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应用生态学报 ›› 2005, Vol. 16 ›› Issue (4): 589-594.

• 研究论文 •    下一篇

落叶松幼苗光合特性对氮和磷缺乏的响应

郭盛磊, 阎秀峰, 白冰, 于爽   

  1. 东北林业大学生命科学学院, 哈尔滨 150040
  • 收稿日期:2004-08-08 修回日期:2004-12-08 出版日期:2005-04-15 发布日期:2005-04-15
  • 通讯作者: 阎秀峰
  • 基金资助:
    国家自然科学基金重点资助项目(30130160).

Responses of larch seedling's photosynthetic characteristics to nitrogen and phosphorus deficiency

GUO Shenglei, YAN Xiufeng, BAI Bing, YU Shuang   

  1. College of Life Sciences, Northeast Forestry University, Harbin 150040, China
  • Received:2004-08-08 Revised:2004-12-08 Online:2005-04-15 Published:2005-04-15

摘要: 为探讨落叶松幼苗光合特性对氮、磷营养缺乏的响应规律,在温室内用砂培方法培养和处理落叶树幼苗,测定了净光合速率和叶绿素荧光等反映其光合能力的参数,分析了落叶松幼苗的光合特性对氮、磷营养缺乏的响应规律.结果表明,氮素供应不足(1mmol·L-1)时,落叶松幼苗针叶的全氮含量、叶绿素含量和净光合速率与对照相比都显著降低,分别下降了37%、31%和59%,同时,Fv/Fm(反映光系统II最大光能转换效率)和Fv/Fo(反映光系统II潜在活性)也分别下降了22%和57%,而叶片可溶性蛋白含量只有微弱下降.磷素供应不足(1/8mmol·L-1)时,叶片全氮和全磷含量、叶绿素含量、叶片可溶性蛋白含量和Fv/Fm与对照相比差异均不显著,净光合速率和Fv/Fo下降13%.氮、磷同时缺乏时,上述各项指标的变化与单独缺氮处理的相似,这意味着本实验中缺磷处理(1/8mmol·L-1)对落叶松幼苗光合特性影响相对较小.

关键词: 落叶松, 净光合速率, 叶绿素荧光参数, 氮,

Abstract: In this paper,the responses of larch seedling's photosynthetic characteristics to N and P deficiency were studied in greenhouse from April to September,2002.On April 10,one-year-old larch seedlings,after their roots washed and sterilized,were transplanted into a plastic pot (diameter 30 cm,height 27 cm) containing sterilized and washed quartz sand,and supplied with complete nutrient solution (4 mmol NH4NO3·L-1,1 mmol KH2PO4·L-1,1 mmol KCl·L-1,1 mmol CaCl2·L-1,0.6 mmol MgSO4·L-1,0.02 mmol FeCl3·L-1,6 μmol MnCl2·L-1,0.016 mmol H3BO3·L-1,0.3 μmol ZnCl2·L-1,0.3 μmol CuCl2·L-1,and 0.3 μmol NaMoO4·L-1).On May 20,the seedlings were treated with four different combinations of N and P supply,i.e.,control (CK,8 mmol N·L-1 and 1 mmol P·L-1),low nitrogen supply (LN,1 mmol N·L-1 and 1 mmol P·L-1),low phosphorus supply (LP,8 mmol N·L-1 and 1/8 mmol P·L-1),and low nitrogen with low phosphorus supply (LN-LP,1 mmol N·L-1 and 1/8 mmol P·L-1).In treatment LN,the other nutrients were given as CK,while in treatment LP,the K level was adjusted by KCl,with the other nutrients kept as CK.On September 4,the net photosynthetic rate,chlorophyll fluorescence parameters,chlorophyll content,total soluble protein content,and N and P contents of the needle were determined.The results showed that in treatment LN,the N content,chlorophyll content,net photosynthetic rate (Pn),maximal PSII efficiency (Fv/Fm) and potential PSII activity (Fv/Fo) of the needle declined significantly by 37%,31%,58%,22% and 57%,respectively,while in treatment LP,the N and P contents,chlorophyll content,total soluble protein content and Fv/Fm had no significant differences with CK,but the Pn and Fv/Fo was 13% lower than CK.The variation tendency and extent of Pn,chlorophyll fluorescence parameters,and other physiological indices in treatment LN-LP were similar to those in treatment LN.

Key words: Larix gmelinii, Net photosynthetic rate, Chlorophyll fluorescence parameter, N,P

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