蚯蚓粪对黄瓜炭疽病的系统诱导抗性作用

摘要/Abstract

摘要： 通过试验,研究了蚯蚓粪是否诱导黄瓜产生炭疽病系统抗性.结果表明,盆栽基质中加入蚯蚓粪后,黄瓜炭疽病的发病率比种植在加有草炭基质中的发病率低得多,差异显著(P<0.05).与经典的系统获得性抗性SAR试验相比没有明显差异(P>0.05),说明盆栽基质中蚯蚓粪的加入,产生了同病原菌诱导植物产生获得性系统抗性同样的作用,控制了病害的发生.进一步试验表明,黄瓜病原菌处理叶与非处理叶中多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)的活性与对照比较均有不同程度的提高,加入蚯蚓粪的处理与对照比较,黄瓜病原菌处理叶多酚氧化酶(PPO)活性和苯丙氨酸解氨酶(PAL)活性差异显著(P<0.05).第3叶片中加入蚯蚓粪的处理与对照比较,多酚氧化酶(PPO)活性差异显著(P<0.05),表明盆栽基质中加入蚯蚓粪后,通过启动植物体内的防御酶系,从而诱导了植物产生系统抗性,控制病害的发生.

关键词: 蚯蚓粪, 系统诱导抗性, 多酚氧化酶, 过氧化物酶, 苯丙氨酸解氨酶, 黄瓜炭疽病

Abstract: Pot experiment showed that mixing earthworm feces into soil induced a significantly lower(P<0.05)cucumber anthracnose than mixing peat,but had no significant difference(P>0.05)with the treatment of system acquired resistance.The activities of polyphenol oxidase,peroxidase and phenylalanine ammonia-lyase in cucumber leaves treated with and without pathogen were increased in different degrees by mixing earthworm feces,compared with by mixing peat.All these suggested that mixing with earthworm feces could induce the system resistance of cucumber and suppress the plant disease via starting up the recovery enzyme system in plant.

Key words: Earthworm feces, Systemic induction resistance, Polyphenol oxidase, Peroxidase, Phenylalanine ammonia-lyase, Anthracnose

中图分类号:

胡艳霞, 孙振钧, 孙永明, 王车辉. 蚯蚓粪对黄瓜炭疽病的系统诱导抗性作用[J]. 应用生态学报, 2004, (8): 1358-1362.

HU Yanxia, SUN Zhenjun, SUN Yongming, WANG Chehui . Earthworm feces-induced systemic resistance of cucumber against anthracnose[J]. Chinese Journal of Applied Ecology, 2004, (8): 1358-1362.

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