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不同营养状态下金鱼藻的生理响应

王俊;顾宇飞;朱增银;武斌;尹大强   

  1. 南京大学环境学院 污染控制与资源化研究国家重点实验室,南京 210093
  • 收稿日期:2004-01-13 修回日期:2004-04-13 出版日期:2005-02-18

Physiological responses of Ceratophyllum demersum under different nutritional conditions

WANG Jun,GU Yufei,Zhu Zengyin,WU Bin,YIN Daqiang   

  1. State Key Laboratory of Pollution Control and Resource Reuse,School of Environment,Nanjing University,Nanjing 210093,China

  • Received:2004-01-13 Revised:2004-04-13 Online:2005-02-18

摘要: 通过静态模拟实验,比较研究了不同营养水平(中营养、富营养、重富营养和Hoagland植物培养液)下培养的金鱼藻(Ceratophyllum demersum)的蛋白质、叶绿素含量,过氧化物酶(POD)及超氧化物酶(SOD)活性变化.研究发现,总N、总P 的变化会影响金鱼藻的生物合成,当水环境总氮浓度低于1 mg·L-1,总磷浓度低于0.1 mg·L-1时,金鱼藻茎叶的叶绿素合成较低,其茎蛋白含量迅速下降.金鱼藻在富营养水平(1 mg TN·L-1, 0.1 mg TP·L-1)下抗氧化防御酶活跃,POD、SOD活性增高.研究表明,金鱼藻较适应于富营养水环境,水体营养盐继续增加对金鱼藻有胁迫作用,过高营养盐浓度(重富营养和Hoagland培养液)影响金鱼藻的抗逆能力.

关键词: 小麦生育期, 生理发育时间, 机理模型

Abstract: With static experiments,this paper investigated the protein and chlorophyll contents,and the peroxidase (POD) and superoxide dismutase (SOD ) activities of Ceratophyllum demersum cultured in meso-,eutro- and hypertrophic and Hoagland's solutions.The results showed that the changes of total N (TN) and total P (TP) concentrations in nutrient solutions had an obvious effect on the biosynthesis of C.demersum.When the concentrations of TN and TP were less than 1 mg·L-1 and 0.1 mg·L-1,respectively,the protein and chlorophyll contents of plant declined.The plant from eutrophic solution had higher SOD and POD activities.It was indicated that C.demersum was more preferable to eutropic condition (1 mg TN·L-1 and 0.1 mg TP·L-1),and the raised concentration of nutrients (e.g.,hypertrophic and Hoagland's solutions) was a stress to C.demersum and could affect its hardiness physiology.

Key words: Wheat, Development stage, Physiological development time, Mechanistic model