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应用生态学报 ›› 2004, Vol. ›› Issue (8): 1481-1486.

• 研究论文 • 上一篇    下一篇

RAPD技术在昆虫学研究中的进展

胡艳红, 迟德富   

  1. 东北林业大学, 哈尔滨 150040
  • 收稿日期:2002-10-12 修回日期:2003-02-28
  • 通讯作者: 迟德富
  • 基金资助:
    国家自然科学基金项目(39970620);教育部高校青年教师奖(2000年度)资助项目

Research advance in application of RAPD techniques in entomology

HU Yanhong, CHI Defu   

  1. Northeast Forestry University, Harbin 150040, China
  • Received:2002-10-12 Revised:2003-02-28

摘要: 综合论述了RAPD技术在昆虫分类学、物种的亲缘关系、系统发育、分子连锁图谱的构建、有害生物鉴定、害虫抗药性诊断、分子标记辅助育种和生态学这几方面研究中的应用,指出RAPD技术存在的问题,并提出一定的相关对策,阐明随着理论的发展和试验技术的进步,分子标记技术将使昆虫分子生物学研究迈上一个新台阶.

关键词: 昆虫学, RAPD技术, 分子遗传标记

Abstract: This paper summarized the application of RAPD techniques in insect taxology and ecology,relative of species,systemic development,identification of pest,diagnosing for pest resistance,construction of molecular linkage map,and molecular assistant breeding.The problems existing in the applications of RAPD in entomology were indicated,and their countermeasures were given.It was mentioned that with the development of theories and experimental techniques,insect molecular biology would make new progress and conquer all those problems.

Key words: Entomology, RAPD technique, Molecular genetic markers

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