生防菌ZJY-1及ZJY-116的GFP标记及其在黄瓜根围的生态适应性

应用生态学报 ›› 2005, Vol. 16 ›› Issue (11): 2144-2148.

生防菌ZJY-1及ZJY-116的GFP标记及其在黄瓜根围的生态适应性

张昕¹, 张炳欣¹, 喻景权¹, 张震², 沈卫峰², 陈振宇³, 石江⁴

1. 浙江大学农业与生物技术学院, 杭州 310029;
2. 浙江农业科学院, 杭州 310021;
3. 浙江大学图书馆, 杭州 310029;
4. 杭州市蔬菜研究所, 杭州 316004

收稿日期:2005-03-02 修回日期:2005-06-13 出版日期:2005-11-15 发布日期:2005-11-15
通讯作者: 张炳欣
基金资助:
国家自然科学基金重点资助项目(30230250).

Labeling of biocontrol agents ZJY-1 and ZJY-116 gfp gene and its ecological adaptability in cucumber rhizosphere

ZHANG Xin¹, ZHANG Bingxin¹, YU Jingquan¹, ZHANG Zhen², SHEN Weifeng², CHEN Zhenyu³, SHI Jiang ⁴

1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China;
2. Zhejiang Academy of Agricultural Science, Hangzhou 310021, China;
3. Library of Zhejiang University, Hangzhou 316004, China;
4. Vegetable Institute of Hangzhou, Hangzhou 316004, China

Received:2005-03-02 Revised:2005-06-13 Online:2005-11-15 Published:2005-11-15

摘要/Abstract

摘要： 将具有绿色荧光蛋白(GFP)标记和氯霉素抗性的重组质粒pRP22-GFP导入生防菌Brevibacillus brevis ZJY-1和Bacillus subtilis ZJY-116中,用这些标记菌株处理黄瓜种子,出苗后通过定期分离计数具有上述表型的转化子,研究了2株生防菌在黄瓜根围的定殖规律.结果表明,在整个生育期2株生防菌株均能在黄瓜根围有效定殖,并在黄瓜盛花期和盛果期出现数量高峰.盆栽试验发现,引入黄瓜根围的2株生防菌有向周边杂草迁移的特性,且在前一季寄主植物死亡后,菌株可在下一季植株根围增殖.

关键词: 质粒pRP22-GFP, GFP基因, 短短芽孢杆菌, 枯草芽孢杆菌, 黄瓜根围, 定殖

Abstract: The recombined plasmid pRP22-GFP contained with gfp gene and chloramphenicol resistant was successfully introduced into two biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116.After seed inoculation,the survival and colonization of the two strains were studied by periodically retrieving the GFP-tagged strains in the cucumber rhizosphere based on the selective markers.The results showed that both the strains could successfully colonize in the rhizosphere during the whole life of cucumber,and a higher colonization level was observed during anthesis and fruition stages.In pot trials,they could migrate to the nearby non-inoculated spontaneous weed plants,and reestablish in the rhizosphere of plants subsequently grown in the same pot.

Key words: Plasmid pRP22-GFP, Gfp gene, Brevibacillus brevis, Bacillus subtili, Cucumber rhizosphere, Colonization

中图分类号:

S336

张昕, 张炳欣, 喻景权, 张震, 沈卫峰, 陈振宇, 石江. 生防菌ZJY-1及ZJY-116的GFP标记及其在黄瓜根围的生态适应性[J]. 应用生态学报, 2005, 16(11): 2144-2148.

ZHANG Xin, ZHANG Bingxin, YU Jingquan, ZHANG Zhen, SHEN Weifeng, CHEN Zhenyu, SHI Jiang . Labeling of biocontrol agents ZJY-1 and ZJY-116 gfp gene and its ecological adaptability in cucumber rhizosphere[J]. Chinese Journal of Applied Ecology, 2005, 16(11): 2144-2148.

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