硒对水稻的生态毒理效应及临界指标研究

应用生态学报 ›› 2005, Vol. 16 ›› Issue (4): 678-682.

硒对水稻的生态毒理效应及临界指标研究

林匡飞¹, 徐小清², 金霞³, 邵志慧¹, 项雅玲⁴

1. 华东理工大学化学物质风险评价与控制研究中心, 上海 200237;
2. 中国科学院水生生物研究所, 武汉 430072;
3. 中国科学院武汉植物研究所, 武汉 430072;
4. 农业部环境质检中心, 武汉 430070

收稿日期:2004-05-02 修回日期:2005-01-17 出版日期:2005-04-15 发布日期:2005-04-15
通讯作者: 林匡飞,男,1963年生,博士,副教授.主要从事污染生态学和生态风险研究,发表论文22余篇.E-mail:kflin@ecust.edu.cn;Tel:021-64252978
基金资助:
国家自然科学基金资助项目(30070026).

Eco-toxicological effects of selenium and its critical value on Oryza sativa

LIN Kuangfei¹, XU Xiaoqing², JIN Xia³, SHAO Zhihui¹, XIANG Yaling ⁴

1. Research Center of Risk Assessment and Management on Hazardous Chemicals, East China University of Science and Technology, Shanghai 200237, China;
2. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
3. Institute of Botany, Chinese Academy of Sciences, Wuhan 430072, China;
4. Agro-Environmental Monitoring Center of Wuhan, Ministry of Agriculture, Wuhan 430070, China

Received:2004-05-02 Revised:2005-01-17 Online:2005-04-15 Published:2005-04-15

摘要/Abstract

摘要： 通过土壤添加硒盆栽试验,研究了黄棕壤不同浓度Se对水稻生物量、叶绿素含量、抗氧化酶系统的影响.结果表明,低浓度Se(<8mg·kg^-1)对水稻根和地上生物量、叶绿素a含量、叶绿素a/b有促进作用,并整体提高了作物抗氧化酶系统,使MDA下降,SOD、CAT、POD、GSHPx活性相应提高.而高浓度Se(>16mg·kg^-1)对水稻根和地上生物量、叶绿素a含量、叶绿素a/b有明显的抑制作用,对抗氧化酶系统产生胁迫效应,使GSHPx明显上升,MDA上升,SOD、CAT、POD酶活性明显下降.各项生理指标与土壤Se处理浓度之间相关分析表明,土壤Se浓度与地上部干重相对百分率、根干重百分率、叶绿素a/b、MDA、SOD、CAT、POD之间有显著相关性.比较胁迫效应10%值的临界指标EC10值表明,作物地上部干重抑制率作为土壤临界值制定的依据更为可靠,EC10为21mg·kg^-1.

关键词: 水稻, 硒污染, 抗氧化酶系统, 生态毒理效应, 临界指标

Abstract: In a pot experiment with brown-yellow soil,this paper studied the effects of different concentration selenium on the growth and activated oxygen- scavenging system of Oryza sativa.The results showed that low concentration Se (<8 mg穔g^-1) had a promoting effect on the wheat biomass,chlorophyll a,chlorophyll a/b,and activated oxygen- scavenging system,while high concentration Se (>16 mg穔g^-1) was in adverse.Soil Se concentration had a significant correlation with root biomass,shoot biomass,chlorophyll a/b,MDA,SOD,CAT,POD.It was defined by the pot experiment that 10% inhibition rate of root or shoot biomass could be regarded as critical value,and the critical content of soil Se was estimated to be 21 mg穔g^-1.

Key words: Oryza sativa, Selenium pollution, Anti-oxidative enzymes, Ecological effect, Critical value

中图分类号:

X171.5

林匡飞, 徐小清, 金霞, 邵志慧, 项雅玲. 硒对水稻的生态毒理效应及临界指标研究[J]. 应用生态学报, 2005, 16(4): 678-682.

LIN Kuangfei, XU Xiaoqing, JIN Xia, SHAO Zhihui, XIANG Yaling . Eco-toxicological effects of selenium and its critical value on Oryza sativa[J]. Chinese Journal of Applied Ecology, 2005, 16(4): 678-682.

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