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硒对水稻的生态毒理效应及临界指标研究

林匡飞1;徐小清2;金霞3;邵志慧4;项雅玲4   

  1. 1华东理工大学化学物质风险评价与控制研究中心,上海 200237;2中国科学院水生生物研究所,武汉 430072;3中国科学院武汉植物研究所,武汉 430072;4农业部环境质检中心,武汉 430070

  • 收稿日期:2004-04-10 修回日期:2004-09-13 出版日期:2005-04-18

Eco-toxicological effects of selenium and its critical value on Oryza sativa

LIN Kuangfei1,XU Xiaoqing2,JIN Xia3,SHAO Zhihui4,XIANG Yaling4   

  1. 1Research Center of Risk Assessment and Management on Hazardous Chemicals,East China University of Science and Technology,Shanghai 200237,China;2Institute of Hydrobiology,Chinese Academy of Sciences, Wuhan 430072,China;3Institute of Botany,Chinese Academy of Sciences, Wuhan 430072,China;4Agro-Environmental Monitoring Center of Wuhan, Ministry of Agriculture,Wuhan 430070,China

  • Received:2004-04-10 Revised:2004-09-13 Online:2005-04-18

摘要: 通过土壤添加硒盆栽试验,研究了黄棕壤不同浓度Se对水稻生物量、叶绿素含量、抗氧化酶系统的影响.结果表明,低浓度Se(<8 mg·kg-1)对水稻根和地上生物量、叶绿素a含量、叶绿素a/b有促进作用,并整体提高了作物抗氧化酶系统,使MDA下降,SOD、CAT、POD、GSHPx活性相应提高.而高浓度Se(>16 mg·kg-1)对水稻根和地上生物量、叶绿素a含量、叶绿素a/b有明显的抑制作用,对抗氧化酶系统产生胁迫效应,使GSH-Px明显上升,MDA上升,SOD、CAT、POD酶活性明显下降.各项生理指标与土壤Se处理浓度之间相关分析表明,土壤Se浓度与地上部干重相对百分率、根干重百分率、叶绿素a/b、MDA、SOD、CAT、POD之间有显著相关性.比较胁迫效应10%值的临界指标EC10值表明,作物地上部干重抑制率作为土壤临界值制定的依据更为可靠,EC10为21 mg·kg-1.

关键词: 文昌鸡, 种质特性, RAPD, 遗传多样性

Abstract: In a pot experiment with brown-yellow soil,this paper studied the effects of different concentration selenium on the growth and activated oxygen scavenging system of Oryza sativa.The results showed that low concentration Se (<8 mg·kg-1) had a promoting effect on the wheat biomass,chlorophylla, chlorophyll a/b,and activated oxygen-scavenging system,while high concentration Se (>16 mg·kg-1) was in adverse.Soil Se concentration had a significant correlation with root biomass,shoot biomass,chlorophyll a/b, MDA, SOD,CAT,POD.It was defined by the pot experiment that 10% inhibition rate of root or shoot biomass could be regarded as critical value,and the critical content of soil Se was estimated to be 21 mg·kg-1.

Key words: Wenchang fowl, Germplasm characteristics, RAPD, Genetic diversity