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培养条件对海洋假单胞菌脂肪酸的影响

叶芳挺1;严小军1;郑立2,3;徐继林1;陈海敏1   

  1. 1宁波大学生命科学与生物工程学院,宁波 315211;2中国科学院海洋研究所,青岛 266071;3国家海洋局第一海洋研究所,青岛 266061

  • 收稿日期:2004-04-14 修回日期:2004-08-02 出版日期:2005-05-18

Effects of culture condition on fatty acids in Pseudomonas sp

YE Fangting1,YAN Xiaojun1,ZHENG Li2,3,XU Jilin1,CHEN Haimin1    

  1. 1Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211, China;2Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;3First Institute Oceanography of SOA,Qingdao 266061,China

  • Received:2004-04-14 Revised:2004-08-02 Online:2005-05-18

摘要: 研究了不同温度条件下一株海绵附生假单胞菌(Pseudomonas sp.)在不同碳源培养基中的生长情况及脂肪酸变化.结果表明,该海洋菌生长最适温度为30 ℃,在以淀粉作为外加碳源的培养基中生长最好;实验菌含13种脂肪酸,主要是c16:1(n7)、c15:0、c16:0、c17:0、c18:1(n6)、c18:1(n9)、9,10cpc17:0和其同分异构体.在30 ℃温度条件下,不饱和脂肪酸的相对含量急剧减少.在有外加碳源(葡萄糖和淀粉)的培养基中生长的细菌,奇数脂肪酸和环丙基脂肪酸含量远比未外加碳源的低.聚类分析结果表明,两种环境因子中,温度比碳源的影响更明显.

关键词: 球孢白僵菌, 孢子乳悬剂, 桃蚜, 田间试验, 防治效果

Abstract: The investigation on the growth of Pseudomonas sp.and the dynamics of its fatty acids under various culture conditions indicated that the optimum temperature for its growth was 30 ℃,and the best carbon source was starch.13 kinds of fatty acids were detected,among which,c16:1(n7),c15:0, c16:0,c17:0,c18:1(n6),c18:1(n9),9,10cpc17:0 and iso9,10cpc17:0 were the major ones.When cultured at 30 ℃,the relative content of unsaturated fatty acids decreased significantly.Carbon source in the media was the crucial factor affecting the contents of odd- and cyclopropane fatty acids.Cluster analysis revealed that temperature had a more obvious effect than carbon source.

Key words: Beauveria bassiana, Emulsifiable conidia suspensions, Myzus persicae, Field trial, Aphid control