a soilborn Pseudomonas aureofaciens strain.Appl Environ Microbiol,57:2928～2934
[48] Wang G-H(王光华),Zhou K-Q(周克琴),Zhang Q-Y(张秋英),et al.2003.Antagonism of Bacillus strain BRF-1 against plant pathogenic fungi.Chin J Biol Control(中国生物防治),19(2):73～77(in Chinese)
[49] Weller DM.1983.Colonization of wheat roots by a flurorescent pseudomonad suppressive to take-all.Phytopathology,73:1548～1553
[50] Wright SAI,Zumoff CH,Schneider L,et al.2001.Pantoea agglomerans strain EH318 produces two antibiotics that inhibit Erwinia amylovora in vitro.Appl Environ Microbiol,67:284～292
[51] Yoshida S,Hiradate S,Tsukamoto T,et al.2001.Antimicrobial activity of culture filitrate of Bacillus amyloliquefaciens RC-2 isolated from mulberry leaves.Phytopathology,91:181～187