一株高效菲降解菌的筛选及降解条件研究

应用生态学报 ›› 2005, Vol. 16 ›› Issue (12): 2399-2402.

一株高效菲降解菌的筛选及降解条件研究

周乐, 盛下放, 张士晋, 刘静

南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室, 南京 210095

收稿日期:2005-01-07 修回日期:2005-04-12 出版日期:2005-12-15 发布日期:2005-12-15
通讯作者: 盛下放
基金资助:
国家自然科学基金资助项目(40371070).

Screening of a phenanthrene-degrading bacterium and its degradation conditions

ZHOU Le, SHENG Xiafang, ZHANG Shijin, LIU Jing

Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China

Received:2005-01-07 Revised:2005-04-12 Online:2005-12-15 Published:2005-12-15

摘要/Abstract

摘要： 从南京某石化厂排污口附近采集土样,以菲为碳源的选择性培养基分离筛选到一株菲高效降解菌F10a,根据形态和生理生化特性初步鉴定为芽孢杆菌属,并对其降解菲的特性及各种影响因素进行了研究.结果表明,F10a在50 mg·L^-1的条件下,28 ℃振荡培养27 h,菲的降解率达到98.12%;静置培养8 h,菲的降解率达到98.7%.pH值分别为、6、8时,F10a对菲具有良好的降解效能;pH值为10时F10a不生长.Zn²⁺与Pb²⁺的存在不影响F10a的降解效能,Cu²⁺可以延缓菲的降解,Cr²⁺对F10a有毒性.F10a在菲浓度为200 mg·L^-1时,28 ℃振荡培养8 h,降解率为99.6%.菲的降解程度与细菌数量的增长呈正相关关系.

关键词: 降解, 菲, 多环芳烃, 芽孢杆菌

Abstract: Several PAHs-degrading bacteria were isolated from the soil near a petrochemicals factory,and one strain F10a identified as B.sphaericus was chosen for use.The study on the phenanthrene-degradation potential of the strain and its affecting factors showed that at 28 ℃,the degradation rate of phenanthrene (50 mg·L^-1) was 98.12% after 27 hours rotary culture,and 98.47% after 84 hours static culture.F10a had a good phenanthrene-degradation capability when the pH was 4,6 and 8,but its growth was inhibited when pH was 10.Cr²⁺ was toxic to the strain,Cu²⁺ could delay the degradation of phenanthrene,while Zn²⁺ and Pb²⁺ had no significant effects.The degradation rate of phenanthrene (200 mg·L^-1) was 99.6% after 84 hours rotary culture.A significant positive relationship was found between bacterial growth and phenanthrene degradation.

Key words: Degradation, Phenanthrene, Polycyclic aromatic hydrocarbons, Bsphaericus

中图分类号:

Q93
X172

周乐, 盛下放, 张士晋, 刘静. 一株高效菲降解菌的筛选及降解条件研究[J]. 应用生态学报, 2005, 16(12): 2399-2402.

ZHOU Le, SHENG Xiafang, ZHANG Shijin, LIU Jing . Screening of a phenanthrene-degrading bacterium and its degradation conditions[J]. Chinese Journal of Applied Ecology, 2005, 16(12): 2399-2402.

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